##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940130_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 709537 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70345309687867 34.0 31.0 34.0 31.0 34.0 2 32.73139103387138 34.0 31.0 34.0 31.0 34.0 3 32.81807854981488 34.0 31.0 34.0 31.0 34.0 4 36.242714615305474 37.0 37.0 37.0 35.0 37.0 5 36.211762036370196 37.0 37.0 37.0 35.0 37.0 6 36.280871892515826 37.0 37.0 37.0 35.0 37.0 7 36.24215368613617 37.0 37.0 37.0 35.0 37.0 8 36.23075752215882 37.0 37.0 37.0 35.0 37.0 9 38.018785489692576 39.0 39.0 39.0 35.0 39.0 10-11 37.99723129308267 39.0 39.0 39.0 35.0 39.0 12-13 38.0044937755184 39.0 39.0 39.0 35.0 39.0 14-15 39.48030335274975 41.0 39.0 41.0 36.0 41.0 16-17 39.4751852264223 41.0 39.0 41.0 36.0 41.0 18-19 39.5174296759718 41.0 39.5 41.0 36.0 41.0 20-21 39.50774448689779 41.0 40.0 41.0 36.0 41.0 22-23 39.47971916897921 41.0 39.0 41.0 36.0 41.0 24-25 39.45275369712925 41.0 39.0 41.0 36.0 41.0 26-27 39.33507413989686 41.0 39.0 41.0 36.0 41.0 28-29 39.22361695020838 41.0 39.0 41.0 35.0 41.0 30-31 39.04810319969219 41.0 39.0 41.0 35.0 41.0 32-33 38.846459028916044 41.0 38.0 41.0 35.0 41.0 34-35 38.712353266989595 40.5 38.0 41.0 35.0 41.0 36-37 38.57188560991182 40.0 38.0 41.0 35.0 41.0 38-39 38.40080644138361 40.0 38.0 41.0 34.0 41.0 40-41 38.25917464487405 40.0 38.0 41.0 34.0 41.0 42-43 38.05798922395872 40.0 38.0 41.0 33.5 41.0 44-45 38.07548443562492 40.0 38.0 41.0 34.0 41.0 46-47 37.80353737719104 40.0 38.0 41.0 33.0 41.0 48-49 37.49332029196504 40.0 37.5 41.0 33.0 41.0 50-51 37.29422003362756 40.0 37.0 41.0 32.0 41.0 52-53 37.14351330515534 40.0 37.0 41.0 32.0 41.0 54-55 36.94399023588622 40.0 36.0 41.0 31.5 41.0 56-57 36.757238170807156 40.0 36.0 41.0 31.0 41.0 58-59 36.522771187408125 39.5 35.0 41.0 31.0 41.0 60-61 36.266792288492354 39.0 35.0 41.0 31.0 41.0 62-63 36.013470051597025 39.0 35.0 41.0 30.5 41.0 64-65 35.72520108183224 38.0 35.0 41.0 30.0 41.0 66-67 35.41395163324816 37.0 35.0 40.0 30.0 41.0 68-69 35.07127676217026 37.0 35.0 40.0 29.0 41.0 70-71 34.81432046531752 36.0 35.0 39.0 29.5 41.0 72-73 34.63568355138632 36.0 35.0 39.0 30.0 41.0 74-75 34.319855060412635 35.5 35.0 38.5 30.0 40.5 76-77 33.98542218376208 35.0 35.0 37.0 30.0 39.5 78-79 33.6694520511263 35.0 35.0 37.0 29.0 39.0 80-81 33.37967364633557 35.0 34.0 36.5 29.0 39.0 82-83 33.128642340004816 35.0 34.0 36.0 29.0 37.0 84-85 32.91521654261864 35.0 34.0 36.0 29.0 37.0 86-87 32.72211174329175 35.0 34.0 35.0 29.0 37.0 88-89 32.55741138235216 35.0 34.0 35.0 29.0 36.0 90-91 32.44374711960053 35.0 34.0 35.0 29.0 36.0 92-93 32.31963026593398 35.0 34.0 35.0 29.0 36.0 94-95 32.223730404475035 35.0 34.0 35.0 27.5 36.0 96-97 32.15193781296817 35.0 34.0 35.0 27.0 35.5 98-99 32.08214159374353 35.0 34.0 35.0 27.0 35.0 100-101 32.04149396014584 35.0 34.0 35.0 27.0 35.0 102-103 31.262972896409913 34.5 32.5 35.0 25.0 35.0 104-105 31.87514322720309 35.0 34.0 35.0 27.0 35.0 106-107 31.930776689587717 35.0 34.0 35.0 27.0 35.0 108-109 31.92590167954596 35.0 34.0 35.0 27.0 35.0 110-111 31.88755836552569 35.0 34.0 35.0 27.0 35.0 112-113 31.827464247812305 35.0 34.0 35.0 26.5 35.0 114-115 31.753611862383497 35.0 34.0 35.0 25.0 35.0 116-117 31.67604015012607 35.0 34.0 35.0 25.0 35.0 118-119 31.592117817675472 35.0 34.0 35.0 25.0 35.0 120-121 31.48265347684476 35.0 34.0 35.0 25.0 35.0 122-123 31.315493060967928 35.0 34.0 35.0 24.0 35.0 124-125 29.606816839713787 33.5 30.5 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19338593656178205 1101 2 0.14197025615941783 1101 3 0.11292297596296663 1101 4 -0.034522945618086 1101 5 0.09438457227049923 1101 6 0.24640560199546258 1101 7 0.21206962430617438 1101 8 0.1506204367526891 1101 9 0.2951642991481691 1101 10-11 0.24316673513569498 1101 12-13 0.19475512857290056 1101 14-15 0.26601630108788754 1101 16-17 0.28804888508772564 1101 18-19 0.2370670458093045 1101 20-21 0.32940557192875985 1101 22-23 0.28555311229014535 1101 24-25 0.31673263952507824 1101 26-27 0.21544998580472452 1101 28-29 0.2741918129485157 1101 30-31 0.30790589507866173 1101 32-33 0.2308626127747786 1101 34-35 0.12245098672324417 1101 36-37 0.010982729462618579 1101 38-39 0.051034052961441034 1101 40-41 0.16326544115095487 1101 42-43 0.016165263257164497 1101 44-45 0.0332127806509348 1101 46-47 -0.10115855643141458 1101 48-49 -0.05275404937852102 1101 50-51 -0.08875855265034005 1101 52-53 -0.07994117376992449 1101 54-55 0.007385935880698469 1101 56-57 0.04751483444017879 1101 58-59 0.21926039037195721 1101 60-61 0.0640446753842383 1101 62-63 0.11015251501707723 1101 64-65 -0.009112596403390683 1101 66-67 -0.18685595815767897 1101 68-69 -0.08397546301654302 1101 70-71 0.044726308671130255 1101 72-73 0.11870356462444676 1101 74-75 0.1581385733317191 1101 76-77 0.04538184501601705 1101 78-79 0.017222076965488498 1101 80-81 0.0505981637073063 1101 82-83 0.0432717424035971 1101 84-85 -0.027734093052707465 1101 86-87 -0.13444654204051432 1101 88-89 -0.1092237457874532 1101 90-91 -0.1595375547393587 1101 92-93 -0.10090396024989445 1101 94-95 -0.11986533489481843 1101 96-97 -0.10693938414164705 1101 98-99 -0.12963303825429406 1101 100-101 -0.23413387426990795 1101 102-103 -0.22064674122412953 1101 104-105 -0.24639843043239296 1101 106-107 -0.21566187690097394 1101 108-109 -0.18642161405495017 1101 110-111 -0.21321579838839 1101 112-113 -0.29211904923248966 1101 114-115 -0.1375440537228485 1101 116-117 -0.11498897541166286 1101 118-119 -0.13252617670424627 1101 120-121 -0.35935790621913455 1101 122-123 -0.2900202247886057 1101 124-125 -0.26113076842132443 1102 1 0.1208401820797107 1102 2 0.14809419789248324 1102 3 0.12868025932949934 1102 4 -0.02866746130533926 1102 5 -0.06400919640479685 1102 6 -0.00722440920971934 1102 7 0.11222291738386048 1102 8 -0.026713197460459526 1102 9 0.07407599611032367 1102 10-11 0.10501232154008022 1102 12-13 0.08429268148624658 1102 14-15 0.16330215766159029 1102 16-17 0.2324548549582417 1102 18-19 0.3012089158292284 1102 20-21 0.22682565721262904 1102 22-23 0.262957946079986 1102 24-25 0.2781020058099344 1102 26-27 0.20190066745313118 1102 28-29 0.12745454327719585 1102 30-31 0.16841308506869979 1102 32-33 0.19647477350684994 1102 34-35 0.18388493841647602 1102 36-37 0.19432163944767922 1102 38-39 0.21463550652219965 1102 40-41 0.2090802288750595 1102 42-43 0.18891566788863656 1102 44-45 0.3184273909596982 1102 46-47 0.2853131349281455 1102 48-49 0.36730983570613773 1102 50-51 0.21313154321619265 1102 52-53 0.2565954920667295 1102 54-55 0.39146290923726923 1102 56-57 0.3035609624282287 1102 58-59 0.060489225033308 1102 60-61 0.07532961375575553 1102 62-63 0.202700292656516 1102 64-65 0.20934171136553203 1102 66-67 0.21918589007439238 1102 68-69 0.19477087973744034 1102 70-71 0.3382786617587783 1102 72-73 0.3834200891513362 1102 74-75 0.36622225423311505 1102 76-77 0.3004805906584451 1102 78-79 0.24778225811588328 1102 80-81 0.21913689938958214 1102 82-83 0.1485089965121631 1102 84-85 0.08586035727099528 1102 86-87 0.080813511495343 1102 88-89 -0.09125849740598113 1102 90-91 0.11325798186422276 1102 92-93 -0.1372040723016852 1102 94-95 0.05381494398964293 1102 96-97 -0.06876201549125227 1102 98-99 0.13068506433534566 1102 100-101 0.14976801816037266 1102 102-103 -0.03734284742432692 1102 104-105 -0.04208288013358796 1102 106-107 -0.12765647662707025 1102 108-109 -0.07683262625614518 1102 110-111 -0.028143587233010692 1102 112-113 0.04599918097169464 1102 114-115 0.05846098860782689 1102 116-117 -0.17396984879313493 1102 118-119 0.002566266024835784 1102 120-121 -0.06946668983467319 1102 122-123 0.06058711149127305 1102 124-125 0.03123103436752217 1103 1 0.07269410073050153 1103 2 0.030317660194072005 1103 3 -0.038637449003836366 1103 4 0.03710324802005971 1103 5 0.013790282761867445 1103 6 0.075628393369648 1103 7 0.13087061083624008 1103 8 0.1630034444046231 1103 9 0.1351654058325451 1103 10-11 -0.036093209213888144 1103 12-13 0.14383974249815168 1103 14-15 0.11617914178857802 1103 16-17 0.16891628848998863 1103 18-19 0.0996030130514498 1103 20-21 0.13356393846262904 1103 22-23 0.21250996740935335 1103 24-25 0.24420133616707318 1103 26-27 0.19087171159995364 1103 28-29 0.24189836768195505 1103 30-31 0.22510021304488959 1103 32-33 0.07972905178066014 1103 34-35 0.1572542340514005 1103 36-37 0.12362936514212208 1103 38-39 0.16869831654204148 1103 40-41 0.34062939802188197 1103 42-43 0.2638629645156243 1103 44-45 0.34225241576127985 1103 46-47 0.0036523586384618056 1103 48-49 0.0620565494958214 1103 50-51 -0.20014040618856654 1103 52-53 0.02702579960641316 1103 54-55 -0.2631904885801859 1103 56-57 -0.22486785701621415 1103 58-59 -0.24593438607780627 1103 60-61 -0.19120219427995266 1103 62-63 -0.22907984127205339 1103 64-65 -0.24756553069796183 1103 66-67 -0.2065411064533862 1103 68-69 -0.11404763464748413 1103 70-71 0.0612139296159242 1103 72-73 0.02397533468704438 1103 74-75 0.13444367040374772 1103 76-77 0.06715190762272982 1103 78-79 0.10235970107223125 1103 80-81 -0.08053602967787299 1103 82-83 -0.17463243453942567 1103 84-85 -0.11655934759011899 1103 86-87 -0.24239767402053047 1103 88-89 -0.23007763183058216 1103 90-91 -0.23407274730244865 1103 92-93 -0.2903228719048627 1103 94-95 -0.17783188140717954 1103 96-97 -0.0813255695587145 1103 98-99 -0.029223169791634973 1103 100-101 -0.012186346918994673 1103 102-103 -0.004038965975915687 1103 104-105 -0.16004542973676195 1103 106-107 -0.014646493988180964 1103 108-109 -0.10006861335931916 1103 110-111 -0.021943190407611723 1103 112-113 -0.017469631032273725 1103 114-115 -0.0035739816302680083 1103 116-117 -0.05117874016218238 1103 118-119 -0.04743993437199023 1103 120-121 0.03604856314151661 1103 122-123 0.01643253559841895 1103 124-125 -0.19210725928326866 1104 1 0.049756216520947305 1104 2 -0.03061109473757284 1104 3 0.0685398149653409 1104 4 -0.04686087712904197 1104 5 -0.11036674999343177 1104 6 0.017870800929969732 1104 7 0.006180272273070386 1104 8 0.12759308565610894 1104 9 0.14596341482419461 1104 10-11 0.2191308939148442 1104 12-13 0.2708697452506996 1104 14-15 0.23248568367314704 1104 16-17 0.24688502415381208 1104 18-19 0.21486874844001846 1104 20-21 0.19937559549538264 1104 22-23 0.22526056379434323 1104 24-25 0.23489428964904846 1104 26-27 0.393353017224328 1104 28-29 0.3493938947945381 1104 30-31 0.20966014973632952 1104 32-33 0.2111975351277522 1104 34-35 0.3463856910657981 1104 36-37 0.30641276270152673 1104 38-39 0.43918184714117814 1104 40-41 0.39005193678691086 1104 42-43 0.40178559503218025 1104 44-45 0.49790603077183704 1104 46-47 0.3991014824112824 1104 48-49 0.1745554026016123 1104 50-51 0.27485443278747823 1104 52-53 0.3319018203422601 1104 54-55 0.3457300545889055 1104 56-57 0.20934055659971307 1104 58-59 0.2676333724321509 1104 60-61 0.03807629457223527 1104 62-63 0.3251883255322383 1104 64-65 0.3248809093424043 1104 66-67 0.2527756886654302 1104 68-69 0.1738199449469775 1104 70-71 0.1644430102655221 1104 72-73 0.10870214448611648 1104 74-75 0.08923883509775266 1104 76-77 0.19762259300670593 1104 78-79 0.1792732940624191 1104 80-81 0.10328817437753912 1104 82-83 0.2783363011243978 1104 84-85 0.11094145523583165 1104 86-87 0.15873717780073804 1104 88-89 0.1422443701879459 1104 90-91 0.1664571178228016 1104 92-93 -0.00354484181035275 1104 94-95 0.07844162992413573 1104 96-97 0.14735653430403062 1104 98-99 0.1775073788825523 1104 100-101 0.17544841315073967 1104 102-103 0.23951262132567308 1104 104-105 0.23445177184136767 1104 106-107 0.26126471346204383 1104 108-109 0.15834959014559402 1104 110-111 0.08646985207334623 1104 112-113 0.2971836628478961 1104 114-115 0.3442532272879859 1104 116-117 0.2337355868402966 1104 118-119 0.299332131872621 1104 120-121 0.2633228543608972 1104 122-123 0.30746267597826815 1104 124-125 0.5393445940977699 1105 1 -0.08317675134287583 1105 2 -0.08138752983390418 1105 3 -0.02601979239883434 1105 4 -0.09742906214516722 1105 5 -0.2971680100002203 1105 6 0.004609398300019052 1105 7 -0.18289579153775293 1105 8 -0.04872531558363136 1105 9 -0.04500472291353219 1105 10-11 0.10907166459022477 1105 12-13 0.12929205389983167 1105 14-15 -0.005119215269274946 1105 16-17 0.019555671961647647 1105 18-19 0.16149106346798447 1105 20-21 0.17546388977713434 1105 22-23 0.11517037837672461 1105 24-25 0.15179453410838306 1105 26-27 0.170093922230393 1105 28-29 0.20866327471585322 1105 30-31 -0.08492928804474076 1105 32-33 0.11404652755383182 1105 34-35 0.10672033581180074 1105 36-37 0.004069292654833134 1105 38-39 0.1898919611534069 1105 40-41 0.14683554244005137 1105 42-43 -0.08008023188010327 1105 44-45 -0.10827336822968903 1105 46-47 -0.2573327884226302 1105 48-49 -0.3961647299279889 1105 50-51 -0.290262177876798 1105 52-53 -0.32484810883395454 1105 54-55 -0.2477367088859026 1105 56-57 -0.20575203964090605 1105 58-59 -0.13543184361323313 1105 60-61 -0.026423539390549422 1105 62-63 -0.12944411307620385 1105 64-65 0.004569772465821131 1105 66-67 0.07765707054542048 1105 68-69 -0.14134966630077628 1105 70-71 -0.08374277507179784 1105 72-73 -0.11563900666779858 1105 74-75 -0.24384356626956105 1105 76-77 -0.19446305968843802 1105 78-79 -0.19934786527141313 1105 80-81 -0.35074437267103065 1105 82-83 -0.19842505366432306 1105 84-85 -0.2461675646192205 1105 86-87 -0.3304638360582075 1105 88-89 -0.1272073129792517 1105 90-91 -0.22575564246635338 1105 92-93 -0.1752681393659543 1105 94-95 -0.2240270715897168 1105 96-97 -0.34505265646618 1105 98-99 -0.19562564038714925 1105 100-101 -0.1497542554520912 1105 102-103 -0.20157793368893095 1105 104-105 -0.1929641636048629 1105 106-107 -0.19817389310102485 1105 108-109 -0.2682113004893516 1105 110-111 -0.2906344587033125 1105 112-113 -0.21362048264198563 1105 114-115 -0.15568861277970925 1105 116-117 -0.2016792154253153 1105 118-119 -0.2269420865798395 1105 120-121 -0.2970152612197765 1105 122-123 -0.3085692031691458 1105 124-125 -0.13716751723909937 1106 1 0.007105023569586422 1106 2 -0.08874622218948502 1106 3 -0.054990711620355626 1106 4 -0.0900741256616584 1106 5 -0.1150870878585124 1106 6 -0.052228213754077046 1106 7 0.03439267539977209 1106 8 -0.1816099474001902 1106 9 -0.06987986364136134 1106 10-11 -0.16734502688084518 1106 12-13 -0.08565697422132246 1106 14-15 -0.1067376205447772 1106 16-17 -0.09908387605670299 1106 18-19 -0.10167538081976346 1106 20-21 0.06217411204025325 1106 22-23 -0.21618439284841173 1106 24-25 -0.23164760996875344 1106 26-27 -0.0847119508267582 1106 28-29 -0.17655774427102244 1106 30-31 -0.02971421272541619 1106 32-33 -0.13059032187044295 1106 34-35 -0.17282499709461518 1106 36-37 -0.2611779047603733 1106 38-39 -0.12507875712896066 1106 40-41 -0.1840502142601892 1106 42-43 -0.08956850370007885 1106 44-45 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16-17 -0.37108623130122 2216 18-19 -0.5864806473541151 2216 20-21 -0.570625506449062 2216 22-23 -0.5286995490577411 2216 24-25 -0.4485401632922503 2216 26-27 -0.5727873089188193 2216 28-29 -0.6226924749629177 2216 30-31 -0.7727508723009393 2216 32-33 -0.8413380714521494 2216 34-35 -1.0134595343547588 2216 36-37 -0.8266235317833974 2216 38-39 -0.8864066803207322 2216 40-41 -0.9867911868366761 2216 42-43 -1.108589292950299 2216 44-45 -0.8608765474864697 2216 46-47 -0.8201157805207586 2216 48-49 -0.6489734610505877 2216 50-51 -0.7264768133334272 2216 52-53 -0.6524388198550568 2216 54-55 -0.6831295463451497 2216 56-57 -0.6619940601747984 2216 58-59 -0.7280739057488574 2216 60-61 -0.8574095413903748 2216 62-63 -0.8180539191286016 2216 64-65 -0.6683226862374951 2216 66-67 -0.640574724806811 2216 68-69 -0.6065487527005331 2216 70-71 -0.6435387136617408 2216 72-73 -0.5361367042412226 2216 74-75 -0.5652898324731481 2216 76-77 -0.5821031686718712 2216 78-79 -0.47563659767154576 2216 80-81 -0.6229519587632808 2216 82-83 -0.573164531005709 2216 84-85 -0.540798320583292 2216 86-87 -0.6084219608028079 2216 88-89 -0.5383306989671652 2216 90-91 -0.6829443480874176 2216 92-93 -0.5687001430544179 2216 94-95 -0.5331541476840336 2216 96-97 -0.677909917483472 2216 98-99 -0.6524605674981281 2216 100-101 -0.7707665697286075 2216 102-103 -0.8361812390612045 2216 104-105 -0.9125869145112162 2216 106-107 -0.7253657024733684 2216 108-109 -0.6765167423726304 2216 110-111 -0.6427751053154331 2216 112-113 -0.8334093129044611 2216 114-115 -1.018275962086033 2216 116-117 -1.1509787421646642 2216 118-119 -1.0695778019945053 2216 120-121 -0.9606198056357691 2216 122-123 -0.9892820504883346 2216 124-125 -1.4893679458679685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 21.0 5 46.0 6 107.0 7 220.0 8 457.0 9 747.0 10 1356.0 11 1888.0 12 2421.0 13 3646.0 14 6257.0 15 6523.0 16 2710.0 17 2096.0 18 2073.0 19 1884.0 20 1763.0 21 1769.0 22 1819.0 23 2118.0 24 2413.0 25 3171.0 26 3962.0 27 4887.0 28 6037.0 29 7633.0 30 9604.0 31 12187.0 32 15803.0 33 20402.0 34 29368.0 35 48786.0 36 106118.0 37 255938.0 38 142354.0 39 943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.60710273048561 18.662711933698077 13.529077578271147 22.20110775754517 2 15.954425591349658 20.531316197829238 39.79698980016095 23.71726841066015 3 19.035303701469566 26.250371725468586 29.70435316949695 25.0099714035649 4 12.449144269454962 16.62255878386385 37.15642404509725 33.77187290158393 5 12.72950471940771 39.208080990307494 33.60596011089234 14.45645417939246 6 31.2544140969163 37.08066960352423 17.16285462555066 14.502061674008809 7 28.35905668062412 33.94833532289366 20.39118467394935 17.301423322532862 8 27.63689560939035 34.365367838463676 19.118664706703104 18.879071845442873 9 28.1858451356307 15.194556450192168 19.41280017814434 37.206798236032796 10-11 25.2783152957492 26.948277538733006 26.492487354429723 21.280919811088076 12-13 26.73067084591783 24.361379322008577 27.532884120207967 21.375065711865627 14-15 23.15693191475568 24.891302356325323 26.353875837341818 25.597889891577186 16-17 21.99547028555241 28.76051566021222 26.766750712084075 22.47726334215129 18-19 22.09814287345128 26.143527398853056 30.198002359285 21.560327368410668 20-21 23.009441368103424 25.19008874801455 30.41152187976103 21.388948004120998 22-23 22.680142120847822 25.275285150739148 30.344013067676528 21.70055966073651 24-25 22.259069957217946 25.549292380200033 30.571585416471407 21.62005224611061 26-27 21.92986412266027 25.89456222860823 30.297010585776356 21.878563062955138 28-29 21.888294367645265 25.802832702285794 30.481322613629075 21.827550316439865 30-31 21.8452140588409 25.622366504366873 30.59270080811626 21.93971862867597 32-33 21.73741468027742 25.79210104617518 30.32879187413764 22.141692399409756 34-35 22.309971150200763 25.71543133057191 30.136835711175035 21.837761808052296 36-37 22.56791076960371 25.40745052386496 29.82217906400539 22.20245964252594 38-39 22.179956788722787 25.590913511205194 29.926698678152093 22.302431021919926 40-41 22.421663704641194 25.5436291553506 29.842347897290765 22.192359242717437 42-43 22.352745522784577 25.524814068892816 29.733826424837606 22.388613983485005 44-45 22.367854502710667 25.762866422883913 29.509176384033243 22.360102690372177 46-47 22.8678059791 26.158685818541088 28.62570056016625 22.34780764219266 48-49 22.86476956099541 26.331889668896757 28.18598607260791 22.61735469749992 50-51 22.87509249145555 26.450583136605477 28.073288467636797 22.601035904302176 52-53 23.162005645935306 26.380231052080443 28.042018950385955 22.415744351598295 54-55 22.58504839522355 26.546374498342196 28.099602197252377 22.76897490918188 56-57 22.745812398260835 26.716018237296257 27.960777128682867 22.57739223576004 58-59 22.861268376834513 26.10817720687619 28.076688357356584 22.953866058932718 60-61 22.52407029095755 26.37390747339989 28.440185952378773 22.661836283263792 62-63 22.57555534570896 26.358431769396756 28.600533041677885 22.465479843216404 64-65 22.300670802306055 26.26502152500939 28.637035982599592 22.797271690084965 66-67 22.31499013792807 26.52555576774415 28.340050159505537 22.819403934822237 68-69 22.490685755318825 26.169451250578728 28.56955610850258 22.770306885599865 70-71 22.41313577984884 26.313208012543825 28.384136995472243 22.88951921213509 72-73 22.63262487315368 26.459578306460706 27.89336452813169 23.014432292253918 74-75 22.355141451397177 26.846591509674617 27.926027818140565 22.87223922078764 76-77 22.586391817670595 26.53062806453295 27.942127805910683 22.940852311885774 78-79 22.5879011884586 26.567845729406336 27.825857166514574 23.018395915620495 80-81 22.564653304384777 26.869377035225206 27.69224047597404 22.873729184415982 82-83 22.76679947740127 26.672196140831055 27.747413432253136 22.81359094951454 84-85 22.563728177670793 26.621092346135576 27.757537661883735 23.057641814309896 86-87 22.583229858310865 26.47865924559309 27.762783099909655 23.175327796186387 88-89 22.568442179325274 26.702386455890313 27.584340609778096 23.144830755006314 90-91 22.56054934151182 26.716903639725782 27.656785585423055 23.065761433339347 92-93 22.464459051005743 26.512952458673272 27.726978985861138 23.29560950445984 94-95 22.317577209703845 26.27818396434171 27.90852874332728 23.495710082627156 96-97 22.584057375576197 26.227536132814567 27.76284160476279 23.425564886846452 98-99 22.36752809550958 26.343147528645193 27.469152550495103 23.820171825350123 100-101 22.099882029099486 26.269808047109432 27.92161207165076 23.70869785214032 102-103 22.209694252551184 25.87518242877086 27.896750424756895 24.01837289392106 104-105 22.04259475366584 25.993835303668373 27.90032472474504 24.06324521792075 106-107 22.21765743012697 25.9640441583737 27.826173969785934 23.9921244417134 108-109 22.25672345274784 25.813999214912986 27.52582717796457 24.403450154374603 110-111 22.04201431953997 26.100955575600405 27.674202277596123 24.1828278272635 112-113 22.01403369807042 26.07953556450857 27.888609899979777 24.017820837441235 114-115 21.949736236447148 26.247257014081015 27.469603417439824 24.333403332032017 116-117 21.712272730155533 26.11479465820293 27.786801665594368 24.386130946047174 118-119 21.480768959733908 26.350964723126573 27.52075317463673 24.647513142502785 120-121 21.523995963559283 26.45031400802778 27.689645063816354 24.33604496459658 122-123 21.391816145867836 26.46102500426345 27.52216642542342 24.624992424445296 124-125 21.37631403821426 26.363737620970472 27.337000045117193 24.922948295698074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 279.0 1 195.5 2 260.0 3 388.5 4 226.5 5 134.5 6 362.0 7 986.5 8 1698.0 9 1875.5 10 1324.5 11 717.0 12 604.5 13 637.0 14 681.5 15 785.0 16 843.5 17 903.0 18 1027.5 19 1126.5 20 1281.0 21 1483.5 22 1714.0 23 2004.0 24 2334.0 25 2804.0 26 3498.5 27 4426.0 28 5297.0 29 6496.5 30 8036.0 31 9221.0 32 11059.0 33 12422.5 34 13217.0 35 14244.0 36 15816.5 37 17748.5 38 18765.0 39 19519.5 40 20289.5 41 21165.0 42 21832.5 43 22779.0 44 24134.0 45 25412.5 46 25679.5 47 25837.5 48 26042.0 49 25139.5 50 24607.5 51 23956.5 52 23245.5 53 23571.5 54 24242.0 55 23415.5 56 21181.5 57 19434.5 58 18648.0 59 17181.0 60 14125.0 61 11491.0 62 9761.0 63 7680.5 64 5853.0 65 4627.5 66 3281.0 67 2573.0 68 1919.0 69 1095.5 70 788.5 71 601.0 72 439.0 73 345.0 74 297.0 75 224.0 76 146.5 77 77.0 78 41.0 79 25.0 80 17.0 81 10.0 82 8.5 83 5.5 84 2.5 85 1.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02480490798929443 2 0.0022549916353904026 3 0.0011274958176952013 4 0.0942868377547612 5 0.002818739544238003 6 0.022831790308327826 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014798382607249516 26-27 0.0 28-29 3.5234244302975037E-4 30-31 0.006694506417565258 32-33 0.0 34-35 0.0 36-37 4.2281093163570047E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.003593892918903454 46-47 0.0021845231467844525 48-49 0.0 50-51 0.0016912437265428019 52-53 0.0 54-55 0.002043586169572552 56-57 2.8187395442380033E-4 58-59 0.0022549916353904026 60-61 7.046848860595008E-5 62-63 0.0033824874530856038 64-65 0.0019026491923606521 66-67 7.046848860595008E-5 68-69 7.751533746654509E-4 70-71 0.0034529559416915535 72-73 0.0023959286126023025 74-75 0.0 76-77 0.0011274958176952013 78-79 6.342163974535507E-4 80-81 3.5234244302975037E-4 82-83 8.456218632714009E-4 84-85 0.0 86-87 0.003664361407509404 88-89 0.004650920247992705 90-91 0.004650920247992705 92-93 0.005426073622658156 94-95 0.0034529559416915535 96-97 0.005144199668234355 98-99 0.005285136645446256 100-101 0.005637479088476006 102-103 0.0019026491923606521 104-105 0.001832180703754702 106-107 0.0 108-109 0.00866762409853186 110-111 0.0023959286126023025 112-113 0.004298577804962955 114-115 0.0 116-117 7.046848860595008E-5 118-119 9.86558840483301E-4 120-121 1.4093697721190017E-4 122-123 0.002536865589814203 124-125 0.03847579477884874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 709537.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.62923393393169 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64394062062311 39.2845341267328 2 13.643625416190908 14.361071074712948 3 4.680759039547003 7.390342874420537 4 2.218334626693109 4.669970080478906 5 1.2557519147576028 3.3044630642380257 6 0.7582368250293214 2.3943253945073875 7 0.5019499005319299 1.8492067116746076 8 0.37054943530979895 1.5601386332004554 9 0.27670965104284645 1.3106715260859512 >10 1.5131389770333248 14.438420917911083 >50 0.09517871491089332 3.3687086595784614 >100 0.03807136360827633 3.823899127515704 >500 0.002949190138600682 0.9671219416848084 >1k 8.043245832547315E-4 1.2771258672584187 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3878 0.5465535976277488 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3112 0.4385958730834333 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2061 0.2904711100337262 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 853 0.12021924156175082 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 763 0.10753491361267982 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.046848860595008E-5 0.0 20-21 0.0 0.0 0.0 1.4093697721190017E-4 0.0 22-23 0.0 0.0 0.0 2.8187395442380033E-4 0.0 24-25 1.4093697721190017E-4 0.0 0.0 5.637479088476007E-4 0.0 26-27 1.4093697721190017E-4 0.0 0.0 7.751533746654508E-4 0.0 28-29 2.1140546581785023E-4 0.0 0.0 0.0018321807037547022 0.0 30-31 2.8187395442380033E-4 0.0 0.0 0.004791857225204605 0.0 32-33 2.8187395442380033E-4 0.0 0.0 0.011063552711134163 0.0 34-35 2.8187395442380033E-4 0.0 0.0 0.01888555494639462 0.0 36-37 2.8187395442380033E-4 0.0 0.0 0.03551611825739884 0.0 38-39 2.8187395442380033E-4 0.0 0.0 0.06123711659857062 0.0 40-41 2.8187395442380033E-4 0.0 0.0 0.09710557729899921 0.0 42-43 3.523424430297504E-4 0.0 0.0 0.1301552984551898 0.0 44-45 4.2281093163570047E-4 0.0 0.0 0.1663056331100422 0.0 46-47 4.2281093163570047E-4 0.0 0.0 0.21027797000015502 0.0 48-49 4.2281093163570047E-4 0.0 0.0 0.26024012842177363 0.0 50-51 4.2281093163570047E-4 0.0 0.0 0.3226752093266454 0.0 52-53 4.2281093163570047E-4 0.0 0.0 0.37947281114304116 0.0 54-55 4.2281093163570047E-4 0.0 0.0 0.438948215526463 0.0 56-57 4.2281093163570047E-4 0.0 0.0 0.5113193533247737 0.0 58-59 4.2281093163570047E-4 0.0 0.0 0.5975023148898506 0.0 60-61 4.2281093163570047E-4 0.0 0.0 0.6936213333483666 0.0 62-63 4.2281093163570047E-4 0.0 0.0 0.7964348582244478 0.0 64-65 4.2281093163570047E-4 0.0 0.0 0.8897351371387257 0.0 66-67 5.637479088476007E-4 0.0 0.0 0.9965653658653459 0.0 68-69 5.637479088476007E-4 0.0 0.0 1.1213650591864837 0.0 70-71 5.637479088476007E-4 0.0 0.0 1.2642751540793502 0.0 72-73 5.637479088476007E-4 0.0 0.0 1.41740317982008 0.0 74-75 5.637479088476007E-4 0.0 0.0 1.5620749869280954 0.0 76-77 5.637479088476007E-4 0.0 0.0 1.7158372290662784 0.0 78-79 5.637479088476007E-4 0.0 0.0 1.9100483836642768 0.0 80-81 5.637479088476007E-4 0.0 0.0 2.1388595661677967 0.0 82-83 5.637479088476007E-4 0.0 0.0 2.380707419063417 0.0 84-85 5.637479088476007E-4 0.0 0.0 2.6096595385441494 0.0 86-87 5.637479088476007E-4 0.0 0.0 2.8469974081689893 0.0 88-89 5.637479088476007E-4 0.0 0.0 3.1197104590740157 0.0 90-91 5.637479088476007E-4 0.0 0.0 3.4378052166412747 0.0 92-93 5.637479088476007E-4 0.0 0.0 3.7852853339572143 0.0 94-95 5.637479088476007E-4 0.0 0.0 4.114161770281184 0.0 96-97 5.637479088476007E-4 0.0 0.0 4.439232908220431 0.0 98-99 5.637479088476007E-4 0.0 0.0 4.789954576012244 0.0 100-101 6.342163974535507E-4 0.0 0.0 5.2145976883517 0.0 102-103 7.046848860595007E-4 0.0 0.0 5.676307225697885 0.0 104-105 9.86558840483301E-4 0.0 0.0 6.134845681056802 0.0 106-107 9.86558840483301E-4 0.0 0.0 6.585139323248823 0.0 108-109 9.86558840483301E-4 0.0 0.0 7.0497380686278515 0.0 110-111 0.0010570273290892512 0.0 0.0 7.592697773336697 0.0 112-113 0.0011274958176952013 0.0 0.0 8.18561963646716 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1885 0.0 82.5242 1 GTATCAA 3875 0.0 62.44627 1 GCGTATC 35 0.0077430317 51.08641 1 ATCAACG 5025 0.0 48.022915 3 TCAACGC 5085 0.0 47.452923 4 TATCAAC 5150 0.0 46.973007 2 CAACGCA 5245 0.0 45.953472 5 AACGCAG 5395 0.0 44.672657 6 GTGGTAT 745 0.0 42.400578 1 TGGTATC 735 0.0 42.154655 2 GTACATG 4870 0.0 39.897057 1 ACGCAGA 6170 0.0 38.956738 7 CGCAGAG 6190 0.0 38.638634 8 TACATGG 5000 0.0 38.25292 2 ACATGGG 5080 0.0 36.59489 3 GCAGAGT 6800 0.0 34.647556 9 CATGGGA 3300 0.0 32.136974 4 CATGGGG 1895 0.0 31.126112 4 ATGGGAT 1325 0.0 30.542307 5 GAGTACT 4280 0.0 29.956417 12-13 >>END_MODULE