##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940129_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156559 Sequences flagged as poor quality 0 Sequence length 125 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.917091958941995 31.0 22.0 34.0 7.0 34.0 2 25.800420288836797 31.0 22.0 34.0 7.0 34.0 3 26.414489106343296 31.0 23.0 34.0 7.0 34.0 4 28.01105014722884 35.0 20.0 37.0 7.0 37.0 5 29.792103935257636 35.0 29.0 37.0 7.0 37.0 6 28.43162641560051 35.0 21.0 37.0 8.0 37.0 7 28.73783685383785 35.0 25.0 37.0 8.0 37.0 8 27.802291787760524 35.0 17.0 37.0 7.0 37.0 9 28.125409589994828 37.0 13.0 39.0 7.0 39.0 10-11 29.005617690455356 37.0 20.0 39.0 7.0 39.0 12-13 28.85311607764485 37.0 18.5 39.0 7.0 39.0 14-15 29.75765366411385 38.0 17.5 41.0 7.0 41.0 16-17 30.216841574103054 38.0 23.0 41.0 7.0 41.0 18-19 30.274624901794212 37.0 24.5 41.0 2.0 41.0 20-21 30.420486845214903 37.5 25.0 41.0 2.0 41.0 22-23 30.720814517210762 37.0 27.0 41.0 2.0 41.0 24-25 30.967507457252538 37.0 27.5 41.0 2.0 41.0 26-27 31.007422122011512 37.0 28.0 41.0 2.0 41.0 28-29 30.85591055129376 36.5 28.0 41.0 2.0 41.0 30-31 30.60198391660652 36.0 28.0 41.0 2.0 41.0 32-33 30.403244144380075 36.0 28.0 41.0 2.0 41.0 34-35 30.138685735090284 35.0 27.0 41.0 2.0 41.0 36-37 29.779996678568466 35.0 26.5 41.0 2.0 41.0 38-39 29.437454889211097 35.0 25.0 41.0 2.0 41.0 40-41 29.193495742819003 35.0 25.0 40.0 2.0 41.0 42-43 28.935717525022515 35.0 25.0 40.5 2.0 41.0 44-45 28.845380974584664 35.0 25.0 41.0 2.0 41.0 46-47 28.656809892756087 35.0 25.0 41.0 2.0 41.0 48-49 28.425721293569836 35.0 25.0 40.5 2.0 41.0 50-51 28.219881961433067 35.0 25.0 40.0 2.0 41.0 52-53 27.966744166735864 35.0 23.5 40.0 2.0 41.0 54-55 27.730970432871953 35.0 22.5 40.0 2.0 41.0 56-57 27.46499722149477 34.5 16.0 40.0 2.0 41.0 58-59 27.112270773318684 34.0 2.0 40.0 2.0 41.0 60-61 26.76962039869953 34.0 2.0 40.0 2.0 41.0 62-63 26.399395754955002 34.0 2.0 39.0 2.0 41.0 64-65 26.058297510842557 33.0 2.0 39.0 2.0 41.0 66-67 25.705254887933624 33.0 2.0 38.5 2.0 41.0 68-69 25.370441814268105 33.0 2.0 37.5 2.0 41.0 70-71 25.195692997528088 33.0 2.0 37.0 2.0 41.0 72-73 24.922607451503907 33.0 2.0 37.0 2.0 40.0 74-75 24.585134038924622 33.0 2.0 36.0 2.0 39.0 76-77 24.247443455821767 33.0 2.0 36.0 2.0 39.0 78-79 23.948594459596702 33.0 2.0 35.5 2.0 39.0 80-81 23.631202933079543 33.0 2.0 35.0 2.0 37.0 82-83 23.322319381191754 32.5 2.0 35.0 2.0 37.0 84-85 23.039381319502553 32.0 2.0 35.0 2.0 36.5 86-87 22.778102823855544 32.0 2.0 35.0 2.0 36.0 88-89 22.550239845681183 32.0 2.0 35.0 2.0 36.0 90-91 22.340909816746404 31.5 2.0 35.0 2.0 36.0 92-93 22.155149176987592 31.0 2.0 35.0 2.0 35.0 94-95 21.990307168543488 31.0 2.0 35.0 2.0 35.0 96-97 21.835624269444743 31.0 2.0 35.0 2.0 35.0 98-99 21.724503222427327 30.5 2.0 35.0 2.0 35.0 100-101 21.612874379626852 30.0 2.0 35.0 2.0 35.0 102-103 20.97769211607126 29.0 2.0 34.5 2.0 35.0 104-105 21.346505151412565 30.0 2.0 35.0 2.0 35.0 106-107 21.34832555138957 30.0 2.0 35.0 2.0 35.0 108-109 21.2783647059575 30.0 2.0 35.0 2.0 35.0 110-111 21.15165528650541 29.5 2.0 35.0 2.0 35.0 112-113 21.01103737249216 29.0 2.0 35.0 2.0 35.0 114-115 20.839609987289137 29.0 2.0 35.0 2.0 35.0 116-117 20.68914913866338 29.0 2.0 35.0 2.0 35.0 118-119 20.49908341264316 28.0 2.0 35.0 2.0 35.0 120-121 20.27993280488506 28.0 2.0 35.0 2.0 35.0 122-123 19.99980518526562 28.0 2.0 35.0 2.0 35.0 124-125 18.4767020739785 25.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9869256692971966 1101 2 0.7197790372907846 1101 3 0.6106306506292896 1101 4 1.2856922895001688 1101 5 1.2171620211638832 1101 6 0.7484301119245167 1101 7 0.7391323742391833 1101 8 0.8281347615349901 1101 9 1.193562024953149 1101 10-11 0.1668094172318071 1101 12-13 0.6791551632075858 1101 14-15 0.6549052774578854 1101 16-17 0.680535238620017 1101 18-19 0.2196671348644159 1101 20-21 -0.05831034391034251 1101 22-23 -0.489689458360111 1101 24-25 -0.6211404586278704 1101 26-27 -0.25156789197896146 1101 28-29 0.055716538777229374 1101 30-31 0.1602840430749879 1101 32-33 0.09899182996987577 1101 34-35 0.17710468331839735 1101 36-37 0.11943252873262722 1101 38-39 -0.029822892236055054 1101 40-41 0.17130483413543374 1101 42-43 0.44751373554190366 1101 44-45 0.35508802401596284 1101 46-47 0.35986666866636696 1101 48-49 0.5492613092132359 1101 50-51 0.6987530481917332 1101 52-53 0.4630024212581496 1101 54-55 0.12017789455065042 1101 56-57 0.45198949138446665 1101 58-59 0.1743000792433449 1101 60-61 0.2960419256141549 1101 62-63 0.11947031382196016 1101 64-65 0.11355502157122999 1101 66-67 0.36388143926474825 1101 68-69 0.19905265077387924 1101 70-71 -0.12313164794857911 1101 72-73 -0.21560919488405972 1101 74-75 -0.32595307305742693 1101 76-77 -0.4712750007563393 1101 78-79 -0.3645441585302436 1101 80-81 -0.33199685587688776 1101 82-83 -0.3069401397332392 1101 84-85 -0.3724555658317463 1101 86-87 -0.0542231034211369 1101 88-89 0.010685968103448573 1101 90-91 0.014511616946006 1101 92-93 0.0444505482646278 1101 94-95 0.0995826890122089 1101 96-97 -0.06181100206619661 1101 98-99 -0.34076935978673717 1101 100-101 -0.4002014423763711 1101 102-103 -0.1117317476914188 1101 104-105 -0.1443448784752306 1101 106-107 -0.07101913634942036 1101 108-109 -0.1055605960234729 1101 110-111 0.048070622556561915 1101 112-113 0.10279377381643329 1101 114-115 0.277187782830719 1101 116-117 0.259139253754892 1101 118-119 0.09398049698922151 1101 120-121 0.013989537105075556 1101 122-123 -0.20747448700353033 1101 124-125 -0.4416327066342589 1102 1 0.4430094726815632 1102 2 0.6242811964263026 1102 3 0.720674246571356 1102 4 0.6676629913741756 1102 5 0.2444353119343532 1102 6 0.9416985678843517 1102 7 0.547372213905291 1102 8 1.0760117837875143 1102 9 1.0917624009939786 1102 10-11 1.17802865729875 1102 12-13 1.2006948644410826 1102 14-15 1.2001128230683342 1102 16-17 1.6565471396923535 1102 18-19 1.519084478197957 1102 20-21 1.4248401344053256 1102 22-23 1.2499849150773557 1102 24-25 1.155704104276321 1102 26-27 1.1959516848718046 1102 28-29 1.532162333111824 1102 30-31 1.5073676605050963 1102 32-33 1.4372632818593765 1102 34-35 1.5616684139739938 1102 36-37 1.634629123703597 1102 38-39 1.7664435595501402 1102 40-41 1.8462154727849942 1102 42-43 1.5930745942373328 1102 44-45 1.371080089967741 1102 46-47 1.3216530692118305 1102 48-49 1.2388623340277967 1102 50-51 0.7257370767873681 1102 52-53 0.9902963736125407 1102 54-55 1.4823444682401927 1102 56-57 1.4627851689811102 1102 58-59 1.2706966990082194 1102 60-61 1.5001473823384686 1102 62-63 1.7112490695813776 1102 64-65 1.670085115168206 1102 66-67 1.5954461410147864 1102 68-69 1.4858044431662805 1102 70-71 1.6247660358878626 1102 72-73 1.5041990656171897 1102 74-75 1.5634661298186643 1102 76-77 1.6167330159382232 1102 78-79 1.6370881065991973 1102 80-81 1.5185475768593264 1102 82-83 1.5340276488574318 1102 84-85 1.6709957451457491 1102 86-87 1.469238125116643 1102 88-89 1.265329659088799 1102 90-91 1.3997778414547746 1102 92-93 1.4374028819905362 1102 94-95 1.2860844762392176 1102 96-97 1.0758261391970514 1102 98-99 0.9464679798277196 1102 100-101 1.0523388993662266 1102 102-103 1.234804337764892 1102 104-105 1.104875146731903 1102 106-107 1.2632749606360534 1102 108-109 1.2621949361156233 1102 110-111 1.1057577648528927 1102 112-113 1.260028427424981 1102 114-115 1.2244594206744743 1102 116-117 1.1915345511783961 1102 118-119 1.2559879764825972 1102 120-121 1.3243058659548481 1102 122-123 1.5753412736526826 1102 124-125 1.6649604047936606 1103 1 -1.4863608930341599 1103 2 -1.112198213736658 1103 3 -0.29235411420113167 1103 4 -0.9850536941438186 1103 5 -0.6041257035237351 1103 6 -0.23556673310848453 1103 7 -1.0016621792914755 1103 8 -1.00705972557531 1103 9 -1.0171773166550544 1103 10-11 -0.5846331499150246 1103 12-13 -0.9684329475483615 1103 14-15 -0.8182567467025201 1103 16-17 -1.135428479259513 1103 18-19 -1.4702016849288526 1103 20-21 -1.598608820393384 1103 22-23 -1.4060516983866798 1103 24-25 -1.0434182617745371 1103 26-27 -1.353585410348341 1103 28-29 -1.4591346381084875 1103 30-31 -1.109133533412205 1103 32-33 -1.19402572991223 1103 34-35 -1.2239082271386543 1103 36-37 -1.2097322352675377 1103 38-39 -1.1338433995215347 1103 40-41 -1.077740373220621 1103 42-43 -1.0441525851853974 1103 44-45 -0.9602633811897334 1103 46-47 -0.7951937323465437 1103 48-49 -0.8978307244950976 1103 50-51 -0.9119613857822806 1103 52-53 -0.9798138019142506 1103 54-55 -0.8971536675731393 1103 56-57 -0.8723390403104965 1103 58-59 -0.6838820785880273 1103 60-61 -0.5099276040466876 1103 62-63 -0.556450230824975 1103 64-65 -0.18887554839846388 1103 66-67 -0.2309855124559519 1103 68-69 -0.3975163226164682 1103 70-71 -0.25646013813081936 1103 72-73 -0.4864485821562603 1103 74-75 -0.3595576228519377 1103 76-77 -0.3008133792693535 1103 78-79 -0.11208452965399474 1103 80-81 -0.12257554324456521 1103 82-83 0.010756734358672304 1103 84-85 -0.17624567750097597 1103 86-87 -0.6094661604181084 1103 88-89 -0.4851835158933966 1103 90-91 -0.27305739795706074 1103 92-93 -0.15814192132729943 1103 94-95 -0.3017208105103961 1103 96-97 -0.43124310800666876 1103 98-99 -0.3276617172943119 1103 100-101 -0.561167028328466 1103 102-103 -0.42669916020335563 1103 104-105 -0.5165064459956739 1103 106-107 -0.6165897287359883 1103 108-109 -0.49635099828176266 1103 110-111 -0.46362558733176584 1103 112-113 -0.5088962091633498 1103 114-115 -0.23116665280380388 1103 116-117 -0.2257604930174928 1103 118-119 -0.22382015891181695 1103 120-121 -0.06197874717405227 1103 122-123 0.00997953765502757 1103 124-125 0.450641775494514 1104 1 0.8324158653756264 1104 2 0.7856815008403046 1104 3 0.9475235616398479 1104 4 1.0443448787409935 1104 5 0.012958904019590989 1104 6 1.3233119621004867 1104 7 1.2813143752447118 1104 8 0.7011563804389596 1104 9 0.5117319595936713 1104 10-11 0.7583026299014897 1104 12-13 0.9038912114730486 1104 14-15 0.6180123664473278 1104 16-17 0.2094542934214232 1104 18-19 0.46346803984179274 1104 20-21 -0.5966210528092866 1104 22-23 -0.2518111275406021 1104 24-25 -0.47562009663691995 1104 26-27 -0.7538504460262203 1104 28-29 -0.616209051971893 1104 30-31 -0.5531498433000763 1104 32-33 -0.33728770748613 1104 34-35 -0.24706826791337377 1104 36-37 -0.27459462058863693 1104 38-39 -0.6021104739353973 1104 40-41 -0.38474343132459765 1104 42-43 -0.3869558471629695 1104 44-45 -0.7782654199257131 1104 46-47 -0.6679664132843648 1104 48-49 -0.5353842413146666 1104 50-51 -0.5048869719188751 1104 52-53 -0.2523520282139451 1104 54-55 -0.11595081334276358 1104 56-57 -0.04902609433700178 1104 58-59 -0.2000186738989349 1104 60-61 -0.4701722523647298 1104 62-63 -0.7791923307230348 1104 64-65 -0.5542680050753255 1104 66-67 -0.5957867356975406 1104 68-69 -0.603601949527782 1104 70-71 -0.48348358359463006 1104 72-73 -0.6857248308820516 1104 74-75 -0.7906891213228846 1104 76-77 -0.7092639399217475 1104 78-79 -0.425499412426678 1104 80-81 -0.752167796047825 1104 82-83 -0.4529129904119742 1104 84-85 -0.5214852289790599 1104 86-87 -0.1532276283080165 1104 88-89 -0.1250204170147029 1104 90-91 0.05119336656893125 1104 92-93 -0.27160777249957135 1104 94-95 -0.20791856790081198 1104 96-97 -0.1497446370586566 1104 98-99 -0.08436915868065142 1104 100-101 -0.21225775207973996 1104 102-103 -0.16632199404637404 1104 104-105 -0.16282652754511417 1104 106-107 -0.15955608959225742 1104 108-109 -0.13256914303201484 1104 110-111 -0.30644923666917734 1104 112-113 0.08782142590290931 1104 114-115 -0.02461211661623608 1104 116-117 0.09963196365936611 1104 118-119 0.2697170480198885 1104 120-121 0.08768486138863807 1104 122-123 -0.2935780566365125 1104 124-125 -0.2202450746583935 1105 1 1.337229019336764 1105 2 1.4279136881845993 1105 3 1.202112500354346 1105 4 1.2289587302606755 1105 5 0.9751313006314497 1105 6 1.6841888928428865 1105 7 2.183058271607443 1105 8 2.0376187621031363 1105 9 1.7778405794242644 1105 10-11 1.6535492666727905 1105 12-13 2.057104963341505 1105 14-15 2.10154001966108 1105 16-17 2.170460770750772 1105 18-19 1.6453514479135407 1105 20-21 1.6039768545150785 1105 22-23 1.4722446771430029 1105 24-25 1.427608602914649 1105 26-27 1.3089397881541451 1105 28-29 1.4039603551033473 1105 30-31 1.7925203111593149 1105 32-33 2.04257278080485 1105 34-35 1.824102633930174 1105 36-37 1.7569748861378187 1105 38-39 2.0491800093730212 1105 40-41 1.7865271217396774 1105 42-43 1.764205089558505 1105 44-45 1.9985756363772254 1105 46-47 2.200085405350414 1105 48-49 1.8451239799110404 1105 50-51 2.017544517866874 1105 52-53 2.2882159837442018 1105 54-55 2.0402923356358116 1105 56-57 1.6431811802021095 1105 58-59 1.8397222329271727 1105 60-61 1.943131185602386 1105 62-63 1.8992929707219055 1105 64-65 2.2732855574558144 1105 66-67 2.2283986155730098 1105 68-69 1.8479376618324537 1105 70-71 1.8419374428129416 1105 72-73 1.6963279842649364 1105 74-75 1.2153796380194137 1105 76-77 1.4221162294853258 1105 78-79 1.501565859122902 1105 80-81 1.6354056967274566 1105 82-83 1.582423332457985 1105 84-85 1.7866662408764462 1105 86-87 1.5527813403018484 1105 88-89 1.5722690697815125 1105 90-91 1.6585775220248387 1105 92-93 1.6518202294934525 1105 94-95 1.8065607544708868 1105 96-97 1.939791984764959 1105 98-99 2.134993073506692 1105 100-101 1.9647925717867274 1105 102-103 2.0636431280263743 1105 104-105 1.962923347890861 1105 106-107 1.997544059884575 1105 108-109 2.1879889038040545 1105 110-111 1.807561724965101 1105 112-113 1.9461373202726158 1105 114-115 1.826728192302543 1105 116-117 1.767235351800874 1105 118-119 1.6120090260966187 1105 120-121 1.9648448015979056 1105 122-123 1.6919127256255706 1105 124-125 1.9290224888692613 1106 1 -0.31869524573548347 1106 2 -0.49431849915969295 1106 3 -0.2924764383601506 1106 4 -0.22232178792567225 1106 5 -0.09815220709152328 1106 6 0.10553418432270689 1106 7 0.023536597466932818 1106 8 0.456711935994516 1106 9 0.23173195959367376 1106 10-11 0.22274707434593566 1106 12-13 0.23278010036193564 1106 14-15 -0.1775431891082313 1106 16-17 0.3338987378658693 1106 18-19 0.3568013731751236 1106 20-21 0.5633789471907136 1106 22-23 -0.01181112754060365 1106 24-25 0.13549101447419076 1106 26-27 -0.26273933491510704 1106 28-29 -0.3473201630830012 1106 30-31 -0.6242609544111843 1106 32-33 -0.6706210408194657 1106 34-35 -0.7181793790244839 1106 36-37 -0.8257057316997489 1106 38-39 -1.2554438072687333 1106 40-41 -1.211410097991262 1106 42-43 -0.7447336249407499 1106 44-45 -1.0693765310368235 1106 46-47 -0.9479664132843659 1106 48-49 -0.9153842413146691 1106 50-51 -0.8404425274744298 1106 52-53 -0.8790186948806102 1106 54-55 -0.7759508133427602 1106 56-57 -0.8556927610036702 1106 58-59 -0.8711297850100479 1106 60-61 -1.1479500301425034 1106 62-63 -0.8058589973897057 1106 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-2.7571772060178397 2216 112-113 -2.648883554940003 2216 114-115 -2.4835393196813733 2216 116-117 -2.5028967719728143 2216 118-119 -2.509823181865169 2216 120-121 -2.320054602212899 2216 122-123 -2.495723650506246 2216 124-125 -2.195953886918929 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10747.0 3 2634.0 4 3230.0 5 2823.0 6 4201.0 7 3344.0 8 2502.0 9 2411.0 10 2688.0 11 2886.0 12 2634.0 13 2353.0 14 1984.0 15 1838.0 16 1716.0 17 1478.0 18 1368.0 19 1073.0 20 908.0 21 845.0 22 809.0 23 1700.0 24 3682.0 25 2671.0 26 4253.0 27 2533.0 28 1850.0 29 2046.0 30 2054.0 31 2167.0 32 2753.0 33 3485.0 34 5129.0 35 9197.0 36 15559.0 37 27136.0 38 15774.0 39 98.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.85901924207191 24.138834231978127 11.558658932358878 15.443487593591087 2 13.075252331672417 34.254503641241854 33.9849239810911 18.685320045994633 3 16.34023252842724 45.380733358885905 23.390187811422 14.888846301264852 4 9.615814660763004 28.835943340169827 29.654405704318492 31.893836294748677 5 9.423896445753495 54.86880019422811 22.513209427729894 13.194093932288506 6 20.886921076674398 45.727355784281784 16.85162823682748 16.534094902216346 7 22.962726372608515 49.839343319812194 14.929892363218244 12.268037944361046 8 22.18588519344145 47.03466424798319 15.145727808685544 15.633722749889817 9 26.040934701230338 32.495432535677324 13.459007806211751 28.00462495688058 10-11 19.513410279830605 44.87796932785723 18.126073876302225 17.48254651600994 12-13 21.664979288362836 43.49411866155236 19.302862581401364 15.53803946868343 14-15 20.586206015383436 41.84415966064447 19.74420565762911 17.825428666342987 16-17 17.56575060281366 44.599907382271695 20.242402950992446 17.5919390639222 18-19 17.94753046988783 42.120308148299564 23.704870071799064 16.22729131001354 20-21 22.78567527602209 39.148986455196116 22.293514399138957 15.771823869642839 22-23 21.53820604372792 41.00466916625681 21.776454882823728 15.680669907191538 24-25 18.779224263484682 40.647916294044 23.832307024043743 16.740552418427576 26-27 18.440332398648433 40.79260853735652 23.53553612376165 17.231522940233397 28-29 18.862103326690175 40.54148142470233 23.443009351525372 17.15340589708212 30-31 18.413244889290585 41.07921320145505 23.370177920713346 17.137363988541026 32-33 17.445930581637477 41.52646303606331 23.15180316559997 17.875803216699243 34-35 18.130545034140482 40.90470685173002 23.904087277001 17.060660837128495 36-37 17.919137913963866 41.22695232528801 22.61251161765692 18.241398143091207 38-39 18.147529517720212 41.490880405726934 22.985018379711097 17.376571696841754 40-41 17.864591817505264 41.473347323233774 23.22483175387996 17.437229105381004 42-43 17.15320540664844 41.66070470398856 23.424764289546975 17.76132559981603 44-45 17.476007217795377 41.51925011577216 23.431965891126264 17.5727767753062 46-47 16.997144518049584 41.68971707092711 23.21436556557068 18.09877284545263 48-49 16.620995816166843 42.0567851553767 22.66072626233592 18.66149276612053 50-51 17.427576735124088 41.976428502986366 22.457759749592768 18.138235012296782 52-53 17.07164392661863 42.137436762225974 22.38074250089427 18.410176810261127 54-55 17.027188441159844 41.68873019472328 22.781058474470715 18.503022889646164 56-57 17.261324909851066 42.33598323820718 21.642770678360744 18.759921173581013 58-59 16.76265312315334 41.809528372702154 22.128312011882517 19.29950649226199 60-61 16.38545828502809 42.105246348762265 21.780635106175726 19.728660260033916 62-63 16.806558968757386 42.02358367028853 21.840126733482805 19.32973062747127 64-65 16.388013273163363 42.189880330743215 21.873892173113223 19.5482142229802 66-67 16.10850999955288 42.365400461173884 21.288412527066818 20.23767701220642 68-69 16.555100632988204 41.83917883992616 21.851187092406057 19.754533434679576 70-71 16.09720731769636 41.9129107261485 22.03548980530431 19.954392150850836 72-73 16.044430534175614 41.65458389488947 22.037059511110826 20.26392605982409 74-75 15.936071746816522 42.143589448772126 22.292806474588073 19.627532329823282 76-77 15.859022394281169 42.01059823490518 22.6137202104315 19.51665916038215 78-79 16.369808982699535 41.50705975159128 22.68318466496121 19.439946600747973 80-81 16.56712699771325 41.755560381721324 22.51108243800861 19.166230182556816 82-83 16.477332609424653 40.97983169123165 22.4128515082318 20.12998419111189 84-85 17.35703472812167 40.15099738756635 23.009216972515155 19.48275091179683 86-87 17.0710987052639 40.46194037954228 22.493724330435562 19.973236584758265 88-89 17.67649829929257 39.69690678845755 22.830999185576722 19.79559572667316 90-91 17.635730095208284 39.84311871812151 22.410516667039282 20.11063451963092 92-93 17.530192253024016 39.921169552538196 22.31368002120884 20.23495817322895 94-95 17.287093044794613 39.645437183426054 22.654398661207594 20.413071110571735 96-97 17.140293054331302 40.3697032409714 22.138440705680964 20.35156299901634 98-99 16.4175005030165 41.07552912545391 21.89063942180249 20.616330949727093 100-101 16.001635166295134 42.24573483479072 22.306925823491465 19.445704175422684 102-103 15.636597065642984 43.84928365664062 21.736214462279396 18.77790481543699 104-105 14.678066900250087 45.43442363770509 21.40226705803068 18.485242404014144 106-107 14.26267413562938 46.56040214871071 20.91288268320569 18.264041032454216 108-109 13.956399823708635 47.404492874890614 20.18791637657369 18.451190924827063 110-111 14.046232067348843 48.39739904699856 19.92232910486848 17.634039780784118 112-113 14.156180134142446 48.97540721814117 19.683807090386455 17.184605557329927 114-115 13.91569843571351 49.501778904807836 19.203229494688838 17.37929316478982 116-117 13.713041089940534 49.57843368953557 19.315721229696152 17.39280399082774 118-119 13.62686919917471 49.30054742543963 19.716897368908533 17.35568600647712 120-121 13.529300923308837 48.8670224487645 19.66829866533807 17.935377962588586 122-123 14.191774347051272 48.62927075415658 19.261747967220025 17.91720693157212 124-125 13.994502684735362 49.239644592175914 18.897021222193814 17.86883150089491 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4323.0 1 4038.0 2 4090.5 3 3595.5 4 2193.0 5 1308.0 6 899.5 7 800.0 8 820.0 9 798.5 10 620.5 11 469.5 12 439.5 13 436.5 14 451.0 15 463.0 16 472.0 17 496.5 18 549.0 19 620.5 20 711.0 21 800.5 22 899.0 23 1035.5 24 1210.0 25 1394.5 26 1588.0 27 1794.5 28 1958.5 29 2196.0 30 2521.0 31 2809.5 32 3171.0 33 3558.0 34 3923.5 35 4178.0 36 4402.0 37 4817.5 38 4948.0 39 4770.5 40 4696.0 41 4638.5 42 4354.5 43 4257.0 44 4281.5 45 4208.5 46 4109.0 47 3826.0 48 3642.5 49 3397.0 50 3081.5 51 2740.0 52 2443.5 53 2320.5 54 2303.0 55 2362.5 56 2461.5 57 2374.5 58 2226.0 59 2362.5 60 2573.0 61 2766.5 62 2354.5 63 1234.0 64 547.5 65 451.0 66 413.5 67 329.5 68 223.5 69 151.5 70 131.0 71 134.5 72 135.0 73 176.5 74 149.5 75 76.5 76 48.5 77 34.0 78 31.5 79 20.5 80 10.5 81 5.5 82 4.0 83 5.0 84 4.5 85 3.0 86 2.5 87 2.5 88 2.5 89 3.0 90 2.0 91 1.0 92 3.5 93 3.5 94 3.0 95 2.5 96 2.0 97 2.0 98 1.0 99 2.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017245894518999227 2 0.01213599984670316 3 0.01213599984670316 4 0.029381894365702387 5 0.026826947029554352 6 0.02554947336148034 7 0.008942315676518117 8 0.0 9 0.010858526178629143 10-11 0.0 12-13 0.002874315753166538 14-15 0.017245894518999227 16-17 9.581052510555125E-4 18-19 0.0070261051744070924 20-21 0.002874315753166538 22-23 0.0 24-25 0.0070261051744070924 26-27 0.0 28-29 0.004471157838259058 30-31 3.193684170185042E-4 32-33 6.387368340370084E-4 34-35 0.0 36-37 0.0054292630893145715 38-39 3.193684170185042E-4 40-41 0.011177894595647647 42-43 0.0070261051744070924 44-45 9.581052510555125E-4 46-47 0.011497263012666152 48-49 0.0025549473361480336 50-51 0.008942315676518117 52-53 0.004471157838259058 54-55 0.002235578919129529 56-57 0.007984210425462605 58-59 0.016926526101980723 60-61 0.0015968420850925211 62-63 0.006387368340370085 64-65 0.0015968420850925211 66-67 0.0012774736680740168 68-69 0.0 70-71 0.004471157838259058 72-73 0.0 74-75 0.0054292630893145715 76-77 0.015010315599869699 78-79 0.0015968420850925211 80-81 0.0031936841701850423 82-83 9.581052510555125E-4 84-85 0.0 86-87 0.0012774736680740168 88-89 0.004151789421240555 90-91 0.004790526255277563 92-93 0.013094105097758671 94-95 0.0 96-97 6.387368340370084E-4 98-99 0.002235578919129529 100-101 0.0 102-103 0.0 104-105 0.007984210425462605 106-107 0.0 108-109 0.0 110-111 6.387368340370084E-4 112-113 0.005748631506333076 114-115 0.0012774736680740168 116-117 0.0 118-119 0.005109894672296067 120-121 0.0015968420850925211 122-123 0.0 124-125 0.076009683250404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 156559.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.169124738916324 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9173483841922 48.23229581180258 2 8.58579758205317 9.988566610670738 3 3.3117745884988308 5.779290874366852 4 1.649298883264338 3.8375308988943466 5 0.9048084419506089 2.6315957562324748 6 0.5709956187066949 1.9928589221954662 7 0.40738341257727656 1.6587995579941106 8 0.28549780935334745 1.3285726147969774 9 0.24267313795034534 1.27044756289961 >10 1.0288901821695637 10.350091658735684 >50 0.052707287880617994 2.14998818336857 >100 0.03623626041792487 3.9243991083233793 >500 0.0032942054925386246 1.2525629315465736 >1k 0.0021961369950257497 1.998607553701799 >5k 0.0010980684975128749 3.6043919544708385 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5643 3.6043919544708385 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1612 1.0296437764676576 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1517 0.9689637772341417 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 798 0.5097119935615327 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 607 0.3877132582604641 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 556 0.3551376797245767 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.2721018912997656 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 395 0.25230104944461834 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 331 0.21142189206624978 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 318 0.20311831322376867 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 298 0.1903435765430285 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 284 0.18140126086651037 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 246 0.15712926117310408 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 240 0.153296840168882 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 232 0.14818694549658595 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 213 0.1360509456498828 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 208 0.13285726147969776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 204 0.1303023141435497 No Hit GAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.11369515645858749 No Hit CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT 177 0.11305641962455049 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 172 0.10986273545436545 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 168 0.10730778811821741 No Hit GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA 159 0.10155915661188433 No Hit GAACAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.10028168294381032 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.387368340370084E-4 0.0 10-11 0.0 0.0 0.0 6.387368340370084E-4 0.0 12-13 0.0 0.0 0.0 6.387368340370084E-4 0.0 14-15 0.0 0.0 0.0 6.387368340370084E-4 0.0 16-17 0.0 0.0 0.0 6.387368340370084E-4 0.0 18-19 0.0 0.0 0.0 0.0012774736680740168 0.0 20-21 0.0 0.0 0.0 0.005109894672296067 0.0 22-23 0.0 0.0 0.0 0.01213599984670316 0.0 24-25 0.0 0.0 0.0 0.026826947029554352 0.0 26-27 0.0 0.0 0.0 0.05046020988892366 0.0 28-29 0.0 0.0 0.0 0.09229747251834772 0.0 30-31 0.0 0.0 0.0 0.19225978704513952 0.0 32-33 0.0 0.0 0.0 0.3104261013419861 0.0 34-35 0.0 0.0 0.0 0.4346604155621842 0.0 36-37 0.0 0.0 0.0 0.6004126239947879 0.0 38-39 0.0 0.0 0.0 0.8718757784605164 0.0 40-41 0.0 0.0 0.0 1.2250972476829822 0.0 42-43 0.0 0.0 0.0 1.5307328227696906 0.0 44-45 0.0 0.0 0.0 1.8076252403247337 0.0 46-47 0.0 0.0 0.0 2.114857657496535 0.0 48-49 0.0 0.0 0.0 2.486283126489055 0.0 50-51 0.0 0.0 0.0 2.926372805140554 0.0 52-53 0.0 0.0 0.0 3.3169603791541844 0.0 54-55 0.0 0.0 0.0 3.658684585363984 0.0 56-57 0.0 0.0 0.0 4.025958264935264 0.0 58-59 0.0 0.0 0.0 4.468283522505892 0.0 60-61 0.0 0.0 0.0 5.012167936688405 0.0 62-63 0.0 0.0 0.0 5.5378483511008625 0.0 64-65 0.0 0.0 0.0 5.987838450679936 0.0 66-67 0.0 0.0 0.0 6.4720009708799875 0.0 68-69 0.0 0.0 0.0 7.013011069309334 0.0 70-71 0.0 0.0 0.0 7.639611903499639 0.0 72-73 0.0 0.0 0.0 8.298788316225831 0.0 74-75 0.0 0.0 0.0 8.883871256203731 0.0 76-77 0.0 0.0 0.0 9.437975459730836 0.0 78-79 0.0 0.0 0.0 10.071602399095548 0.0 80-81 0.0 0.0 0.0 10.787945758468053 0.0 82-83 0.0 0.0 0.0 11.572953327499537 0.0 84-85 0.0 0.0 0.0 12.3023907919698 0.0 86-87 0.0 0.0 0.0 13.02639899335075 0.0 88-89 0.0 0.0 0.0 13.752962142067847 0.0 90-91 0.0 0.0 0.0 14.517530132410146 0.0 92-93 0.0 0.0 0.0 15.414316647398106 0.0 94-95 0.0 0.0 0.0 16.25233937365466 0.0 96-97 0.0 0.0 0.0 17.01882357449907 0.0 98-99 0.0 0.0 0.0 17.784349670092425 0.0 100-101 0.0 0.0 0.0 18.66101597480822 0.0 102-103 0.0 0.0 0.0 19.591016805166106 0.0 104-105 0.0 0.0 0.0 20.482693425481767 0.0 106-107 0.0 0.0 0.0 21.326784151661673 0.0 108-109 0.0 0.0 0.0 22.175346035679837 0.0 110-111 0.0 0.0 0.0 23.087462234684686 0.0 112-113 0.0 0.0 0.0 24.017143696625553 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 20 8.4676256E-4 89.25048 1 GGTATCA 550 0.0 86.54591 1 CGTCACT 40 1.9737254E-6 74.3754 1 GAACAAA 680 0.0 63.00034 1 GTACAAG 150 0.0 59.50032 1 CGCGTAA 15 0.0042335866 59.50032 98-99 GTATCAA 1165 0.0 59.24495 1 GTACATA 55 1.30541775E-5 54.0912 1 GAGAGCG 45 3.167369E-4 52.87228 9 CCTTTAG 35 0.0077835824 51.000275 1 CATTCCG 35 0.0077934223 50.98399 9 GGTGGCG 35 0.0077934223 50.98399 9 AACGCAA 35 0.0077934223 50.98399 6 GTGGTAT 130 0.0 50.34642 1 GTCACTA 95 2.6084308E-9 50.105534 1 TACATGA 135 0.0 48.481743 2 CGCAGAA 75 1.4625675E-6 47.600258 2 GTAACAA 90 9.65174E-8 46.278027 1 TACAAGG 65 3.506069E-5 45.769474 2 AGAACAA 365 0.0 45.64408 2 >>END_MODULE