##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940129_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156559 Sequences flagged as poor quality 0 Sequence length 125 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50732311780224 33.0 31.0 34.0 31.0 34.0 2 32.44942162379678 33.0 31.0 34.0 31.0 34.0 3 32.46661641936905 34.0 31.0 34.0 30.0 34.0 4 36.067227051782396 37.0 35.0 37.0 35.0 37.0 5 36.13982588033904 37.0 35.0 37.0 35.0 37.0 6 36.266033891376416 37.0 37.0 37.0 35.0 37.0 7 36.199937403790265 37.0 37.0 37.0 35.0 37.0 8 36.23561085597123 37.0 37.0 37.0 35.0 37.0 9 37.94022700707082 39.0 39.0 39.0 35.0 39.0 10-11 37.95369157953232 39.0 39.0 39.0 35.0 39.0 12-13 37.942849021774535 39.0 39.0 39.0 35.0 39.0 14-15 38.93161364086383 40.5 38.0 41.0 35.5 41.0 16-17 39.05300238248839 40.0 38.5 41.0 36.0 41.0 18-19 39.44442350807044 41.0 39.0 41.0 36.0 41.0 20-21 39.486634431747774 41.0 39.0 41.0 36.0 41.0 22-23 39.31620028232168 41.0 39.0 41.0 35.0 41.0 24-25 39.19434845649244 41.0 39.0 41.0 35.0 41.0 26-27 38.88610683512286 41.0 38.0 41.0 35.0 41.0 28-29 38.57980058636041 40.5 37.5 41.0 35.0 41.0 30-31 37.693246635453725 39.0 36.0 41.0 34.5 41.0 32-33 36.65782229063804 37.5 35.0 41.0 33.5 41.0 34-35 36.07801531690928 37.0 35.0 41.0 33.0 41.0 36-37 35.620730842685504 36.0 35.0 41.0 32.5 41.0 38-39 35.18212622717314 36.0 35.0 41.0 30.5 41.0 40-41 34.74015227486123 36.0 35.0 41.0 28.0 41.0 42-43 34.08935928308178 35.5 35.0 41.0 21.5 41.0 44-45 33.164350181081886 35.5 34.5 41.0 7.5 41.0 46-47 31.372163210035833 35.0 32.0 41.0 2.0 41.0 48-49 29.833682509469273 35.0 24.5 41.0 2.0 41.0 50-51 29.38232551306536 35.0 23.5 41.0 2.0 41.0 52-53 29.358251521790507 35.0 24.0 41.0 2.0 41.0 54-55 29.210013477347196 35.0 24.0 40.5 2.0 41.0 56-57 29.044963879432036 35.0 23.5 40.0 2.0 41.0 58-59 28.81394234761336 35.0 23.0 40.0 2.0 41.0 60-61 28.56060335081343 35.0 22.0 40.0 2.0 41.0 62-63 28.32428349695642 35.0 21.0 39.5 2.0 41.0 64-65 27.99916644843158 35.0 19.0 39.0 2.0 41.0 66-67 27.614480802764454 35.0 12.0 39.0 2.0 41.0 68-69 27.22434992558716 35.0 6.0 38.0 2.0 41.0 70-71 26.858944551255437 35.0 2.0 37.0 2.0 41.0 72-73 26.593606883028123 35.0 2.0 37.0 2.0 40.0 74-75 26.34037327780581 35.0 2.0 36.5 2.0 39.0 76-77 26.05040272357386 35.0 2.0 36.0 2.0 39.0 78-79 25.75163037576888 35.0 2.0 35.5 2.0 39.0 80-81 25.45047873325711 35.0 2.0 35.0 2.0 37.0 82-83 25.14201994136396 35.0 2.0 35.0 2.0 37.0 84-85 24.890178782439847 35.0 2.0 35.0 2.0 36.5 86-87 24.649097145485086 35.0 2.0 35.0 2.0 36.0 88-89 24.43442088924942 35.0 2.0 35.0 2.0 36.0 90-91 24.23771549383938 35.0 2.0 35.0 2.0 36.0 92-93 24.035651096391774 35.0 2.0 35.0 2.0 35.0 94-95 23.843206075664767 34.5 2.0 35.0 2.0 35.0 96-97 23.673394694651854 34.0 2.0 35.0 2.0 35.0 98-99 23.506828096755854 34.0 2.0 35.0 2.0 35.0 100-101 23.36450475539573 34.0 2.0 35.0 2.0 35.0 102-103 22.65169041703128 32.5 2.0 35.0 2.0 35.0 104-105 22.989128699084688 34.0 2.0 35.0 2.0 35.0 106-107 22.90769294642914 34.0 2.0 35.0 2.0 35.0 108-109 22.763319259831757 34.0 2.0 35.0 2.0 35.0 110-111 22.58443462209135 34.0 2.0 35.0 2.0 35.0 112-113 22.36877790481544 34.0 2.0 35.0 2.0 35.0 114-115 22.120398699531805 34.0 2.0 35.0 2.0 35.0 116-117 21.878218435222504 34.0 2.0 35.0 2.0 35.0 118-119 21.649943471790188 33.0 2.0 35.0 2.0 35.0 120-121 21.398150218128627 33.0 2.0 35.0 2.0 35.0 122-123 21.106250678657887 33.0 2.0 35.0 2.0 35.0 124-125 19.88886298456173 29.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41960519022637044 1101 2 0.21678876643598244 1101 3 0.20976316225475955 1101 4 0.21624170586334657 1101 5 0.056787385889585096 1101 6 0.09868955752728681 1101 7 0.18942992899215483 1101 8 0.13839303874252096 1101 9 0.21318733840752202 1101 10-11 0.15065443505644538 1101 12-13 0.36326122513408166 1101 14-15 0.4863968693013021 1101 16-17 0.5175091290647131 1101 18-19 0.38651308431514053 1101 20-21 0.46542561072258337 1101 22-23 0.37717292922854995 1101 24-25 0.46270676779245434 1101 26-27 0.3449620684440191 1101 28-29 0.47224832831668806 1101 30-31 0.6189548982178863 1101 32-33 0.8729824774670689 1101 34-35 0.6930907465061935 1101 36-37 0.48000359532074555 1101 38-39 0.42460949487821864 1101 40-41 1.0544272847770628 1101 42-43 1.0849352245784303 1101 44-45 0.7155097204800285 1101 46-47 0.5677856159320811 1101 48-49 1.1824097095298463 1101 50-51 1.130413899215732 1101 52-53 0.8002315971836786 1101 54-55 0.6749259248502639 1101 56-57 1.0573020329092238 1101 58-59 0.9024189316078548 1101 60-61 0.9082876541870348 1101 62-63 0.9138886747250972 1101 64-65 0.8214593257319827 1101 66-67 0.6900898829691791 1101 68-69 0.9455250684656633 1101 70-71 0.8933923806813979 1101 72-73 0.7267536709765565 1101 74-75 0.1889514286444367 1101 76-77 -0.021353229761274406 1101 78-79 0.29401402775256713 1101 80-81 -0.056111373629043726 1101 82-83 0.023127893273770184 1101 84-85 -0.014022048458823377 1101 86-87 0.02134818844078268 1101 88-89 0.09294613568132704 1101 90-91 -0.010998472375277402 1101 92-93 -0.06549994600552012 1101 94-95 -0.012122191206472621 1101 96-97 0.17006415245241868 1101 98-99 0.32566812836950376 1101 100-101 0.3469969176794834 1101 102-103 0.22874062005537965 1101 104-105 0.4402648778430489 1101 106-107 0.5825490702474632 1101 108-109 0.21435116606895122 1101 110-111 0.14130349059749392 1101 112-113 -0.04925915714739304 1101 114-115 -0.022887812076447744 1101 116-117 -0.15203804685986455 1101 118-119 -1.705780135914381E-4 1101 120-121 -0.17377542435870552 1101 122-123 -0.2388385222884999 1101 124-125 -0.8416023334339577 1102 1 -0.06980574801615802 1102 2 0.22714021997841627 1102 3 0.25240867145949863 1102 4 0.03134119138990599 1102 5 -0.03284256514246664 1102 6 0.010547240537263747 1102 7 0.06969605019300928 1102 8 -0.09126046649519282 1102 9 0.2144270334425471 1102 10-11 0.014411950707597043 1102 12-13 0.08045579526982749 1102 14-15 0.05379495603862949 1102 16-17 0.25063378040048434 1102 18-19 -0.19062693923905982 1102 20-21 -0.11524754368142709 1102 22-23 -0.10307459654677587 1102 24-25 -0.19829862488094818 1102 26-27 -0.2696787299195904 1102 28-29 -0.1807816929647501 1102 30-31 0.13052538521142054 1102 32-33 0.18142893297235219 1102 34-35 0.24588109341418374 1102 36-37 0.27821223599514155 1102 38-39 0.1681682460920868 1102 40-41 0.4661816598674591 1102 42-43 0.6300911162290888 1102 44-45 0.7235677382076702 1102 46-47 0.2113939549473507 1102 48-49 0.6920793308030113 1102 50-51 0.8912457345842313 1102 52-53 0.5761050656437696 1102 54-55 0.6335407722644639 1102 56-57 0.2120056424879344 1102 58-59 0.7704843875057037 1102 60-61 0.9291248615673524 1102 62-63 0.7867476180916952 1102 64-65 0.5281268188620096 1102 66-67 0.5815029286932685 1102 68-69 0.7743438457131262 1102 70-71 0.19066091928756634 1102 72-73 0.10496396457766366 1102 74-75 0.48226327393049573 1102 76-77 0.44779902545061034 1102 78-79 0.47092884004207747 1102 80-81 0.7656204803348814 1102 82-83 0.7811704148134702 1102 84-85 0.7960050182161069 1102 86-87 1.0006246197720046 1102 88-89 0.5294530883043116 1102 90-91 0.5182789920351496 1102 92-93 0.5439651255868654 1102 94-95 0.7374077577594136 1102 96-97 0.8582188663927894 1102 98-99 1.014029458149043 1102 100-101 0.840261241322537 1102 102-103 0.9540861850463926 1102 104-105 0.6657963970893164 1102 106-107 0.6352051168599964 1102 108-109 0.7214484201444478 1102 110-111 0.7535165528508472 1102 112-113 0.8423811659997824 1102 114-115 1.085719803783384 1102 116-117 0.9436974022196587 1102 118-119 1.1954842908886576 1102 120-121 1.438148375719809 1102 122-123 1.2490227971602472 1102 124-125 1.0771976417721412 1103 1 0.19358916631504286 1103 2 -0.03596145150581265 1103 3 0.053841372226116846 1103 4 -0.050913427184930526 1103 5 0.21071656518303428 1103 6 0.2594475798611313 1103 7 0.3782713088750924 1103 8 0.2900390182352339 1103 9 0.22422972289837162 1103 10-11 0.2137018840995779 1103 12-13 0.06440074939826701 1103 14-15 0.25309536238943053 1103 16-17 0.15320384449499613 1103 18-19 0.3149970396474515 1103 20-21 0.452805742733041 1103 22-23 0.6408825062138988 1103 24-25 0.4918798343373467 1103 26-27 0.30409279201078476 1103 28-29 0.33683382377103754 1103 30-31 0.18455537976549152 1103 32-33 0.13938000330873734 1103 34-35 0.03869454907167835 1103 36-37 -0.05457650347953802 1103 38-39 0.2205225672349016 1103 40-41 0.38246082705776985 1103 42-43 0.3215577493822863 1103 44-45 0.7161843022393342 1103 46-47 0.003222952475734786 1103 48-49 -0.8488908841021008 1103 50-51 -0.3503968442771459 1103 52-53 -0.25883063038907395 1103 54-55 -0.3507393165462247 1103 56-57 -0.054761564583404976 1103 58-59 -0.07735399379378549 1103 60-61 -0.05687279248987309 1103 62-63 0.014733458651370768 1103 64-65 -0.12743851145511442 1103 66-67 -0.06413403666218542 1103 68-69 0.16922675803351694 1103 70-71 0.22551492624161185 1103 72-73 -0.19644779027131776 1103 74-75 -0.30713599715156903 1103 76-77 -0.22150682683877676 1103 78-79 -0.013937734462551532 1103 80-81 0.03535153475237962 1103 82-83 0.17572866541042842 1103 84-85 0.0747113983426182 1103 86-87 0.29745969161650265 1103 88-89 0.30690271337238784 1103 90-91 0.367981560004651 1103 92-93 0.07192792563714079 1103 94-95 0.1322383052856999 1103 96-97 -0.09801463869706595 1103 98-99 -0.18521858340765718 1103 100-101 0.029507607205825792 1103 102-103 0.0350526296863336 1103 104-105 0.20098553872561098 1103 106-107 0.16304224140442258 1103 108-109 0.0862844136093237 1103 110-111 -0.01070572522715807 1103 112-113 -0.06460848671690655 1103 114-115 0.11668834301509179 1103 116-117 -0.09837247336158939 1103 118-119 -0.1858466127182261 1103 120-121 0.026990485392634156 1103 122-123 -0.1023135523852261 1103 124-125 -0.016713379969719 1104 1 0.1989918166718212 1104 2 0.2855268257622825 1104 3 0.23341323766954503 1104 4 -0.010485292628359844 1104 5 -0.030955198323589173 1104 6 0.07471771237896974 1104 7 -0.17748812027883076 1104 8 0.11434075116082454 1104 9 0.028551843183791448 1104 10-11 0.22253980458887668 1104 12-13 0.2737891286031555 1104 14-15 0.2885285937859692 1104 16-17 0.26147091890885577 1104 18-19 0.21001233016733067 1104 20-21 0.20913601796240044 1104 22-23 0.4298325571822943 1104 24-25 0.2094639321966767 1104 26-27 -0.22298162185261106 1104 28-29 -0.22540878581101964 1104 30-31 -0.3302356497961867 1104 32-33 -0.15412662258320609 1104 34-35 0.2925477600808506 1104 36-37 0.6817129970301465 1104 38-39 0.4438151258485519 1104 40-41 0.5706565457122039 1104 42-43 0.33480953327627105 1104 44-45 0.35760122374799863 1104 46-47 0.05489471598236051 1104 48-49 0.5324750690069706 1104 50-51 0.23155014858727796 1104 52-53 0.4876423563591423 1104 54-55 0.8443779107728382 1104 56-57 0.845034561818224 1104 58-59 0.7192515107933737 1104 60-61 0.6121690015977954 1104 62-63 0.945834376082562 1104 64-65 0.9547021613277629 1104 66-67 0.5281391539596321 1104 68-69 0.5343134043128224 1104 70-71 0.05248740330583246 1104 72-73 0.3527265216552884 1104 74-75 0.14685992537646086 1104 76-77 0.21203038009292285 1104 78-79 0.016286526495655096 1104 80-81 -0.04019382712326092 1104 82-83 -0.03919488199018062 1104 84-85 -0.00865251603046957 1104 86-87 -0.01830993121733826 1104 88-89 0.10321260124191056 1104 90-91 0.16089695246132862 1104 92-93 0.17696360351685314 1104 94-95 0.08879284147326771 1104 96-97 0.20156742042627585 1104 98-99 0.01010251750977531 1104 100-101 0.19788681209879044 1104 102-103 0.4834012535395438 1104 104-105 -0.009880924067459773 1104 106-107 -0.13106581160271347 1104 108-109 0.15609073369087056 1104 110-111 0.3917509516331883 1104 112-113 0.5394587915705564 1104 114-115 0.5745782512719693 1104 116-117 0.5771525011542131 1104 118-119 0.3789546105233619 1104 120-121 0.45279068926623367 1104 122-123 0.442569220295713 1104 124-125 0.22980038149816906 1105 1 0.07960965424032906 1105 2 -0.1835115399576992 1105 3 0.14994537518823137 1105 4 -0.31966317005735334 1105 5 -0.20945046489060815 1105 6 0.08659613788967846 1105 7 -0.06069688958626074 1105 8 -0.008270110822238053 1105 9 0.037081987678064365 1105 10-11 0.48400467753356935 1105 12-13 0.34278763383485256 1105 14-15 0.4000283446579118 1105 16-17 0.26614456116097074 1105 18-19 0.33895104466658665 1105 20-21 0.1522750314153214 1105 22-23 0.2725729657523033 1105 24-25 0.4078196870546691 1105 26-27 0.42167209015535434 1105 28-29 0.2929370039249122 1105 30-31 0.32732289529599257 1105 32-33 0.09697452340583368 1105 34-35 0.5268975358656078 1105 36-37 1.0391619257795242 1105 38-39 1.2392311696950955 1105 40-41 0.7554398043071231 1105 42-43 0.585432353405821 1105 44-45 1.1627531918596148 1105 46-47 1.193569354746689 1105 48-49 1.6886086016427448 1105 50-51 1.3966821864547398 1105 52-53 1.2535217783820656 1105 54-55 1.7312638101251032 1105 56-57 1.604924945475421 1105 58-59 1.6575474749189354 1105 60-61 1.8340125491812493 1105 62-63 2.0566863940197813 1105 64-65 2.261996630685008 1105 66-67 2.106146129545081 1105 68-69 2.2100483320656856 1105 70-71 2.2382273136197313 1105 72-73 2.433802754839146 1105 74-75 2.239465804798485 1105 76-77 2.1367438828333327 1105 78-79 1.7996447726341707 1105 80-81 1.578600393105937 1105 82-83 1.357157883331194 1105 84-85 1.6994491282146704 1105 86-87 1.5730901684338825 1105 88-89 1.8548518638228764 1105 90-91 1.803298546880857 1105 92-93 1.9211688850315518 1105 94-95 1.7966353925445162 1105 96-97 2.0032216306903514 1105 98-99 1.9680397372407121 1105 100-101 1.9186341962542457 1105 102-103 2.0702173970373003 1105 104-105 1.7950119508702578 1105 106-107 1.8806133114665435 1105 108-109 1.7342870465957034 1105 110-111 1.9593543397746949 1105 112-113 2.245497655546643 1105 114-115 2.2291970853526877 1105 116-117 2.038697095075488 1105 118-119 2.0341414565622244 1105 120-121 2.106442906505894 1105 122-123 2.1609748007640697 1105 124-125 2.339038049659603 1106 1 -0.07211929443928966 1106 2 -0.2211398409043852 1106 3 0.03785768211398732 1106 4 -0.023818625961688156 1106 5 0.07126702389863482 1106 6 0.008051045712299754 1106 7 0.04473410194339067 1106 8 -0.2012148043947306 1106 9 0.09521850985046143 1106 10-11 -0.15968241763334845 1106 12-13 -0.03732198250794738 1106 14-15 -0.13591585065847767 1106 16-17 -0.08964019220225339 1106 18-19 0.1789012190562218 1106 20-21 -0.0775306487042613 1106 22-23 -0.6123896650399274 1106 24-25 -0.40386940113666014 1106 26-27 -0.3007593996303939 1106 28-29 -0.4587421191443539 1106 30-31 -0.6924578720184087 1106 32-33 -0.6519044003609835 1106 34-35 -0.49411890658581115 1106 36-37 -0.45828700296985403 1106 38-39 -0.7184070963736673 1106 40-41 -0.5160101209544621 1106 42-43 0.20369842216516076 1106 44-45 0.17760122374800602 1106 46-47 -0.49843861735097406 1106 48-49 -0.49641381988191924 1106 50-51 -0.4995609625238302 1106 52-53 -0.31902431030752254 1106 54-55 -0.1489554225604941 1106 56-57 0.17836789515155616 1106 58-59 0.5325848441267063 1106 60-61 0.7810578904866823 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0.27748135904869997 2216 56-57 0.035839159519373 2216 58-59 -0.9045799068311453 2216 60-61 -0.4969497723485681 2216 62-63 -0.965889761848473 2216 64-65 -0.8881330877143832 2216 66-67 -0.8190639111744709 2216 68-69 -1.0189433006680204 2216 70-71 -1.41134401431869 2216 72-73 -1.3290359304519903 2216 74-75 -1.1355921819032275 2216 76-77 -1.4404600413630142 2216 78-79 -1.0906100252284823 2216 80-81 -1.416975436318662 2216 82-83 -1.4164362613005288 2216 84-85 -1.5194571137316188 2216 86-87 -1.8013750653169573 2216 88-89 -1.935408088413265 2216 90-91 -1.8720532391095546 2216 92-93 -1.735833331349049 2216 94-95 -1.9199427907106426 2216 96-97 -2.0296203190373276 2216 98-99 -2.138020087854212 2216 100-101 -2.1606572492038936 2216 102-103 -2.2693956813263547 2216 104-105 -2.2391529547188007 2216 106-107 -2.1286903326755073 2216 108-109 -1.9641391513665987 2216 110-111 -2.031084297408956 2216 112-113 -1.8706561509581796 2216 114-115 -2.067720599302742 2216 116-117 -2.0402421348611135 2216 118-119 -1.8314668454153384 2216 120-121 -1.4773242532625055 2216 122-123 -1.3493081743403046 2216 124-125 -1.475103833061219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 10.0 5 40.0 6 93.0 7 222.0 8 499.0 9 876.0 10 1909.0 11 2572.0 12 3072.0 13 4726.0 14 8947.0 15 8946.0 16 2564.0 17 1898.0 18 1767.0 19 1755.0 20 1883.0 21 2054.0 22 2022.0 23 1424.0 24 1551.0 25 1657.0 26 1778.0 27 1916.0 28 1960.0 29 2180.0 30 2484.0 31 2909.0 32 3547.0 33 4250.0 34 6189.0 35 15464.0 36 15059.0 37 28435.0 38 19726.0 39 172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.59102612429003 17.580388574057153 16.92935772196702 12.899227579685792 2 16.861597817963474 23.753281678175163 42.56568147121385 16.819439032647505 3 15.648813516016736 36.48047012232123 35.73887771070869 12.13183865095334 4 8.779118827831335 21.38108267062163 38.43365637865344 31.4061421228936 5 7.163662378476436 40.15611226797143 31.850990712469816 20.829234641082312 6 19.393707075547038 43.443539370707555 16.63695895224405 20.525794601501357 7 17.02744652175857 44.87637248577214 20.93523847239699 17.160942520072307 8 26.282104510120785 30.176482987244423 19.3613909133298 24.18002158930499 9 30.325308669575048 11.826212482195212 20.92374120938432 36.924737638845414 10-11 22.477468558179346 29.73128341392063 23.88779948773306 23.903448540166966 12-13 30.300717301464626 30.13432635619798 27.26799481345691 12.296961528880486 14-15 24.47607611188114 25.019321789229622 36.57470985379314 13.929892245096099 16-17 13.046199835205897 29.51187731142892 35.5677412349338 21.87418161843139 18-19 13.015221098755102 18.32727597902388 53.09308311882421 15.564419803396804 20-21 16.10095874398789 16.076367375877464 55.52443487758608 12.29823900254856 22-23 14.75545960308893 17.91177766848281 55.53529340376472 11.797469324663545 24-25 13.282297934311885 17.878038809897927 56.84570574483578 11.993957510954406 26-27 13.042048045784657 17.9012385107212 56.72877317816286 12.32794026533128 28-29 13.368165893260347 16.941590523670065 56.82604266136939 12.864200921700194 30-31 13.335036729479398 17.677738741616096 56.443947620568515 12.543276908335995 32-33 12.911426363224088 17.515122094545827 56.14177402768286 13.43167751454723 34-35 13.1135865711968 17.470091147746217 56.46210054995241 12.954221731104568 36-37 13.588298229085161 17.5967296360762 54.23917729907542 14.575794835763217 38-39 13.734438773880772 17.449012832222994 54.36768247114506 14.448865922751168 40-41 13.809809720297139 17.54354588366047 53.647826059185356 14.998818336857031 42-43 14.036880664797296 17.889102510874494 52.57985807267548 15.494158751652732 44-45 14.286854930917963 18.49501446794376 50.91215115647736 16.305979444660913 46-47 15.594370083643923 18.978451868814133 47.20053143753214 18.226646610009805 48-49 16.071896218039207 20.357501006010516 43.60113439661725 19.96946837933303 50-51 16.42548489193928 21.256998316890815 42.21314485917135 20.104371931998557 52-53 15.978321271852783 22.222931929815598 41.50575821255884 20.292988585772775 54-55 16.045211092622697 22.022362819337673 41.15148398800418 20.780942100035453 56-57 16.86749681428987 22.164238926663195 39.73722282724988 21.23104143179706 58-59 16.28097114788863 21.777371819848362 41.079287416084874 20.862369616178135 60-61 17.4352799902912 22.303093402487242 40.20944180788074 20.052184799340825 62-63 18.532701385473292 22.26242869826832 40.80439212279548 18.400477793462915 64-65 18.073201111430485 22.566829548720897 41.32126089872569 18.038708441122928 66-67 18.089985245179133 22.70645571318161 40.6144009606602 18.589158080979058 68-69 18.636543645166647 22.516575326715977 40.78712042821191 18.059760599905466 70-71 18.094541552356834 22.82129979464152 40.30519528218427 18.77896337081738 72-73 17.82211420231164 22.69802082967382 39.89926894935023 19.580596018664306 74-75 16.915667575802093 23.238842864351458 40.00951717882715 19.835972381019296 76-77 16.098558037781647 23.367452852785718 40.42125097807514 20.11273813135749 78-79 15.921256020133114 22.743008980697248 40.74560226880772 20.59013273036191 80-81 15.791810116313979 22.8287738168997 40.34772833244975 21.031687734336575 82-83 16.025102598086967 22.114877920252944 39.62761285789566 22.23240662376443 84-85 16.21401516361244 21.689586673394697 39.27433108285056 22.82206708014231 86-87 15.794028973657989 22.10486982293876 38.62739211528143 23.473709088121822 88-89 16.00097091719737 21.77697009958289 39.238149380720905 22.983909602498834 90-91 15.981910164034954 22.176976340129798 38.95766262966938 22.883450866165873 92-93 15.918353007307475 22.41236138790945 38.68043844856661 22.988847156216465 94-95 16.032116611148194 22.08623833872549 38.74912570382838 23.132519346297933 96-97 16.294912331129634 22.35667976110632 38.56951231196704 22.778895595797007 98-99 16.190156813899268 22.30462137906806 38.27440835489126 23.23081345214142 100-101 16.66480359498312 22.255615564207307 38.55121158213112 22.528369258678456 102-103 17.045338409259305 22.568921025064515 37.80628018089374 22.579460384782443 104-105 16.708876930201363 22.461395972725676 37.855739903869186 22.973987193203772 106-107 16.47430042348252 22.633320345684375 37.58135910423546 23.31102012659764 108-109 16.667198977962315 22.846055573299264 36.936442031299904 23.550303417438517 110-111 16.57798757002242 23.106600152021308 36.82620387462713 23.48920840332914 112-113 16.360912917670053 23.128908420790403 36.54034097078945 23.969837690750097 114-115 16.003551376797244 23.48220159811956 36.27705848913189 24.237188535951304 116-117 15.719952222484816 23.55916938662102 36.654871326464786 24.066007064429385 118-119 15.655420424640385 23.746454942640334 36.59744755870104 24.000677074018242 120-121 15.52422896233676 24.08971726223744 35.999961675667564 24.386092099758237 122-123 16.205331621491283 24.22480063108207 35.16845356149377 24.401414185932875 124-125 15.507898369204028 24.529037370277592 34.66559736209805 25.29746689842033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1197.0 1 871.0 2 989.5 3 1310.0 4 725.5 5 445.0 6 1119.0 7 2858.0 8 4787.0 9 5012.5 10 3083.5 11 1314.5 12 977.0 13 925.0 14 928.0 15 986.0 16 1053.5 17 1082.0 18 1073.5 19 1055.0 20 1089.5 21 1106.5 22 1131.5 23 1233.0 24 1320.5 25 1480.0 26 1667.0 27 1791.5 28 1938.5 29 2160.5 30 2505.5 31 2769.0 32 2968.0 33 3261.0 34 3525.5 35 3728.0 36 3972.0 37 4344.5 38 4531.0 39 4554.5 40 4479.0 41 4315.0 42 4157.5 43 4108.0 44 4257.0 45 4385.5 46 4496.0 47 4471.5 48 4267.5 49 3996.5 50 3570.5 51 2942.5 52 2426.5 53 2092.5 54 1925.5 55 2033.0 56 2100.0 57 1856.5 58 1662.0 59 1703.5 60 1838.0 61 2064.5 62 1822.0 63 967.0 64 413.5 65 343.5 66 310.0 67 244.5 68 170.0 69 117.0 70 101.5 71 97.0 72 110.0 73 159.0 74 115.5 75 51.0 76 44.0 77 34.0 78 22.5 79 4.5 80 3.5 81 3.5 82 1.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02427199969340632 2 0.005109894672296067 3 0.0025549473361480336 4 0.0836745252588481 5 0.0031936841701850423 6 0.021717052357258286 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.387368340370084E-4 26-27 0.0 28-29 3.193684170185042E-4 30-31 0.005748631506333076 32-33 0.0 34-35 0.0 36-37 9.581052510555125E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.00383242100422205 46-47 0.0015968420850925211 48-49 0.0 50-51 0.002235578919129529 52-53 0.0 54-55 0.002874315753166538 56-57 3.193684170185042E-4 58-59 0.0012774736680740168 60-61 0.0 62-63 0.00383242100422205 64-65 0.0025549473361480336 66-67 0.0 68-69 6.387368340370084E-4 70-71 0.002235578919129529 72-73 0.002235578919129529 74-75 0.0 76-77 9.581052510555125E-4 78-79 6.387368340370084E-4 80-81 0.0 82-83 9.581052510555125E-4 84-85 0.0 86-87 0.001916210502111025 88-89 0.00383242100422205 90-91 0.004471157838259058 92-93 0.004471157838259058 94-95 0.002874315753166538 96-97 0.0025549473361480336 98-99 0.0025549473361480336 100-101 0.004790526255277563 102-103 0.001916210502111025 104-105 9.581052510555125E-4 106-107 0.0 108-109 0.005748631506333076 110-111 0.0012774736680740168 112-113 0.00383242100422205 114-115 0.0 116-117 0.0 118-119 0.001916210502111025 120-121 3.193684170185042E-4 122-123 0.0015968420850925211 124-125 0.04535031521662759 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 156559.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.407392740117146 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8429820907955 31.818675387553576 2 11.228346213768955 9.298730829910768 3 4.379348111126537 5.440121615493201 4 2.321563546053342 3.84519574090279 5 1.2787881592546315 2.6475641770834 6 0.8514970614095979 2.1154963943305716 7 0.5908032147099203 1.7124534520532193 8 0.4056951578817468 1.3439022988138658 9 0.33010936801024265 1.2302071423552783 >10 1.5641630801980655 11.716988483574882 >50 0.10952226695666929 3.348258484021998 >100 0.07404322273126938 5.844442031438627 >500 0.010797969981643452 2.8519599639752427 >1k 0.009255402841408673 7.218364961452233 >5k 0.003085134280469557 9.56763903704035 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8441 5.391577616106388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6538 4.176061420933961 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4750 3.0339999616757902 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1668 1.06541303917373 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1608 1.0270888291315097 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1213 0.7747877796868913 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1059 0.6764223072451919 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1003 0.6406530445391194 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 798 0.5097119935615327 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 779 0.4975759937148295 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 662 0.42284378413249957 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 630 0.4024042054433153 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 572 0.3653574690691688 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 522 0.3334206273673184 TruSeq Adapter, Index 1 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 502 0.3206458906865782 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 409 0.26124336512113644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 407 0.2599658914530624 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 367 0.23441641809158206 RNA PCR Primer, Index 1 (95% over 23bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 346 0.22100294457680492 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 333 0.21269936573432383 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 302 0.19289852387917655 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 283 0.1807625240324734 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 263 0.16798778735173323 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 253 0.16160041901136313 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 240 0.153296840168882 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.152658103334845 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 237 0.151380629666771 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 230 0.14690947182851194 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 214 0.1366896824839198 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 212 0.13541220881584579 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 210 0.13413473514777177 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 207 0.13221852464566072 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 204 0.1303023141435497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 196 0.12519241947125365 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 195 0.12455368263721664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 192 0.1226374721351056 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 184 0.11752757746280955 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 182 0.11625010379473553 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 178 0.11369515645858749 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 166 0.1060303144501434 No Hit GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 165 0.10539157761610639 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.387368340370084E-4 0.0 10-11 0.0 0.0 0.0 6.387368340370084E-4 0.0 12-13 0.0 0.0 0.0 6.387368340370084E-4 0.0 14-15 0.0 0.0 0.0 6.387368340370084E-4 0.0 16-17 0.0 0.0 0.0 6.387368340370084E-4 0.0 18-19 0.0 0.0 0.0 0.0012774736680740168 0.0 20-21 0.0 0.0 0.0 0.005109894672296067 0.0 22-23 0.0 0.0 0.0 0.01213599984670316 0.0 24-25 0.0 0.0 0.0 0.026188210195517345 0.0 26-27 0.0 0.0 0.0 0.049821473054886656 0.0 28-29 0.0 0.0 0.0 0.09133936726729219 0.0 30-31 0.0 0.0 0.0 0.1909823133770655 0.0 32-33 0.0 0.0 0.0 0.30819052242285655 0.0 34-35 0.0 0.0 0.0 0.4330635734770917 0.0 36-37 0.0 0.0 0.0 0.6016900976628619 0.0 38-39 0.0 0.0 0.0 0.878582515217905 0.0 40-41 0.0 0.0 0.0 1.2359557738616114 0.0 42-43 0.0 0.0 0.0 1.5425494541993752 0.0 44-45 0.0 0.0 0.0 1.8207193454224924 0.0 46-47 0.0 0.0 0.0 2.1305067099304416 0.0 48-49 0.0 0.0 0.0 2.5067227051782393 0.0 50-51 0.0 0.0 0.0 2.956074067923275 0.0 52-53 0.0 0.0 0.0 3.354007115528331 0.0 54-55 0.0 0.0 0.0 3.7043542689976303 0.0 56-57 0.0 0.0 0.0 4.087915737836854 0.0 58-59 0.0 0.0 0.0 4.550680574096667 0.0 60-61 0.0 0.0 0.0 5.109255935462031 0.0 62-63 0.0 0.0 0.0 5.648669191806285 0.0 64-65 0.0 0.0 0.0 6.111434028066096 0.0 66-67 0.0 0.0 0.0 6.6074131796958335 0.0 68-69 0.0 0.0 0.0 7.167585383146291 0.0 70-71 0.0 0.0 0.0 7.811112743438576 0.0 72-73 0.0 0.0 0.0 8.4932836821901 0.0 74-75 0.0 0.0 0.0 9.093696306184889 0.0 76-77 0.0 0.0 0.0 9.66792071998416 0.0 78-79 0.0 0.0 0.0 10.328055237961408 0.0 80-81 0.0 0.0 0.0 11.07282238644856 0.0 82-83 0.0 0.0 0.0 11.88369879725854 0.0 84-85 0.0 0.0 0.0 12.630701524664822 0.0 86-87 0.0 0.0 0.0 13.37834298890514 0.0 88-89 0.0 0.0 0.0 14.129816874149682 0.0 90-91 0.0 0.0 0.0 14.921211811521534 0.0 92-93 0.0 0.0 0.0 15.835563589445513 0.0 94-95 0.0 0.0 0.0 16.711910525744287 0.0 96-97 0.0 0.0 0.0 17.519593252384084 0.0 98-99 0.0 0.0 0.0 18.331108400028107 0.0 100-101 0.0 0.0 0.0 19.24130838853084 0.0 102-103 0.0 0.0 0.0 20.21346584993517 0.0 104-105 0.0 0.0 0.0 21.141231101373926 0.0 106-107 0.0 0.0 0.0 22.043766247868216 0.0 108-109 0.0 0.0 0.0 22.935442868183877 0.0 110-111 0.0 0.0 0.0 23.89418685607343 0.0 112-113 0.0 0.0 0.0 24.874328527903216 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1800 0.0 99.64915 1 CCGTTGC 20 8.4702484E-4 89.24354 9 TATAAGG 25 0.0020411238 71.509026 2 GGATCGT 25 0.0020541104 71.39483 8 GGGATCG 25 0.0020541104 71.39483 7 GTGGTAT 225 0.0 68.860535 1 GTATCAA 3945 0.0 64.95327 1 TGGTATC 255 0.0 60.759296 2 CCTACAT 30 0.0042042397 59.590847 3 AAGATTG 45 3.153622E-4 52.91886 5 TATTCCG 35 0.007766334 51.028896 5 TCCGTTG 35 0.0077859852 50.996304 8 GATTGGC 35 0.0077859852 50.996304 9 TATCAAC 5340 0.0 48.096733 2 ATCAACG 5440 0.0 46.99352 3 TCAACGC 5480 0.0 46.541763 4 CAACGCA 5545 0.0 45.952084 5 AACGCAG 5630 0.0 45.243855 6 CTACAGT 55 8.4421085E-4 43.3388 4 GGGATAC 55 8.508839E-4 43.269592 7 >>END_MODULE