FastQCFastQC Report
Fri 27 May 2016
SRR940128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54022
Sequences flagged as poor quality0
Sequence length125
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26074.82581170634186No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6011.11250971826293No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.6293732183184628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.48313649994446706No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.4072414942060642No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.38132612639295105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.36096405168264784No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1840.3406019769723446No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1490.27581355743956165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.2517492873273851No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1260.23323831031801856No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.18881196549553886No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG880.16289659768242568No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.1536411091777424No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG830.1536411091777424No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.15179001147680574No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.14623671837399577No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT780.14438562067305913No Hit
GAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.13698122986931252No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA710.13142793676650255No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.13142793676650255No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT690.12772574136462922No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT620.11476805745807264No Hit
GTGTAGCGCTGGCTGTCCTGGAATTCTTTATAGATCAGTTGGCCTTGAAC610.11291695975713598No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA600.11106586205619934No Hit
CCTTAAGACTGCTTACATAAGCATGAAAAGGGGGCTTTGTTCCACAGGAA590.10921476435526267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.10366147125245269No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAAGA250.002046405371.398332
AGAACAA1100.070.316542
GAACAAG850.069.998373
GAAAACA1950.061.024221
ATGGGCG300.004215140459.4986155
GTACAAA603.097357E-759.4986151
ACTACCT300.004215140459.4986155
CTACCTC300.004215140459.4986156
TCACTAC300.004215140459.4986153
CGTCACT300.004215140459.4986151
AGGGAAG300.004215140459.4986158
CAAGAAA401.7570263E-459.498614
ACATGGA752.2582753E-855.5320363
GTCACTA350.00775703150.998812
GAGAACA2650.049.3950771
TGGGAGA751.4449652E-647.598896
ACAAGAA505.2879145E-447.598893
AACAAGG1152.6557245E-1046.5641334
ACAAGGA653.4760094E-545.768165
ATGGGGA1352.5465852E-1144.0730485
Produced by FastQC (version 0.11.5)