##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940128_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54022 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31853689237718 33.0 31.0 34.0 30.0 34.0 2 32.33453037651327 33.0 31.0 34.0 30.0 34.0 3 32.365758394728076 34.0 31.0 34.0 30.0 34.0 4 35.97027137092296 37.0 35.0 37.0 35.0 37.0 5 36.02421235792825 37.0 35.0 37.0 35.0 37.0 6 36.14736588797157 37.0 36.0 37.0 35.0 37.0 7 36.097497315908335 37.0 36.0 37.0 35.0 37.0 8 36.13207582096183 37.0 36.0 37.0 35.0 37.0 9 37.81222464921699 39.0 38.0 39.0 35.0 39.0 10-11 37.85681759283255 39.0 38.0 39.0 35.0 39.0 12-13 37.84593313835104 39.0 38.0 39.0 35.0 39.0 14-15 38.84891340564955 40.0 38.0 41.0 35.0 41.0 16-17 38.91806116026804 40.0 38.0 41.0 35.5 41.0 18-19 39.179195512939174 40.5 38.5 41.0 35.0 41.0 20-21 39.19758616859798 41.0 39.0 41.0 35.0 41.0 22-23 39.03306986042723 41.0 38.5 41.0 35.0 41.0 24-25 38.86372218725704 41.0 38.0 41.0 35.0 41.0 26-27 38.452380511643405 40.5 37.0 41.0 35.0 41.0 28-29 38.10472585243049 40.0 36.5 41.0 34.0 41.0 30-31 37.240290992558585 39.0 35.5 41.0 33.5 41.0 32-33 36.262365332642254 37.0 35.0 41.0 33.0 41.0 34-35 35.74513161304654 37.0 35.0 41.0 32.0 41.0 36-37 35.23871755951279 36.5 35.0 41.0 31.0 41.0 38-39 34.667478064492244 35.5 35.0 40.0 27.5 41.0 40-41 34.232414571841105 36.0 35.0 40.0 24.0 41.0 42-43 33.58544666987524 36.0 35.0 40.0 16.0 41.0 44-45 32.72207619118137 36.0 34.0 41.0 2.0 41.0 46-47 31.027257413646293 36.0 32.0 41.0 2.0 41.0 48-49 29.484385990892598 36.0 24.0 41.0 2.0 41.0 50-51 28.7276665062382 35.0 19.0 40.0 2.0 41.0 52-53 28.520371330198806 35.0 14.0 40.0 2.0 41.0 54-55 28.197354781385364 35.0 7.5 40.0 2.0 41.0 56-57 27.898254414868017 35.0 4.0 40.0 2.0 41.0 58-59 27.628558735330053 35.0 2.0 40.0 2.0 41.0 60-61 27.07535818740513 35.0 2.0 39.5 2.0 41.0 62-63 26.61879419495761 35.0 2.0 39.0 2.0 41.0 64-65 26.119201436451817 35.0 2.0 39.0 2.0 41.0 66-67 25.44063529673096 35.0 2.0 38.0 2.0 41.0 68-69 24.745853541149902 35.0 2.0 37.0 2.0 40.5 70-71 23.980267298508018 35.0 2.0 37.0 2.0 40.0 72-73 23.523055421865166 34.5 2.0 36.0 2.0 39.0 74-75 23.276720595313023 34.0 2.0 36.0 2.0 39.0 76-77 23.02987671689312 34.0 2.0 35.0 2.0 39.0 78-79 22.765160490170672 34.0 2.0 35.0 2.0 37.0 80-81 22.53194069082966 34.0 2.0 35.0 2.0 37.0 82-83 22.315167894561476 34.0 2.0 35.0 2.0 36.5 84-85 22.12146903113546 33.5 2.0 35.0 2.0 36.0 86-87 21.869099626078263 33.0 2.0 35.0 2.0 36.0 88-89 21.672096553256083 33.0 2.0 35.0 2.0 35.5 90-91 21.520047388101144 33.0 2.0 35.0 2.0 35.0 92-93 21.366211913664806 33.0 2.0 35.0 2.0 35.0 94-95 21.225223057272963 33.0 2.0 35.0 2.0 35.0 96-97 21.078329199215133 33.0 2.0 35.0 2.0 35.0 98-99 20.93560956647292 33.0 2.0 35.0 2.0 35.0 100-101 20.799896338528747 32.5 2.0 35.0 2.0 35.0 102-103 20.163331605642146 30.0 2.0 34.5 2.0 35.0 104-105 20.468068564658843 31.5 2.0 35.0 2.0 35.0 106-107 20.363749953722557 32.0 2.0 35.0 2.0 35.0 108-109 20.172762948428417 31.5 2.0 35.0 2.0 35.0 110-111 19.95033504868387 31.0 2.0 35.0 2.0 35.0 112-113 19.662702232423825 30.5 2.0 35.0 2.0 35.0 114-115 19.323155381141017 29.5 2.0 35.0 2.0 35.0 116-117 18.957304431527895 29.0 2.0 35.0 2.0 35.0 118-119 18.712894746584723 27.0 2.0 35.0 2.0 35.0 120-121 18.47092851060679 25.0 2.0 35.0 2.0 35.0 122-123 18.1517807559883 22.0 2.0 35.0 2.0 35.0 124-125 17.000740439080374 2.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11056408870792467 1101 2 0.3863497992181735 1101 3 0.29162778494396235 1101 4 0.30758831467733927 1101 5 0.38927146260164847 1101 6 0.28165363745356586 1101 7 -0.013617863072610703 1101 8 0.010341398516246159 1101 9 0.36097593088499735 1101 10-11 0.16811039523941673 1101 12-13 0.3144713197828892 1101 14-15 0.0077785903397895595 1101 16-17 -0.13913909356131882 1101 18-19 0.06506756026814031 1101 20-21 0.2695930410263614 1101 22-23 0.3519228113080288 1101 24-25 0.48141073048425653 1101 26-27 0.43560551401411374 1101 28-29 0.5849442746051068 1101 30-31 0.9325999696938041 1101 32-33 1.0803203834781385 1101 34-35 0.4865686407151628 1101 36-37 0.24718970449113442 1101 38-39 0.4934187050192307 1101 40-41 0.035601032188154136 1101 42-43 0.5498198315679872 1101 44-45 0.7868088392522665 1101 46-47 -1.0387803474766066 1101 48-49 -2.3899285497004072 1101 50-51 -2.2613363772684743 1101 52-53 -2.007486123260179 1101 54-55 -1.6035281951666036 1101 56-57 -1.3259050831695767 1101 58-59 -0.8645393424539094 1101 60-61 -0.2913445304387636 1101 62-63 0.336249525106858 1101 64-65 -0.38014757328729587 1101 66-67 -1.104794894261662 1101 68-69 -0.6425789681067897 1101 70-71 -1.1003186684803907 1101 72-73 -1.2288632748465922 1101 74-75 -1.2042567547482577 1101 76-77 -1.3790856246736567 1101 78-79 -1.4989089078430986 1101 80-81 -1.4297357067521688 1101 82-83 -1.0733072132962498 1101 84-85 -1.4065209226573607 1101 86-87 -0.9509168340183045 1101 88-89 -1.0962861424714596 1101 90-91 -1.568696660938837 1101 92-93 -1.7237859989614215 1101 94-95 -1.7173264852347891 1101 96-97 -1.8506052141337115 1101 98-99 -1.9081342186229158 1101 100-101 -1.9405559916965878 1101 102-103 -1.7029074107082671 1101 104-105 -1.526461393674115 1101 106-107 -1.472746666649833 1101 108-109 -1.834170970009371 1101 110-111 -1.2428077886202722 1101 112-113 -1.0043493641150292 1101 114-115 -0.5148819397728559 1101 116-117 -0.43119521414546114 1101 118-119 -0.4327242871695134 1101 120-121 -1.1099657705336732 1101 122-123 -0.676623899471533 1101 124-125 -0.5313851286963462 1102 1 0.1934212315650683 1102 2 -0.017459724591354586 1102 3 -0.13503888172270706 1102 4 0.36092164801066673 1102 5 0.3397476530778434 1102 6 0.08355839935832421 1102 7 0.13114404168928928 1102 8 0.08748425565909912 1102 9 0.5562140261230937 1102 10-11 -0.14046103333200932 1102 12-13 0.2535189388305028 1102 14-15 0.18968335224455046 1102 16-17 0.3346704302482095 1102 18-19 0.2850675602681392 1102 20-21 -0.244216482783159 1102 22-23 0.2643037636889787 1102 24-25 0.4561726352461619 1102 26-27 0.25655789496649106 1102 28-29 0.49208713174796515 1102 30-31 0.6954571125509474 1102 32-33 1.4350822882400465 1102 34-35 1.1165686407151583 1102 36-37 1.3790944663958982 1102 38-39 2.082942514543042 1102 40-41 1.638458175045301 1102 42-43 0.8960103077584662 1102 44-45 1.946332648776071 1102 46-47 2.4102672715710085 1102 48-49 3.5010238312519775 1102 50-51 2.130092194160099 1102 52-53 1.7967995910255325 1102 54-55 1.9450432334048244 1102 56-57 2.039333012068518 1102 58-59 1.7740320861175185 1102 60-61 2.244369755275521 1102 62-63 1.9333923822497177 1102 64-65 1.3722333790936574 1102 66-67 2.1433003438335767 1102 68-69 1.7731353176074975 1102 70-71 1.9782527600910385 1102 72-73 2.5773272013438806 1102 74-75 2.1890765785850768 1102 76-77 2.433771518183484 1102 78-79 2.3310910921568997 1102 80-81 2.2950261980097366 1102 82-83 2.236216596227557 1102 84-85 2.3953838392473976 1102 86-87 1.9519403088388394 1102 88-89 2.5032376670523497 1102 90-91 2.69273191048973 1102 92-93 3.095261620086198 1102 94-95 3.2969592290509304 1102 96-97 3.080347166818669 1102 98-99 2.969961019472322 1102 100-101 3.1137297225891274 1102 102-103 3.2842354464345895 1102 104-105 3.087824320611599 1102 106-107 2.5524914285882616 1102 108-109 2.632495696657294 1102 110-111 2.94100173518925 1102 112-113 2.2170792073135424 1102 114-115 2.4103561554652373 1102 116-117 2.3649952620450136 1102 118-119 2.3658471414019147 1102 120-121 2.4467008961329952 1102 122-123 2.225757052909419 1102 124-125 2.043852966541749 1103 1 0.25342123156507057 1103 2 0.29677450964288 1103 3 0.09703319034936442 1103 4 -0.13565492856590566 1103 5 -0.10223432890413875 1103 6 0.11292776872769394 1103 7 0.4172701678154169 1103 8 0.3999166880915368 1103 9 0.5192770891861542 1103 10-11 0.2935930207220352 1103 12-13 -0.06332790801634047 1103 14-15 0.4660797486409436 1103 16-17 0.3369226825004574 1103 18-19 0.06641891161949331 1103 20-21 0.4421799136132307 1103 22-23 0.42142088080610307 1103 24-25 0.49202849110201896 1103 26-27 0.9188101472187498 1103 28-29 1.0515465912074191 1103 30-31 1.1646463017401416 1103 32-33 1.0625597657175163 1103 34-35 1.097379451525967 1103 36-37 1.3095449168463489 1103 38-39 2.055194766795289 1103 40-41 2.5919716885588144 1103 42-43 2.830514812262969 1103 44-45 3.025611928055355 1103 46-47 1.0131501544538963 1103 48-49 0.3527355429636856 1103 50-51 0.9263084103763148 1103 52-53 1.4513941856201313 1103 54-55 1.1101783685399624 1103 56-57 1.5526663454018532 1103 58-59 0.9221401942256264 1103 60-61 0.9795048904106594 1103 62-63 1.5717707606280946 1103 64-65 1.3407018475621264 1103 66-67 1.8558228663560996 1103 68-69 2.133675858148038 1103 70-71 2.1755500573883353 1103 72-73 1.5058857599024407 1103 74-75 1.7781756776841782 1103 76-77 1.4151228695348372 1103 78-79 1.279469470535279 1103 80-81 1.686287459271 1103 82-83 1.414144524155489 1103 84-85 1.443672127535688 1103 86-87 1.4378862547847824 1103 88-89 1.473598027412713 1103 90-91 0.9148940726518937 1103 92-93 0.9281445029690829 1103 94-95 1.569932202023903 1103 96-97 1.2677345542060579 1103 98-99 0.9105916501029547 1103 100-101 0.8453513442107479 1103 102-103 0.9555867977859442 1103 104-105 0.9770135098007913 1103 106-107 0.25041935651618985 1103 108-109 0.5658290299906277 1103 110-111 0.49676750095501987 1103 112-113 1.0562683965027304 1103 114-115 1.3150408401499227 1103 116-117 1.818418685468437 1103 118-119 1.606657952212725 1103 120-121 1.9630072024393037 1103 122-123 2.1726038997562647 1103 124-125 2.2025917052804864 1104 1 -0.4465787684349287 1104 2 -0.3082930579246863 1104 3 -0.20837221505603765 1104 4 -0.2799116853226664 1104 5 -0.16608568025549175 1104 6 -0.0897749339750078 1104 7 -0.1296892916440413 1104 8 0.30498425565910026 1104 9 0.2770473594564251 1104 10-11 0.05203896666798613 1104 12-13 -0.0935643945028275 1104 14-15 -0.001149981088779839 1104 16-17 0.14300376358153954 1104 18-19 0.35881756026813605 1104 20-21 0.18120018388350445 1104 22-23 0.05638709702231637 1104 24-25 0.3215893019128231 1104 26-27 0.5498912282998276 1104 28-29 0.8733371317479595 1104 30-31 0.11920711255094574 1104 32-33 -0.07950104509328781 1104 34-35 -0.25718135928484287 1104 36-37 -0.3242388669374421 1104 38-39 0.0800258478763709 1104 40-41 0.12220817504530146 1104 42-43 0.15517697442512457 1104 44-45 0.8046659821094053 1104 46-47 0.8344339382376802 1104 48-49 0.3743571645853052 1104 50-51 0.7038421941600959 1104 52-53 1.1880495910255355 1104 54-55 0.543793233404827 1104 56-57 -0.02233365459814607 1104 58-59 0.40153208611751623 1104 60-61 0.4381197552755225 1104 62-63 0.8871423822497171 1104 64-65 0.7555667124269903 1104 66-67 0.1291336771669087 1104 68-69 0.6993853176075007 1104 70-71 0.9782527600910385 1104 72-73 0.7202438680105487 1104 74-75 0.7019932452517459 1104 76-77 1.0637715181834864 1104 78-79 0.9948410921569 1104 80-81 1.2166928646764035 1104 82-83 1.4945499295608897 1104 84-85 1.5345505059140656 1104 86-87 1.3794403088388378 1104 88-89 1.6090710003856863 1104 90-91 1.371481910489731 1104 92-93 1.111928286752864 1104 94-95 1.4344592290509262 1104 96-97 1.5199305001520003 1104 98-99 1.8445443528056558 1104 100-101 1.4987297225891254 1104 102-103 1.5979854464345884 1104 104-105 1.400324320611599 1104 106-107 2.5620747619215933 1104 108-109 2.478329029990629 1104 110-111 2.320585068522586 1104 112-113 1.742079207313541 1104 114-115 2.1261894887985733 1104 116-117 1.8929119287116798 1104 118-119 2.345847141401915 1104 120-121 1.5150342294663268 1104 122-123 1.0715903862427538 1104 124-125 1.3096862998750822 1105 1 -0.04657876843492659 1105 2 0.19587360874198367 1105 3 0.5849611182772918 1105 4 0.400921648010673 1105 5 0.3664143197445071 1105 6 0.4702250660249945 1105 7 0.3311440416892921 1105 8 0.5008175889924331 1105 9 0.3162140261230917 1105 10-11 0.5595389666679864 1105 12-13 0.5001856054971725 1105 14-15 0.676350018911215 1105 16-17 0.7746704302482073 1105 18-19 0.4917342269348097 1105 20-21 0.5157835172168319 1105 22-23 0.6909704303556481 1105 24-25 0.822839301912822 1105 26-27 0.9432245616331585 1105 28-29 0.49875379841462575 1105 30-31 0.14212377921761998 1105 32-33 0.07508228824004703 1105 34-35 -0.2634313592848372 1105 36-37 -0.027572200270775227 1105 38-39 0.18960918120970405 1105 40-41 0.6117915083786301 1105 42-43 0.9626769744251291 1105 44-45 1.6063326487760818 1105 46-47 1.9369339382376793 1105 48-49 2.4076904979186367 1105 50-51 2.5034255274934303 1105 52-53 1.6634662576921997 1105 54-55 1.9717099000714953 1105 56-57 1.445999678735184 1105 58-59 1.4073654194508514 1105 60-61 1.777703088608856 1105 62-63 1.5267257155830514 1105 64-65 2.018900045760322 1105 66-67 2.943300343833574 1105 68-69 1.8331353176074998 1105 70-71 2.338252760091038 1105 72-73 2.3906605346772167 1105 74-75 1.9424099119184106 1105 76-77 1.4337715181834874 1105 78-79 1.0710910921569017 1105 80-81 0.8483595313430712 1105 82-83 0.8362165962275583 1105 84-85 1.0887171725807328 1105 86-87 1.1652736421721706 1105 88-89 1.1899043337190172 1105 90-91 1.346065243823066 1105 92-93 1.188594953419532 1105 94-95 0.8769592290509287 1105 96-97 1.1736805001520025 1105 98-99 1.1499610194723253 1105 100-101 1.4603963892557914 1105 102-103 1.6642354464345885 1105 104-105 1.3611576539449324 1105 106-107 1.79249142858826 1105 108-109 1.039162363323964 1105 110-111 0.6810017351892519 1105 112-113 0.9304125406468735 1105 114-115 1.1503561554652393 1105 116-117 0.6516619287116825 1105 118-119 0.7991804747352482 1105 120-121 1.180034229466326 1105 122-123 1.372423719576087 1105 124-125 1.710519633208417 1106 1 0.22778020592404147 1106 2 0.165104377972753 1106 3 0.12752522084139883 1106 4 0.2742549813440007 1106 5 0.2515425248727112 1106 6 -0.1154159596160369 1106 7 0.09883634938159958 1106 8 -0.6094388212639785 1106 9 -0.09506802515895885 1106 10-11 0.008769735898752629 1106 12-13 0.09762150293307315 1106 14-15 -0.07775254519134478 1106 16-17 -0.896098800521024 1106 18-19 -0.246470901270321 1106 20-21 0.23809120952452645 1106 22-23 -0.368773159387942 1106 24-25 -0.27408377501025427 1106 26-27 0.09604507445367716 1106 28-29 0.0023435420043682598 1106 30-31 -0.306594169500336 1106 32-33 -0.011071557913801655 1106 34-35 0.7224660766125908 1106 36-37 0.30140215870358134 1106 38-39 0.3990963606968805 1106 40-41 0.3861504827376052 1106 42-43 0.678574410322561 1106 44-45 0.5027429051863308 1106 46-47 0.08539547669921888 1106 48-49 0.18589562612376653 1106 50-51 0.556246040313944 1106 52-53 0.2670560012819436 1106 54-55 0.7314534898150811 1106 56-57 0.5718971146326233 1106 58-59 0.36531413739956875 1106 60-61 1.4405236014293692 1106 62-63 1.1677513566086866 1106 64-65 1.2760795329398107 1106 66-67 1.7371464976797277 1106 68-69 1.6380071124792934 1106 70-71 1.076970708808986 1106 72-73 1.5498913039079873 1106 74-75 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0.7986561301659592 2216 120-121 0.6220567013764402 2216 122-123 0.6029106109618532 2216 124-125 0.33089416504362035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 13.0 6 44.0 7 97.0 8 260.0 9 434.0 10 837.0 11 1033.0 12 1287.0 13 1979.0 14 3467.0 15 3450.0 16 1164.0 17 794.0 18 746.0 19 912.0 20 1008.0 21 1308.0 22 1286.0 23 484.0 24 467.0 25 530.0 26 554.0 27 584.0 28 694.0 29 770.0 30 907.0 31 977.0 32 1306.0 33 1496.0 34 2244.0 35 4603.0 36 4657.0 37 8581.0 38 4951.0 39 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.82359149062228 18.007442928292384 17.95189868730444 16.217066893780895 2 15.77349549249366 22.74485848096111 43.21282464226875 18.268821384276485 3 16.439598682018435 34.46558809373958 36.00385028321795 13.090962941024028 4 9.900513181540285 22.71523056116494 35.53735850454823 31.84689775274654 5 7.6154159416534 38.625004627744254 34.374884306393696 19.384695124208655 6 20.08072280234022 41.277864178330745 17.053617714581947 21.587795304747093 7 15.460367998222946 41.99955573655178 21.215430750435008 21.324645514790273 8 24.034652548961535 31.048461737810523 21.791122135426306 23.125763577801635 9 25.843175002776647 13.762911406464035 20.241753359742326 40.15216023101699 10-11 21.481063270519417 28.392136536966422 25.738587982673728 24.388212209840436 12-13 29.370441671911447 27.75535892784421 27.428640183628893 15.445559216615454 14-15 23.9596830920736 25.077746103439342 33.822331642664096 17.14023916182296 16-17 14.494094998334011 28.91044389322868 34.234200881122504 22.361260227314798 18-19 15.057754248269223 18.60723408981526 48.65517752026952 17.679834141645994 20-21 16.695050164747695 17.148569101477175 50.290622339047054 15.865758394728074 22-23 14.934656251156936 19.85765058679797 50.56458479878568 14.643108363259413 24-25 15.601195820182706 19.65606286385976 51.264774210268136 13.4779671056894 26-27 15.987930842989892 18.99596460701196 51.2828107067491 13.733293843249047 28-29 14.377741989226411 17.5348475592825 52.84333870161604 15.244071749875049 30-31 14.828939573459715 18.492668838862556 52.93153880331754 13.74685278436019 32-33 12.655029432453446 17.668727555440373 54.30935544778054 15.366887564325646 34-35 12.822553774388211 17.93713672207619 52.7525822812928 16.48772722224279 36-37 14.213653696642108 18.26570656399245 52.40179926696531 15.118840472400134 38-39 14.954231185731606 16.653554603259813 51.715520672324914 16.676693538683672 40-41 14.447951278657573 16.73778032820266 52.403209833122 16.41105856001777 42-43 15.727851616008293 16.830180296916073 50.99496501425346 16.447003072822184 44-45 13.835075020594797 19.340596277201332 48.643520275461185 18.18080842674269 46-47 16.752128841169935 17.86653091447612 44.61032950758978 20.77101073676416 48-49 17.105993854355635 18.71737440302099 41.67931583428973 22.497315908333643 50-51 16.04483483122148 20.62828000481299 40.78914486167288 22.537740302292647 52-53 15.884269371737442 21.345933138351043 41.228573544111654 21.54122394579986 54-55 15.2815187108359 21.4274474958117 39.48296448504707 23.808069308305335 56-57 16.85239346932731 22.19928917848284 36.252822923993925 24.69549442819592 58-59 14.76013735526328 23.200451680380596 38.69549522866319 23.34391573569293 60-61 17.291103624449295 24.777868275887602 35.37077486949761 22.56025323016549 62-63 16.43943390812577 26.15351863678857 35.18636788567092 22.22067956941475 64-65 16.43295476633438 25.772174862781032 35.96201371726877 21.832856653615824 66-67 16.546189942893108 25.606471497459342 36.12265486889479 21.724683690752755 68-69 16.37681293559046 25.460233425580558 36.45678109641531 21.70617254241367 70-71 16.7977933689998 24.598752290860624 36.52696273533386 22.076491604805717 72-73 16.75475051137994 23.244879258799898 36.31214076137763 23.688229468442536 74-75 16.119358779756396 23.05819851171745 36.62674465958313 24.195698048943022 76-77 15.656238430211033 22.47593483894854 37.8748611625324 23.992965568308033 78-79 16.059346741575116 21.63490462130818 38.06077209999722 24.24497653711948 80-81 15.720592726969818 22.69744453597179 37.23702599890784 24.34493673815055 82-83 16.634271857240705 22.041428333425888 36.38862664519353 24.93567316413987 84-85 16.52197252971012 21.957720928510607 35.672503794750284 25.847802747028986 86-87 16.880628650234634 21.846336113142232 35.263464795771974 26.00957044085116 88-89 17.412391936170607 21.0638849293767 36.054906606934594 25.468816527518097 90-91 17.318461609663537 21.349562641736476 35.44499467765076 25.88698107094923 92-93 17.1694358309807 21.926227611422224 34.34442541768871 26.559911139908365 94-95 17.449577459574034 21.410257596934386 34.52243212973333 26.61773281375825 96-97 17.12421325435024 21.390225842280636 33.808774527952615 27.676786375416512 98-99 16.77033581398793 22.05375985782517 33.63601021881595 27.539894109370948 100-101 17.95109128269683 22.244025250374868 33.39658268387049 26.408300783057815 102-103 17.83859830990087 22.313751261095323 33.237382104941645 26.610268324062165 104-105 17.49875048592214 22.68654782399437 33.18894503989189 26.62575665019159 106-107 17.513235348561697 23.829180704157565 32.43215726926067 26.225426678020064 108-109 16.98647635444725 23.712199050290188 32.13462553108772 27.166699064174836 110-111 16.47322337609448 23.80648266414913 32.506802909979456 27.213491049776938 112-113 17.328927516915186 23.765492090819055 32.47808661686983 26.42749377539592 114-115 16.885713227944173 24.29195512939173 32.38217763133538 26.44015401132872 116-117 16.32205397800896 24.55388545407427 32.630224723260895 26.493835844655884 118-119 16.232876712328768 24.92965568308034 32.55831173639393 26.279155868196963 120-121 16.223945799859315 25.201769649402095 32.10266187849395 26.471622672244642 122-123 15.962310953147849 25.42992539938172 31.57198126654449 27.035782380925937 124-125 15.43632239248183 26.16452941993426 31.552242951715197 26.84690523586871 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 976.0 1 600.0 2 314.5 3 343.5 4 170.5 5 112.5 6 267.5 7 540.0 8 861.0 9 895.5 10 537.0 11 239.0 12 186.0 13 199.0 14 214.0 15 218.0 16 232.0 17 244.5 18 238.0 19 240.0 20 272.0 21 280.5 22 287.0 23 324.5 24 342.0 25 376.0 26 443.0 27 504.0 28 579.0 29 684.0 30 781.5 31 885.5 32 966.5 33 1037.0 34 1158.5 35 1261.0 36 1341.5 37 1393.0 38 1422.0 39 1450.5 40 1480.5 41 1603.5 42 1688.0 43 1761.0 44 1924.0 45 1806.5 46 1663.5 47 1746.0 48 2131.5 49 2126.5 50 1977.0 51 2198.0 52 2061.0 53 1650.5 54 1166.5 55 817.0 56 615.5 57 494.5 58 457.0 59 396.0 60 388.5 61 387.5 62 270.5 63 126.0 64 27.5 65 19.5 66 13.0 67 14.0 68 15.5 69 12.5 70 11.5 71 9.0 72 5.5 73 4.5 74 7.5 75 5.5 76 3.0 77 2.5 78 0.5 79 0.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02036207471030321 2 0.0018510977009366554 3 0.0 4 0.0832993965421495 5 0.0 6 0.018510977009366554 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.255488504683277E-4 26-27 0.0 28-29 0.0018510977009366554 30-31 0.011106586205619932 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.255488504683277E-4 40-41 9.255488504683277E-4 42-43 0.0 44-45 0.006478841953278293 46-47 0.003702195401873311 48-49 0.0 50-51 0.002776646551404983 52-53 0.0 54-55 0.0046277442523416385 56-57 0.0 58-59 0.002776646551404983 60-61 0.0 62-63 0.0046277442523416385 64-65 0.0046277442523416385 66-67 9.255488504683277E-4 68-69 9.255488504683277E-4 70-71 0.005553293102809966 72-73 0.002776646551404983 74-75 0.0 76-77 0.003702195401873311 78-79 9.255488504683277E-4 80-81 9.255488504683277E-4 82-83 0.0018510977009366554 84-85 0.0 86-87 0.002776646551404983 88-89 0.005553293102809966 90-91 0.00832993965421495 92-93 0.00832993965421495 94-95 0.006478841953278293 96-97 0.003702195401873311 98-99 0.007404390803746622 100-101 0.005553293102809966 102-103 0.002776646551404983 104-105 0.0018510977009366554 106-107 0.0 108-109 0.010181037355151605 110-111 0.0018510977009366554 112-113 0.0046277442523416385 114-115 0.0 116-117 0.0 118-119 0.003702195401873311 120-121 0.0 122-123 0.0018510977009366554 124-125 0.03609640516826478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 54022.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.16430343193514 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6328913864255 33.15501092147643 2 10.00965844235666 8.441005516271149 3 3.850206339450347 4.870238051164341 4 2.138027921678813 3.6059383214246044 5 1.2951093160066731 2.730369108881567 6 0.781455790675213 1.976972344600348 7 0.5312143296162964 1.567879752693347 8 0.42584950390727894 1.4364518159268445 9 0.3512160856967249 1.332790344674392 >10 1.7429098252699973 12.761467550257303 >50 0.11853542892264467 3.385657695013143 >100 0.09219422249539029 8.311428677205583 >500 0.013170603213627185 3.9002628558735335 >1k 0.017560804284836244 12.524527044537411 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2194 4.061308355855022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1817 3.363444522601903 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 1504 2.78405094220873 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1251 2.315723223871756 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 794 1.4697715745437043 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 768 1.4216430343193514 RNA PCR Primer, Index 27 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 545 1.0088482470104772 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 469 0.8681648217392914 RNA PCR Primer, Index 27 (96% over 25bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 448 0.8292917700196215 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 0.8200362815149383 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 321 0.5942023620006665 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 306 0.5664358964866165 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 301 0.5571804079819334 RNA PCR Primer, Index 27 (96% over 30bp) CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT 215 0.39798600570138093 RNA PCR Primer, Index 27 (96% over 28bp) GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 196 0.36281514938358445 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 183 0.3387508792714079 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 182 0.3368997815704713 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 165 0.30543112065454814 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 159 0.29432453444892825 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.2869201436451816 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 134 0.24804709192551183 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 126 0.23323831031801856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 126 0.23323831031801856 No Hit CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG 124 0.22953611491614528 RNA PCR Primer, Index 27 (100% over 24bp) TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC 118 0.21842952871052534 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 110 0.2036207471030321 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 106 0.1962163562992855 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 103 0.19066306319647552 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 92 0.1703009884861723 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 89 0.16474769538336234 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 89 0.16474769538336234 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 86 0.15919440228055237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 85 0.1573433045796157 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 80 0.14808781607493243 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 78 0.14438562067305913 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 73 0.13513013216837585 No Hit CCTTAAGACTGCTTACATAAGCATGAAAAGGGGGCTTTGTTCCACAGGAA 71 0.13142793676650255 No Hit CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA 69 0.12772574136462922 RNA PCR Primer, Index 27 (96% over 30bp) ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC 67 0.12402354596275592 TruSeq Adapter, Index 27 (96% over 25bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 67 0.12402354596275592 No Hit TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT 66 0.12217244826181926 RNA PCR Primer, Index 27 (95% over 22bp) CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 0.11661915515900928 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 0.11661915515900928 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 63 0.11661915515900928 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 62 0.11476805745807264 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 62 0.11476805745807264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 60 0.11106586205619934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 59 0.10921476435526267 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.10921476435526267 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 56 0.10366147125245269 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 56 0.10366147125245269 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 56 0.10366147125245269 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0018510977009366554 0.0 14-15 0.0 0.0 0.0 0.0018510977009366554 0.0 16-17 0.0 0.0 0.0 0.0018510977009366554 0.0 18-19 0.0 0.0 0.0 0.0018510977009366554 0.0 20-21 0.0 0.0 0.0 0.002776646551404983 0.0 22-23 0.0 0.0 0.0 0.017585428158898228 0.0 24-25 0.0 0.0 0.0 0.060160675280441306 0.0 26-27 0.0 0.0 0.0 0.09903372700011107 0.0 28-29 0.0 0.0 0.0 0.20269519825256377 0.0 30-31 0.0 0.0 0.0 0.44056125282292397 0.0 32-33 0.0 0.0 0.0 0.7478434711784088 0.0 34-35 0.0 0.0 0.0 1.1291695975713598 0.0 36-37 0.0 0.0 0.0 1.609529450964422 0.0 38-39 0.0 0.0 0.0 2.2972122468623892 0.0 40-41 0.0 0.0 0.0 3.2135056088260336 0.0 42-43 0.0 0.0 0.0 3.765132723705157 0.0 44-45 0.0 0.0 0.0 4.1566398874532595 0.0 46-47 0.0 0.0 0.0 4.5944244937247785 0.0 48-49 0.0 0.0 0.0 5.112731849987043 0.0 50-51 0.0 0.0 0.0 5.644922439006331 0.0 52-53 0.0 0.0 0.0 6.126207841249862 0.0 54-55 0.0 0.0 0.0 6.5399281774092035 0.0 56-57 0.0 0.0 0.0 6.9453185739143315 0.0 58-59 0.0 0.0 0.0 7.400688608344748 0.0 60-61 0.0 0.0 0.0 7.896782792195772 0.0 62-63 0.0 0.0 0.0 8.354003924327127 0.0 64-65 0.0 0.0 0.0 8.776054200140685 0.0 66-67 0.0 0.0 0.0 9.237903076524379 0.0 68-69 0.0 0.0 0.0 9.761763725889452 0.0 70-71 0.0 0.0 0.0 10.334678464329347 0.0 72-73 0.0 0.0 0.0 10.963126133797342 0.0 74-75 0.0 0.0 0.0 11.499018918218503 0.0 76-77 0.0 0.0 0.0 12.000666395172338 0.0 78-79 0.0 0.0 0.0 12.526378142238347 0.0 80-81 0.0 0.0 0.0 13.113176113435268 0.0 82-83 0.0 0.0 0.0 13.722187257043426 0.0 84-85 0.0 0.0 0.0 14.399689015586244 0.0 86-87 0.0 0.0 0.0 15.016104549998149 0.0 88-89 0.0 0.0 0.0 15.621413498204436 0.0 90-91 0.0 0.0 0.0 16.308170745251935 0.0 92-93 0.0 0.0 0.0 16.96808707563585 0.0 94-95 0.0 0.0 0.0 17.66687645773944 0.0 96-97 0.0 0.0 0.0 18.29254748065603 0.0 98-99 0.0 0.0 0.0 19.026507719077415 0.0 100-101 0.0 0.0 0.0 19.74751027359224 0.0 102-103 0.0 0.0 0.0 20.506460330976267 0.0 104-105 0.0 0.0 0.0 21.283921365369665 0.0 106-107 0.0 0.0 0.0 21.99474288252934 0.0 108-109 0.0 0.0 0.0 22.72037318129651 0.0 110-111 0.0 0.0 0.0 23.50061086224131 0.0 112-113 0.0 0.0 0.0 24.332679278812336 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCGC 15 2.6590584E-4 119.319405 3 TGTAGCG 15 2.6590584E-4 119.319405 2 CGCTGGC 15 2.688701E-4 118.98796 7 AGCGCTG 15 2.688701E-4 118.98796 5 GCGCTGG 15 2.688701E-4 118.98796 6 TAGCGCT 20 8.3480426E-4 89.489555 4 ACTGCAC 20 8.440947E-4 89.24097 8 ATACTGC 20 8.440947E-4 89.24097 6 TGGGACT 35 9.078387E-5 67.993126 8 CATGGGT 30 0.0041701873 59.659702 4 GTGTAGC 40 1.73365E-4 59.659702 1 CCGGAAG 15 0.004227874 59.49398 114-115 TACTGCA 30 0.0042164396 59.49398 7 CTGCACA 30 0.0042164396 59.49398 9 GGTATCA 935 0.0 56.78838 1 ATGGGAC 45 3.1454687E-4 52.88354 7 TTTAAGG 35 0.0076744426 51.136887 2 CACTATG 35 0.0077594174 50.994843 7 GTATCAA 1525 0.0 46.162918 1 TATCAAC 1930 0.0 36.47588 2 >>END_MODULE