FastQCFastQC Report
Fri 27 May 2016
SRR940127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614477
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.067.8109051
GTATCAA42050.063.974861
ATCAACG53450.049.218113
TCAACGC53950.048.5413254
CAACGCA55600.046.885255
TATCAAC56800.046.732982
AACGCAG57350.045.7559856
GTACATG120200.041.2455831
TACATGG124050.039.581672
ACATGGG124550.037.7514573
ACGCAGA69900.037.2808537
CGCAGAG70200.036.954328
CATGGGA85050.034.430334
GCAGAGT77100.031.7949879
ATGGGAG36450.029.0642685
CATGGGG47950.029.0454164
ATGGGAT34050.028.490985
ATATGAG8200.024.678313
TATCTCG1451.3378928E-424.6274915
AGAGTAC73500.024.4055710-11
Produced by FastQC (version 0.11.5)