FastQCFastQC Report
Fri 27 May 2016
SRR940127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614477
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63020.39034312659765363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50080.31019333195827503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31380.19436634897864757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38400.085.81
GTATCAA82550.063.4402921
ATCAACG109400.047.6449433
TCAACGC110800.047.0414664
TATCAAC111350.046.9725152
CAACGCA114350.045.6891755
AACGCAG117250.044.5591166
ACGCAGA135750.038.2603267
CGCAGAG136700.037.863878
TGGTATC12450.035.8865242
GCAGAGT147150.034.730199
GTACATG119550.034.0380361
TACATGG123950.032.2970052
ACATGGG121450.031.7345853
GAGTACT81400.029.23545312-13
TATTACG850.007168985428.033712
AGAGTAC144250.027.77768310-11
AGTACTT83950.027.603312-13
CAGAGTA149050.027.50182310-11
GTGGTAT17400.027.3926561
Produced by FastQC (version 0.11.5)