FastQCFastQC Report
Fri 27 May 2016
SRR940126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319201
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61061.912901275371944No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14060.4404748105425735No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12760.39974812108984614No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9830.3079564287079301No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG7460.23370854101334268No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7140.22368350976344056No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6930.21710458300569233No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT6400.20050062499804197No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC5580.17481148242016786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.16509973339682518No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.1503754687485315No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4780.1497489042954126No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC4510.14129028417830772No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG4180.13095197070184617No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4120.12907227734248952No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA4090.12813243066281121No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA4080.12781914843625178No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA4080.12781914843625178No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT4010.1256261728503357No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3920.12280663281130072No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3890.1218667861316224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.12155350390506295No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3840.12030037499882519No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3590.11246831933483918No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG3530.11058862597548252No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3440.10776908593644757No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG3440.10776908593644757No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT3330.10432298144429372No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT3310.10369641699117484No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3310.10369641699117484No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG3290.10306985253805596No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA3290.10306985253805596No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT3260.10213000585837764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.075.039711
GTATCAA9850.061.6289751
GTATGGT1450.061.5664751
GTACAAG1900.059.5142561
TGTAGGC401.7679378E-459.5142562
TAGGCAT805.693437E-1059.5142565
TGGTACT1700.048.996397
GAAAACA3800.048.5511051
GGTACTC1850.048.2472428
AGATCAG751.4650323E-647.6114045
GGGATGC505.3287164E-447.5964937
TACAAGG1152.728484E-1046.5763742
CATTCCG909.6883014E-846.2816169
TTCACCG653.5159734E-545.7658587
GTGGTAC1850.045.0307586
GAACAAA6550.044.522121
TACAAGA1501.8189894E-1243.6437872
CATGGAT558.509092E-443.28314
GTACATG22450.042.94571
CCTTTAG1151.4175384E-841.4012221
Produced by FastQC (version 0.11.5)