FastQCFastQC Report
Fri 27 May 2016
SRR940126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319201
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74212.324867403297609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56441.768164886701483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42721.3383416718619303No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.5332063496041679No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.49435935351079724No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.37907149413692315No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT10890.3411643447232308No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9170.28727980175500706No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.26347035253648954No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.23370854101334268No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT7450.23339525878678324No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6870.21522488964633568No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT6300.1973678027324476No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.19078887597469935No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.19016231152158045No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT6000.18796933593566437RNA PCR Primer, Index 17 (95% over 21bp)
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC5350.16760599120930073No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4580.1434832597642238No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG4470.14003715527206995No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT4240.13283166406120284No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4210.13189181738152447No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC4170.13063868847528673No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA4070.12750586620969231No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA3980.12468632617065736No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG3830.11998709277226575No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA3820.11967381054570632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3720.11654098828011189No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3710.11622770605355247No Hit
TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA3580.11215503710827973No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG3490.10933549706924478No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3440.10776908593644757No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA3420.10714252148332867No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3410.10682923925676925No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT3400.1065159570302098No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT3400.1065159570302098RNA PCR Primer, Index 17 (95% over 23bp)
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT3370.1055761103505315No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3350.1049495458974126No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3300.10338313476461541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13550.097.09261
GTATCAA34400.071.470161
CTACTCG359.148156E-567.991239
GTACAGC401.7656818E-459.529623
TCAACGC46300.053.3580864
ATCAACG46950.052.619373
CAACGCA47350.052.267815
ACATATA803.8200596E-852.0884172
AACGCAG47600.051.868316
TATCAAC48000.051.34432
ATTAACG255.330084E-447.60131566-67
ACGCAGA53700.045.9763767
CGCAGAG53850.045.737838
GTATGGT1451.8189894E-1245.1604041
GTACTAC558.524552E-443.2671476
GCAGAGT57000.042.8970959
GGATATG1800.039.6864171
CAGAGTC600.001303897739.6864134
ACCGCGT300.001306890439.67397742-43
AAACCGC300.001306890439.67397740-41
Produced by FastQC (version 0.11.5)