##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 319201 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.666802422298176 34.0 31.0 34.0 31.0 34.0 2 32.68170525781561 34.0 31.0 34.0 31.0 34.0 3 32.76447755489488 34.0 31.0 34.0 31.0 34.0 4 36.22797860908957 37.0 35.0 37.0 35.0 37.0 5 36.23824800047619 37.0 37.0 37.0 35.0 37.0 6 36.3243943471355 37.0 37.0 37.0 35.0 37.0 7 36.27193523829813 37.0 37.0 37.0 35.0 37.0 8 36.28167831554413 37.0 37.0 37.0 35.0 37.0 9 38.06336446314391 39.0 39.0 39.0 35.0 39.0 10-11 38.04986356559033 39.0 39.0 39.0 35.0 39.0 12-13 38.065269219081394 39.0 39.0 39.0 35.0 39.0 14-15 39.391168887315516 41.0 39.0 41.0 36.0 41.0 16-17 39.41558767046469 41.0 39.0 41.0 36.0 41.0 18-19 39.594904151302785 41.0 39.5 41.0 37.0 41.0 20-21 39.59779731266506 41.0 40.0 41.0 37.0 41.0 22-23 39.50139567231932 41.0 39.5 41.0 36.0 41.0 24-25 39.4521868665825 41.0 39.0 41.0 35.5 41.0 26-27 39.277857838791235 41.0 39.0 41.0 35.0 41.0 28-29 39.11317790357799 41.0 39.0 41.0 35.0 41.0 30-31 38.7168899846805 40.5 37.5 41.0 35.0 41.0 32-33 38.24889646335694 40.0 36.5 41.0 35.0 41.0 34-35 37.99146932497079 40.0 35.5 41.0 34.5 41.0 36-37 37.78512442003628 40.0 35.0 41.0 34.0 41.0 38-39 37.55268780486277 40.0 35.0 41.0 33.0 41.0 40-41 37.369080923931946 40.0 35.0 41.0 33.0 41.0 42-43 37.06897534782159 40.0 35.0 41.0 33.0 41.0 44-45 36.8110406922284 40.0 35.0 41.0 32.0 41.0 46-47 36.09485089332427 40.0 35.0 41.0 27.5 41.0 48-49 35.46665110698275 40.0 35.0 41.0 21.5 41.0 50-51 35.254510480856894 40.0 35.0 41.0 20.0 41.0 52-53 35.212270638249876 40.0 35.0 41.0 20.0 41.0 54-55 35.101815157220685 40.0 35.0 41.0 18.0 41.0 56-57 34.984155751391754 40.0 35.0 41.0 17.5 41.0 58-59 34.798421684142596 40.0 35.0 41.0 14.0 41.0 60-61 34.60243702244041 39.5 35.0 41.0 9.0 41.0 62-63 34.371947456304966 39.0 35.0 41.0 6.0 41.0 64-65 34.08028483620039 39.0 35.0 41.0 2.0 41.0 66-67 33.76042524929433 38.5 35.0 41.0 2.0 41.0 68-69 33.39479512908794 37.0 35.0 40.0 2.0 41.0 70-71 33.056263921478944 37.0 35.0 40.0 2.0 41.0 72-73 32.78166108502167 36.5 35.0 39.0 2.0 41.0 74-75 32.43803904123108 36.0 35.0 39.0 2.0 41.0 76-77 32.08625442902748 36.0 34.0 38.5 2.0 40.5 78-79 31.727524349861056 35.0 34.0 37.0 2.0 39.0 80-81 31.388729985181747 35.0 34.0 37.0 2.0 39.0 82-83 31.068138884276678 35.0 34.0 36.5 2.0 39.0 84-85 30.752043383322736 35.0 34.0 36.0 2.0 37.0 86-87 30.520217668491014 35.0 34.0 36.0 2.0 37.0 88-89 30.29329012127155 35.0 33.5 35.5 2.0 37.0 90-91 30.106013139056582 35.0 33.0 35.0 2.0 36.0 92-93 29.939857644556252 35.0 33.0 35.0 2.0 36.0 94-95 29.7818490543576 35.0 33.0 35.0 2.0 36.0 96-97 29.65995094000332 35.0 33.0 35.0 2.0 36.0 98-99 29.564520474559917 35.0 33.0 35.0 2.0 35.5 100-101 29.502542285268532 35.0 33.0 35.0 2.0 35.0 102-103 28.783297984655437 34.5 31.0 35.0 2.0 35.0 104-105 29.276314923825424 35.0 32.5 35.0 2.0 35.0 106-107 29.26902328000226 35.0 33.0 35.0 2.0 35.0 108-109 29.22073709042265 35.0 33.0 35.0 2.0 35.0 110-111 29.133871761053378 35.0 33.0 35.0 2.0 35.0 112-113 29.01820013095197 35.0 33.0 35.0 2.0 35.0 114-115 28.896422630254918 35.0 32.5 35.0 2.0 35.0 116-117 28.75737231399651 35.0 32.0 35.0 2.0 35.0 118-119 28.632961676185225 35.0 32.0 35.0 2.0 35.0 120-121 28.466369152978842 35.0 32.0 35.0 2.0 35.0 122-123 28.27590609051977 35.0 32.0 35.0 2.0 35.0 124-125 26.80969201224307 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1028517318936224 1101 2 0.16115077525210353 1101 3 0.1634747223176305 1101 4 -0.015243735039511819 1101 5 0.05638631482898404 1101 6 0.02863698386617841 1101 7 0.012103842104622231 1101 8 0.017235805399202775 1101 9 0.04479328913075875 1101 10-11 -0.04804386309988473 1101 12-13 0.007462924900139001 1101 14-15 0.1420884692438662 1101 16-17 0.18916800924396426 1101 18-19 0.032675036693149195 1101 20-21 0.12248541257180534 1101 22-23 0.23169891241570895 1101 24-25 0.18266465911872132 1101 26-27 0.26107409346082733 1101 28-29 0.3498582660850573 1101 30-31 0.2035795503820097 1101 32-33 0.2919347458499786 1101 34-35 0.34358609159127695 1101 36-37 0.4581044188844672 1101 38-39 0.5360470891060345 1101 40-41 0.7107351439653513 1101 42-43 0.7118954304608991 1101 44-45 0.5479453510663674 1101 46-47 0.7702411007573176 1101 48-49 0.870282235573157 1101 50-51 0.7102677593402973 1101 52-53 0.5409156336849037 1101 54-55 0.6802873181331179 1101 56-57 0.6658731451640207 1101 58-59 0.5537247872396804 1101 60-61 0.7044753447160801 1101 62-63 0.6433508413997515 1101 64-65 0.6578980505419096 1101 66-67 0.6376426495917613 1101 68-69 0.7134660571436129 1101 70-71 0.7168378520776599 1101 72-73 0.6800758088276808 1101 74-75 0.7039706602473146 1101 76-77 0.6609031161885994 1101 78-79 0.7099616332246583 1101 80-81 0.7090112940523312 1101 82-83 0.569307236453831 1101 84-85 0.6811274565708558 1101 86-87 0.542006492859727 1101 88-89 0.6208205348720313 1101 90-91 0.5451370968198503 1101 92-93 0.7054152140573109 1101 94-95 0.8823897997144989 1101 96-97 0.8395698519494452 1101 98-99 0.8217309544598699 1101 100-101 0.87116387118283 1101 102-103 0.9200942619318084 1101 104-105 0.9936071619844746 1101 106-107 0.8082495658215869 1101 108-109 0.89681222744181 1101 110-111 0.9395270859288303 1101 112-113 0.9248273874888113 1101 114-115 1.0444779690482697 1101 116-117 0.9508857394698005 1101 118-119 0.9581967835070877 1101 120-121 0.9885205896238318 1101 122-123 0.9764682918643821 1101 124-125 0.8509132687638647 1102 1 -0.05214986606099359 1102 2 -0.04291563878555138 1102 3 0.08537278803161286 1102 4 -0.05764279757279667 1102 5 -0.036888579021173484 1102 6 -0.11851180853668808 1102 7 0.093127750549435 1102 8 0.12338564788653628 1102 9 -0.008015306343224893 1102 10-11 0.12197850826476753 1102 12-13 0.038052341874987405 1102 14-15 -0.13039323037110506 1102 16-17 -0.03709475536971496 1102 18-19 0.07649703976731104 1102 20-21 0.08701082005023864 1102 22-23 0.10101666188686664 1102 24-25 0.14257121682010165 1102 26-27 0.1378717924061732 1102 28-29 0.21446585408669705 1102 30-31 -0.0742819297805255 1102 32-33 -0.013578958513200234 1102 34-35 0.19527307510951175 1102 36-37 0.2906159469856462 1102 38-39 0.3302685808347263 1102 40-41 0.6037787148612708 1102 42-43 0.7038067363702822 1102 44-45 0.5661544244048784 1102 46-47 0.013952007938982547 1102 48-49 -0.23799821307163427 1102 50-51 -0.33614140835362605 1102 52-53 -0.2577573030513065 1102 54-55 -0.26305377669407903 1102 56-57 -0.28171394336266076 1102 58-59 -0.27195358254223834 1102 60-61 -0.21830540069170468 1102 62-63 -0.15186746963831155 1102 64-65 -0.3539724694781796 1102 66-67 -0.5016076814131196 1102 68-69 -0.41468229391941236 1102 70-71 -0.41852412268987393 1102 72-73 -0.2951939411304707 1102 74-75 -0.12902786354698748 1102 76-77 -0.07780086652304874 1102 78-79 -0.08840693120334464 1102 80-81 -0.06571515133566663 1102 82-83 -0.014395148303204763 1102 84-85 0.10018725051080324 1102 86-87 0.011477950346524324 1102 88-89 0.23331653998548418 1102 90-91 0.3728623703744631 1102 92-93 0.226652791862481 1102 94-95 0.2702666506785967 1102 96-97 0.21169543596381146 1102 98-99 0.3544509775921654 1102 100-101 -0.04213567682429087 1102 102-103 -0.19964580411698307 1102 104-105 -0.16809062675070763 1102 106-107 -0.2607820736798523 1102 108-109 -0.5660771788800041 1102 110-111 -0.4627161379826603 1102 112-113 -0.4043664824529003 1102 114-115 -0.5297203903849876 1102 116-117 -0.5470825753729152 1102 118-119 -0.585632828874779 1102 120-121 -0.4245126326136095 1102 122-123 -0.29307621497638436 1102 124-125 -0.4802133653349614 1103 1 0.09546918155805173 1103 2 0.0644766514638846 1103 3 -0.06936644099332767 1103 4 -0.014468194398197909 1103 5 -0.11902009829555027 1103 6 0.1307398921435805 1103 7 0.016846571411115008 1103 8 0.15300016035818942 1103 9 0.23511394535745467 1103 10-11 8.673971536552472E-4 1103 12-13 -0.11641477830642089 1103 14-15 -0.0660621646114663 1103 16-17 0.1083700972380015 1103 18-19 0.09953558852014766 1103 20-21 0.07209018512961052 1103 22-23 0.22462210406374084 1103 24-25 0.17842155695615958 1103 26-27 0.19199877653316122 1103 28-29 0.3023116590753574 1103 30-31 0.4063983423283162 1103 32-33 0.3805253498768195 1103 34-35 0.6101483585562306 1103 36-37 0.47961821455933773 1103 38-39 0.7607674470478756 1103 40-41 0.5609215720041334 1103 42-43 0.6153033350097417 1103 44-45 0.4551340162416153 1103 46-47 0.4352672006827518 1103 48-49 0.5900063220757659 1103 50-51 0.48467491817699226 1103 52-53 0.6444875949078721 1103 54-55 0.6825244545984432 1103 56-57 1.033524151875433 1103 58-59 0.8377516328772643 1103 60-61 0.5221934655214469 1103 62-63 0.6796744804750645 1103 64-65 0.6814923831295303 1103 66-67 0.6658753117841485 1103 68-69 0.6323698602755954 1103 70-71 0.6137207752693001 1103 72-73 0.5124024307516137 1103 74-75 0.6838066035731885 1103 76-77 0.5789791788284191 1103 78-79 0.5989400075721605 1103 80-81 0.5947383634035646 1103 82-83 0.5992102938736643 1103 84-85 0.5768992686513918 1103 86-87 0.5469654786911953 1103 88-89 0.6323641590331022 1103 90-91 0.7495517127780928 1103 92-93 0.5200088009327786 1103 94-95 0.7067745871865334 1103 96-97 0.8805616491157373 1103 98-99 0.8234759209028226 1103 100-101 0.7464584274840966 1103 102-103 0.5343224498512704 1103 104-105 0.5295057451313774 1103 106-107 0.45096395806617906 1103 108-109 0.36140581431727625 1103 110-111 0.5177373767565712 1103 112-113 0.5827990504269174 1103 114-115 0.6036129429483452 1103 116-117 0.7693346581871765 1103 118-119 0.75745107135198 1103 120-121 0.5675735351868418 1103 122-123 0.531322877994139 1103 124-125 0.3898319861389581 1104 1 0.08802160382848001 1104 2 0.08374876522623254 1104 3 0.018211288580062046 1104 4 0.04974968942923397 1104 5 -0.18299213975132034 1104 6 0.06569289286664315 1104 7 -0.12030454934087942 1104 8 0.014571623361327113 1104 9 0.18652874410414455 1104 10-11 0.28286064853953263 1104 12-13 0.17851894066200202 1104 14-15 0.24125767149361366 1104 16-17 0.1746872821018215 1104 18-19 0.1619024239021627 1104 20-21 0.2034760612443307 1104 22-23 0.11522465889622424 1104 24-25 0.1232058423261222 1104 26-27 0.1159732282082544 1104 28-29 0.25329743878131694 1104 30-31 0.15098257949389904 1104 32-33 0.09643279745454691 1104 34-35 -0.01950698007959062 1104 36-37 -0.14020583942957643 1104 38-39 0.14287156852292782 1104 40-41 -0.047273173717734096 1104 42-43 -0.12435200362607901 1104 44-45 -0.08959001364510755 1104 46-47 -0.37353009987160846 1104 48-49 -0.6096441961213941 1104 50-51 -0.818101654491123 1104 52-53 -0.8509836029711266 1104 54-55 -0.8351906574767582 1104 56-57 -1.0946464916531298 1104 58-59 -0.9366084538906279 1104 60-61 -1.060800026886362 1104 62-63 -0.9363028634632329 1104 64-65 -0.8315131603749322 1104 66-67 -0.7767711090401406 1104 68-69 -0.5214623884590353 1104 70-71 -0.5775494103175873 1104 72-73 -0.5396221534855457 1104 74-75 -0.3766754402928001 1104 76-77 -0.3185768587709994 1104 78-79 -0.401344398308769 1104 80-81 -0.5837721210505258 1104 82-83 -0.5880396458708503 1104 84-85 -0.6259134573066731 1104 86-87 -0.6711232173801491 1104 88-89 -0.6624586898461438 1104 90-91 -0.5880428883306088 1104 92-93 -0.698099176893205 1104 94-95 -0.7032037208394968 1104 96-97 -0.6295202983414789 1104 98-99 -0.7339499648527834 1104 100-101 -0.5815796539813185 1104 102-103 -0.48942303098748496 1104 104-105 -0.5366451345986754 1104 106-107 -0.6219017927171429 1104 108-109 -0.6303464151356053 1104 110-111 -0.41388952129115 1104 112-113 -0.390992224579108 1104 114-115 -0.4099324896860246 1104 116-117 -0.4618198368718751 1104 118-119 -0.5343541829261582 1104 120-121 -0.3133351175005501 1104 122-123 -0.2584674329241565 1104 124-125 -0.11605135924547128 1105 1 -0.05751095089227931 1105 2 -0.0220869982785743 1105 3 -0.0695430414348337 1105 4 -0.04460800308105206 1105 5 -0.16186042206302886 1105 6 0.10787013496918973 1105 7 -0.029246143820678583 1105 8 -0.07285708761826015 1105 9 -0.1400001090943448 1105 10-11 0.11645974446786056 1105 12-13 0.003070137072533896 1105 14-15 -0.055120295590128876 1105 16-17 -0.050827842423522895 1105 18-19 0.006570172773272986 1105 20-21 0.10514390109722882 1105 22-23 -0.07375170020410593 1105 24-25 0.07268940095909926 1105 26-27 0.1315646337811316 1105 28-29 0.2675360888031051 1105 30-31 -0.11863477025445945 1105 32-33 0.06507281124547859 1105 34-35 0.11931686481082693 1105 36-37 0.24808767739965987 1105 38-39 0.5752266081957771 1105 40-41 0.5433351113713059 1105 42-43 0.3985778015292354 1105 44-45 0.5103510876176287 1105 46-47 0.2504254052449255 1105 48-49 0.1224934449602344 1105 50-51 -0.03263192509011503 1105 52-53 -0.07891932194274887 1105 54-55 0.03351783208188408 1105 56-57 -0.19309836468086417 1105 58-59 -0.3091357843412794 1105 60-61 -0.1234577486065831 1105 62-63 -0.33084060414597616 1105 64-65 -0.26542446749592585 1105 66-67 -0.28419091338140845 1105 68-69 -0.1275639145588343 1105 70-71 0.056119597933026455 1105 72-73 -0.07247615644484995 1105 74-75 -0.26765770842091996 1105 76-77 -0.18397887856671957 1105 78-79 -0.20156036034542169 1105 80-81 -0.20014022379290708 1105 82-83 -0.335962627391968 1105 84-85 -0.27098152605059767 1105 86-87 -0.28478580902035944 1105 88-89 -0.30657623831788783 1105 90-91 -0.3732444608790111 1105 92-93 -0.04815161113491939 1105 94-95 -0.1806426313565126 1105 96-97 -0.11982834352590999 1105 98-99 0.1298335367968626 1105 100-101 0.06600956802861546 1105 102-103 0.20884783616473612 1105 104-105 0.21062421459421898 1105 106-107 0.2507284908108396 1105 108-109 0.19732928745928646 1105 110-111 0.2549559198029314 1105 112-113 0.12609559200160803 1105 114-115 0.1674834359579087 1105 116-117 0.02065180314670556 1105 118-119 0.058260490042194135 1105 120-121 -0.026606008595347674 1105 122-123 -0.1165358416526594 1105 124-125 0.32668996995058563 1106 1 0.08248672541770219 1106 2 0.002488347370317001 1106 3 -0.044805037484557886 1106 4 0.08143823835034425 1106 5 0.006476977727842836 1106 6 0.08372234828393488 1106 7 0.09614481702514865 1106 8 -0.0658302489985303 1106 9 0.05885663541593544 1106 10-11 -0.059425000507161485 1106 12-13 0.08159691760000953 1106 14-15 0.07668637340022855 1106 16-17 -0.03051879165089133 1106 18-19 0.06351219670727204 1106 20-21 0.12179778746878611 1106 22-23 0.012984677163153435 1106 24-25 0.09777828210235384 1106 26-27 0.001530940275848991 1106 28-29 0.15716546024495415 1106 30-31 0.2625386932055136 1106 32-33 0.2166657007540138 1106 34-35 0.2156454345796206 1106 36-37 0.09254219116752438 1106 38-39 0.17813586810050452 1106 40-41 0.11940110416787064 1106 42-43 0.37290567419103127 1106 44-45 0.4641398641948271 1106 46-47 0.2543315281681302 1106 48-49 0.4513513513155303 1106 50-51 0.5425696550190935 1106 52-53 0.7539027995862426 1106 54-55 0.7428753317914243 1106 56-57 0.7044013448578852 1106 58-59 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-0.9376881514077269 2216 40-41 -0.8140677029851418 2216 42-43 -1.0320668923604899 2216 44-45 -0.9770367259291319 2216 46-47 -0.7400009245853809 2216 48-49 -0.4571408250713844 2216 50-51 -0.41501620151413476 2216 52-53 -0.2305617401414537 2216 54-55 -0.2010019289279441 2216 56-57 -0.24953891118762783 2216 58-59 -0.3470746219489911 2216 60-61 -0.3704320171040294 2216 62-63 -0.22374036293977184 2216 64-65 -0.18744369580655018 2216 66-67 -0.06536020945137011 2216 68-69 -0.02239204448630261 2216 70-71 -0.17189484034631164 2216 72-73 0.2227627911119754 2216 74-75 0.20478043454702544 2216 76-77 0.23814262799187702 2216 78-79 0.1606431392752974 2216 80-81 -0.02073139206619601 2216 82-83 0.08307987774324843 2216 84-85 0.014724236476361341 2216 86-87 -1.8166845595146697E-4 2216 88-89 -0.08360752693858231 2216 90-91 -0.193571410345033 2216 92-93 -0.1502700493460729 2216 94-95 -0.5422601618482155 2216 96-97 -0.49299905444496517 2216 98-99 -0.6256617882348863 2216 100-101 -0.6740992730736046 2216 102-103 -0.6612502657214456 2216 104-105 -0.6627894071637748 2216 106-107 -0.4631630260608084 2216 108-109 -0.7030815301700706 2216 110-111 -0.6825328935136987 2216 112-113 -0.8433871357592686 2216 114-115 -0.9330880437526403 2216 116-117 -1.0115061826536653 2216 118-119 -1.158486294585387 2216 120-121 -1.0515427239294155 2216 122-123 -1.0675445608733014 2216 124-125 -1.3190011326941615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 8.0 5 49.0 6 116.0 7 249.0 8 441.0 9 853.0 10 1738.0 11 2315.0 12 2762.0 13 4114.0 14 7629.0 15 7779.0 16 2340.0 17 1626.0 18 1403.0 19 1657.0 20 1698.0 21 1923.0 22 1993.0 23 1450.0 24 1619.0 25 1845.0 26 2198.0 27 2384.0 28 2792.0 29 3110.0 30 3956.0 31 4873.0 32 6002.0 33 7629.0 34 10946.0 35 21939.0 36 27906.0 37 100989.0 38 78563.0 39 301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04966739048013 22.269640881987073 16.051111884014375 18.629579843518425 2 14.548741212295893 24.24999060138598 42.357360367924414 18.843907818393717 3 14.772428225917619 34.206569004624114 33.239451622200775 17.781551147257485 4 9.287026759229645 21.054314902263275 42.59496171429647 27.063696624210614 5 8.650933140345437 40.80785485806304 36.858808675683214 13.682403325908313 6 22.188796550470673 44.91188157284498 18.707930658443953 14.19139121824039 7 21.20450750467574 37.81347802795104 25.94540743919975 15.03660702817347 8 24.186327737068495 34.40778694302336 22.795354651144574 18.61053066876357 9 27.129300973367876 14.845191587745651 23.480502880630073 34.545004558256394 10-11 21.188373470007924 29.44101052314999 29.852350086622536 19.518265920219548 12-13 24.6535881779819 28.92581790157299 30.18239291230291 16.238201008142205 14-15 22.084673920194486 27.309594894752838 31.834173451837554 18.771557733215122 16-17 16.349729480797365 30.923618660342544 32.33103906316083 20.39561279569926 18-19 16.103176368495088 26.197599631580104 40.07224288144461 17.626981118480206 20-21 17.54725079182083 25.59860401439845 40.977315233974835 15.87682995980589 22-23 17.36742679377571 25.554744502680126 40.73185860946551 16.345970094078652 24-25 16.833700923570472 26.245316357347836 41.209632952793896 15.7113497662878 26-27 16.172255099451444 26.45652739183148 41.19019050692197 16.181027001795105 28-29 16.52535005427623 24.658795960532643 42.056795023817315 16.759058961373807 30-31 17.11307099442321 25.361238172817846 41.40578983645592 16.119900996303027 32-33 16.180400437341987 26.610975529525284 40.86547347909311 16.343150554039617 34-35 16.84565524544096 25.334977020748685 41.58148000789471 16.237887725915645 36-37 16.82772059480043 25.59073557446049 39.92111516465408 17.660428666085004 38-39 16.43650865755433 25.847350102286647 40.62706570468138 17.089075535477647 40-41 16.312292254723513 25.366775166744464 41.159332207605864 17.161600370926156 42-43 16.731150591633483 25.19243360766414 39.96854646445343 18.107869336248946 44-45 16.537146312311926 25.68732465408588 39.34891545957665 18.426613574025545 46-47 17.403781387553064 25.4360187346293 37.893920644120364 19.26627923369727 48-49 17.364450612623394 26.83732193821448 36.292022894665116 19.50620455449701 50-51 17.78907003847166 26.43814458827805 35.61275830524192 20.16002706800837 52-53 17.473942750805918 26.902328000225562 35.782469353166185 19.84125989580233 54-55 17.556795130257164 27.165326578852145 35.44974788178405 19.828130409106645 56-57 17.96109661310585 26.708249660871992 34.80111277846874 20.529540947553425 58-59 17.43354375851752 26.325130406178044 36.13574774040947 20.105578094894973 60-61 18.214100541822457 28.276271497068457 34.042866474206654 19.466761486902435 62-63 18.531038577155094 27.96362058513463 35.11998283162302 18.385358006087255 64-65 18.555103894985 26.74718234294352 35.57915677843308 19.1185569836384 66-67 18.502065322784027 27.40041886030523 34.65606932341686 19.44144649349388 68-69 18.64325803536044 27.429170249860196 34.987476444908104 18.940095269871254 70-71 19.005040900532286 26.784600973091177 35.040681226483365 19.16967689989317 72-73 18.42489046509843 27.185489543078507 34.326052937039165 20.0635670547839 74-75 17.879329951973837 27.907963947481367 34.23407194839615 19.978634152148647 76-77 17.5821989692821 27.331568476949826 35.593602656683224 19.492629897084853 78-79 17.250081062411006 27.003416369437826 35.233874845903095 20.512627722248066 80-81 17.459743107769423 28.467418546365913 33.965225563909776 20.107612781954888 82-83 18.07135685161428 26.741040449751484 34.47541263508444 20.712190063549798 84-85 17.402671044263645 26.692115626204178 34.24582003189213 21.659393297640044 86-87 17.575958845312766 27.700619701365348 33.99600859686828 20.727412856453604 88-89 17.824072986228927 26.66992498045795 34.505386399986214 21.00061563332691 90-91 17.930381957770507 26.75176896121209 34.402673062131775 20.915176018885628 92-93 18.097198463572507 27.594131248002707 33.63739183287278 20.671278455552006 94-95 17.608670792781023 26.619860175790645 34.0401301433488 21.73133888807954 96-97 18.237030167646772 27.077256620810005 33.64496229435778 21.040750917185438 98-99 17.708207686824938 27.16533644545049 33.57076280811135 21.555693059613233 100-101 17.410034526189147 27.164152917807616 34.0026568247185 21.423155731284737 102-103 18.389031087774484 26.898929487802054 33.15674215913256 21.555297265290903 104-105 17.931515217970208 27.532856091104186 32.98688889236987 21.54873979855574 106-107 18.169429293767877 26.540017105209568 33.63116030338251 21.659393297640044 108-109 18.116250798942264 27.15855400849699 32.32066096023461 22.404534232326142 110-111 18.021968037945932 27.651938807798466 32.071236344383145 22.254856809872457 112-113 17.674260498238482 27.49835909951768 33.09982157654899 21.727558825694857 114-115 17.8865354431847 28.006177925507753 32.254911482106884 21.852375149200657 116-117 17.52391126594215 28.121152502655068 32.13523767156118 22.219698559841603 118-119 17.469333153715656 27.436860993149985 32.36110045066284 22.732705402471517 120-121 17.15175884749554 28.02470547508541 32.048978620020954 22.77455705739809 122-123 17.24247042919376 28.30338527185091 31.967643248645405 22.486501050309922 124-125 16.97054698457223 28.71477038557672 31.628260477798587 22.686422152052465 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1221.0 1 889.5 2 999.0 3 1278.0 4 692.0 5 427.5 6 1016.0 7 2567.0 8 4253.5 9 4396.0 10 2665.5 11 1070.0 12 730.0 13 698.0 14 701.5 15 706.0 16 734.0 17 775.5 18 847.0 19 872.5 20 926.5 21 1029.0 22 1116.5 23 1283.5 24 1392.5 25 1513.0 26 1721.5 27 2017.0 28 2352.5 29 3053.0 30 4425.0 31 5508.5 32 7345.0 33 10307.0 34 12732.5 35 14082.0 36 16659.0 37 19068.5 38 18795.0 39 19120.0 40 18620.0 41 16314.0 42 13550.5 43 12597.5 44 12969.5 45 12236.0 46 10076.5 47 8199.5 48 7438.0 49 6166.0 50 5077.5 51 3796.0 52 2841.5 53 2534.0 54 2287.0 55 2013.0 56 1783.0 57 1549.0 58 1368.5 59 1245.0 60 1125.0 61 1040.5 62 910.0 63 564.0 64 287.0 65 279.0 66 242.5 67 167.0 68 106.0 69 59.5 70 44.5 71 42.5 72 46.0 73 72.0 74 67.0 75 37.5 76 24.5 77 18.5 78 18.5 79 7.5 80 1.5 81 0.0 82 0.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018170369140447555 2 0.001566411132797203 3 0.001566411132797203 4 0.08865887011632169 5 0.003132822265594406 6 0.02662898925755245 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001566411132797203 26-27 0.0 28-29 1.566411132797203E-4 30-31 0.006578926757748253 32-33 0.0 34-35 0.0 36-37 4.699233398391609E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0032894633788741264 46-47 0.0018796933593566436 48-49 0.0 50-51 0.001566411132797203 52-53 0.0 54-55 0.0017230522460769235 56-57 0.0 58-59 0.0018796933593566436 60-61 1.566411132797203E-4 62-63 0.0029761811523146857 64-65 0.0026628989257552454 66-67 4.699233398391609E-4 68-69 7.832055663986014E-4 70-71 0.003759386718713287 72-73 0.0032894633788741264 74-75 0.0 76-77 0.0018796933593566436 78-79 7.832055663986014E-4 80-81 3.132822265594406E-4 82-83 7.832055663986014E-4 84-85 0.0 86-87 0.004699233398391609 88-89 0.0054824389647902105 90-91 0.00516915673823077 92-93 0.005952362304629371 94-95 0.004542592285111889 96-97 0.00563908007806993 98-99 0.005795721191349651 100-101 0.007205491210867134 102-103 0.002506257812475525 104-105 0.0018796933593566436 106-107 0.0 108-109 0.009085184570223778 110-111 0.003132822265594406 112-113 0.00516915673823077 114-115 0.0 116-117 0.0 118-119 0.0014097700195174825 120-121 1.566411132797203E-4 122-123 0.0029761811523146857 124-125 0.04182317724568532 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 319201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.492407605239332 #Duplication Level Percentage of deduplicated Percentage of total 1 68.83368005547554 16.170688688318645 2 10.768389609004107 5.059507958934966 3 4.974129194004374 3.5056281152001407 4 2.784445511281805 2.616533156224448 5 1.9989865045073878 2.3480502880630074 6 1.4028911292473463 1.977437414043189 7 1.0348322398250387 1.7017490546708813 8 0.8708059956259667 1.636586351546518 9 0.6387688696858164 1.3505596786977485 >10 5.531551714941058 26.446345719468294 >50 0.7481196991518643 12.095826767460002 >100 0.39072918333600043 15.744938142424367 >500 0.013335467008054623 2.1669731611116507 >1k 0.0066677335040273115 3.0861432138370493 >5k 0.0026670934016109244 4.093032289999091 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7421 2.324867403297609 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5644 1.768164886701483 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4272 1.3383416718619303 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1702 0.5332063496041679 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1578 0.49435935351079724 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1210 0.37907149413692315 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1089 0.3411643447232308 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 917 0.28727980175500706 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 841 0.26347035253648954 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 746 0.23370854101334268 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 745 0.23339525878678324 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 687 0.21522488964633568 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 630 0.1973678027324476 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 609 0.19078887597469935 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 607 0.19016231152158045 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 600 0.18796933593566437 RNA PCR Primer, Index 17 (95% over 21bp) TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 535 0.16760599120930073 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 458 0.1434832597642238 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 447 0.14003715527206995 No Hit GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 424 0.13283166406120284 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 421 0.13189181738152447 No Hit GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC 417 0.13063868847528673 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 407 0.12750586620969231 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 398 0.12468632617065736 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 383 0.11998709277226575 No Hit ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA 382 0.11967381054570632 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 372 0.11654098828011189 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 371 0.11622770605355247 No Hit TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA 358 0.11215503710827973 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 349 0.10933549706924478 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 344 0.10776908593644757 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 342 0.10714252148332867 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 341 0.10682923925676925 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 340 0.1065159570302098 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 340 0.1065159570302098 RNA PCR Primer, Index 17 (95% over 23bp) GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 337 0.1055761103505315 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 335 0.1049495458974126 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 330 0.10338313476461541 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.132822265594406E-4 0.0 12-13 0.0 0.0 0.0 3.132822265594406E-4 0.0 14-15 0.0 0.0 0.0 3.132822265594406E-4 0.0 16-17 0.0 0.0 0.0 0.0015664111327972031 0.0 18-19 0.0 0.0 0.0 0.0017230522460769233 0.0 20-21 0.0 0.0 0.0 0.002819540039034965 0.0 22-23 0.0 0.0 0.0 0.008771902343664336 0.0 24-25 0.0 0.0 0.0 0.017230522460769233 0.0 26-27 0.0 0.0 0.0 0.02553250146459441 0.0 28-29 0.0 0.0 0.0 0.040883330566007 0.0 30-31 0.0 0.0 0.0 0.08239322558513287 0.0 32-33 0.0 0.0 0.0 0.13408479296744058 0.0 34-35 0.0 0.0 0.0 0.2050432172831539 0.0 36-37 0.0 0.0 0.0 0.30639001757513296 0.0 38-39 0.0 0.0 0.0 0.46788700536652456 0.0 40-41 0.0 0.0 0.0 0.6550731357357903 0.0 42-43 0.0 0.0 0.0 0.8228357680583707 0.0 44-45 0.0 0.0 0.0 0.9927913759668674 0.0 46-47 0.0 0.0 0.0 1.191099025378993 0.0 48-49 0.0 0.0 0.0 1.4129028417830771 0.0 50-51 0.0 0.0 0.0 1.6650950341634267 0.0 52-53 0.0 0.0 0.0 1.9103950175594688 0.0 54-55 0.0 0.0 0.0 2.134391809549469 0.0 56-57 0.0 0.0 0.0 2.3809449218517487 0.0 58-59 0.0 0.0 0.0 2.66023602682949 0.0 60-61 0.0 0.0 0.0 2.977120998994364 0.0 62-63 0.0 0.0 0.0 3.2925962011397205 0.0 64-65 0.0 0.0 0.0 3.59538347310942 0.0 66-67 0.0 0.0 0.0 3.924016528770273 0.0 68-69 0.0 0.0 0.0 4.278652009235561 0.0 70-71 0.0 0.0 0.0 4.694690806106497 0.0 72-73 0.0 0.0 0.0 5.111982731883672 0.0 74-75 0.0 0.0 0.0 5.536793431098273 0.0 76-77 0.0 0.0 0.0 5.95862794916056 0.0 78-79 0.0 0.0 0.0 6.42902121233956 0.0 80-81 0.0 0.0 0.0 6.960034586357812 0.0 82-83 0.0 0.0 0.0 7.53819693547326 0.0 84-85 0.0 0.0 0.0 8.096622504315462 0.0 86-87 0.0 0.0 0.0 8.631865188392268 0.0 88-89 0.0 0.0 0.0 9.182145419343925 0.0 90-91 0.0 0.0 0.0 9.824373983790778 0.0 92-93 0.0 0.0 0.0 10.52894571132296 0.0 94-95 0.0 0.0 0.0 11.228348282116912 0.0 96-97 0.0 0.0 0.0 11.947017709844268 0.0 98-99 0.0 0.0 0.0 12.644697228392141 0.0 100-101 0.0 0.0 0.0 13.430722334829778 0.0 102-103 0.0 0.0 0.0 14.304309823590778 0.0 104-105 0.0 0.0 0.0 15.192308294773513 0.0 106-107 0.0 0.0 0.0 16.053521135585417 0.0 108-109 0.0 0.0 0.0 16.8685248479798 0.0 110-111 0.0 0.0 0.0 17.755896754709415 0.0 112-113 0.0 0.0 0.0 18.704358695618122 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1355 0.0 97.0926 1 GTATCAA 3440 0.0 71.47016 1 CTACTCG 35 9.148156E-5 67.99123 9 GTACAGC 40 1.7656818E-4 59.52962 3 TCAACGC 4630 0.0 53.358086 4 ATCAACG 4695 0.0 52.61937 3 CAACGCA 4735 0.0 52.26781 5 ACATATA 80 3.8200596E-8 52.088417 2 AACGCAG 4760 0.0 51.86831 6 TATCAAC 4800 0.0 51.3443 2 ATTAACG 25 5.330084E-4 47.601315 66-67 ACGCAGA 5370 0.0 45.976376 7 CGCAGAG 5385 0.0 45.73783 8 GTATGGT 145 1.8189894E-12 45.160404 1 GTACTAC 55 8.524552E-4 43.267147 6 GCAGAGT 5700 0.0 42.897095 9 GGATATG 180 0.0 39.686417 1 CAGAGTC 60 0.0013038977 39.686413 4 ACCGCGT 30 0.0013068904 39.673977 42-43 AAACCGC 30 0.0013068904 39.673977 40-41 >>END_MODULE