FastQCFastQC Report
Fri 27 May 2016
SRR940125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385606
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA14470.10443084109046871No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14370.10370913520871011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.065.710661
GTATCAA33350.059.607271
TCAACGC40150.048.02954
ATCAACG40550.047.8492743
CAACGCA41900.045.7377475
TATCAAC43500.044.7411422
AACGCAG43600.043.809986
GTACATG89450.037.1280751
ACGCAGA52800.035.60657
TACATGG92200.034.858722
CGCAGAG54000.034.599898
ACATGGG96500.031.763513
CATGGGG43200.030.0345654
GCAGAGT59700.029.7016649
GTAAGGT8100.028.6568324
TAAGGTG9100.028.1228925
AACGCGG850.007207829528.002426
GGTAAGG8600.027.6828063
AGGTAAG7550.027.5911452
CATGGGA58100.026.1223724
Produced by FastQC (version 0.11.5)