FastQCFastQC Report
Fri 27 May 2016
SRR940125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385606
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43880.31668454091567155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35770.2581541939050495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.15300164693282217No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA14460.10435867050229287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.079.200031
GTATCAA56800.061.444971
ATCAACG76500.045.6136473
TCAACGC76250.045.607014
TATCAAC76950.045.504962
CAACGCA78900.044.1235965
AACGCAG81550.042.761216
GTACATG90450.037.4662931
ACGCAGA94500.036.641527
CGCAGAG95100.036.3477868
TACATGG93850.035.6608772
GTGGTAT11800.034.8260421
ACATGGG96650.033.3930443
TGGTATC12000.032.753122
GCAGAGT104350.032.384589
CATGGGG44050.029.6044374
CATGGGA56300.029.5089954
ATGGGAG25350.027.9357975
GAGTACT57050.027.37511812-13
AGTACTT57750.026.47667712-13
Produced by FastQC (version 0.11.5)