FastQCFastQC Report
Fri 27 May 2016
SRR940124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911543
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15000.16455614271625146No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA9660.10597415590926594No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG9390.10301214534037342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.075.707921
GTATCAA28150.056.873241
ATCAACG34950.043.9345973
TCAACGC35100.043.577284
CAACGCA36450.042.126595
TATCAAC38100.041.70812
GTACATG82750.040.2767261
AACGCAG37800.040.1387026
TACATGG85650.038.7045442
ACATGGG85650.036.7588923
CATGGGA58050.033.4233134
CATGGGG28350.033.1694374
ACGCAGA46250.032.8016557
CGCAGAG47650.031.5899378
ATGGGAG21200.030.6002355
GTGGTAT7150.029.1337321
ATGGGAT23950.028.5776445
TGGTATC6600.027.954512
GCAGAGT51700.027.6192479
GAGTACA59350.026.5741441
Produced by FastQC (version 0.11.5)