##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940124_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 911543 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.905700553895976 33.0 31.0 34.0 27.0 34.0 2 31.021991282912598 33.0 31.0 34.0 27.0 34.0 3 31.122486816310367 34.0 31.0 34.0 27.0 34.0 4 34.27987599049085 37.0 35.0 37.0 30.0 37.0 5 34.4015136971048 37.0 35.0 37.0 31.0 37.0 6 34.376175342249354 37.0 35.0 37.0 30.0 37.0 7 34.28034881514092 37.0 35.0 37.0 30.0 37.0 8 34.154498471273435 37.0 35.0 37.0 30.0 37.0 9 35.58507058910002 39.0 37.0 39.0 29.0 39.0 10-11 35.608847306161096 39.0 37.0 39.0 29.5 39.0 12-13 35.51524118993838 39.0 37.0 39.0 28.5 39.0 14-15 36.85150069716953 41.0 38.0 41.0 28.5 41.0 16-17 36.89839755228223 40.0 38.0 41.0 29.0 41.0 18-19 36.946062884581416 40.5 38.0 41.0 29.0 41.0 20-21 37.02553527370623 40.0 38.0 41.0 29.0 41.0 22-23 37.069552944841874 40.0 38.0 41.0 30.0 41.0 24-25 37.132695330884005 40.0 38.0 41.0 30.0 41.0 26-27 37.12998728529537 40.0 38.0 41.0 30.0 41.0 28-29 37.06751903091791 40.0 38.0 41.0 30.0 41.0 30-31 36.99230974293039 40.0 37.0 41.0 30.0 41.0 32-33 36.9025438185582 40.0 37.0 41.0 30.0 41.0 34-35 36.81241312806966 40.0 37.0 41.0 30.0 41.0 36-37 36.67348605606098 40.0 37.0 41.0 30.0 41.0 38-39 36.513241832804376 40.0 36.0 41.0 29.0 41.0 40-41 36.38953181583315 40.0 36.0 41.0 29.0 41.0 42-43 36.353658028200535 40.0 36.0 41.0 28.5 41.0 44-45 36.42671656740275 40.0 36.0 41.0 29.0 41.0 46-47 36.33320095705852 40.0 36.0 41.0 29.0 41.0 48-49 36.21337885321921 40.0 35.5 41.0 29.0 41.0 50-51 36.080081795373346 40.0 35.0 41.0 29.0 41.0 52-53 35.90239516950928 40.0 35.0 41.0 28.0 41.0 54-55 35.72144594385564 39.5 35.0 41.0 28.0 41.0 56-57 35.514308156609175 39.0 35.0 41.0 28.0 41.0 58-59 35.26939211863839 39.0 35.0 41.0 27.5 41.0 60-61 35.020119182528965 38.5 34.0 41.0 27.0 41.0 62-63 34.73909075051863 38.0 34.0 40.5 26.0 41.0 64-65 34.43557352752421 37.0 34.0 40.0 26.0 41.0 66-67 34.10200725582885 37.0 34.0 40.0 26.0 41.0 68-69 33.76814204047423 36.0 33.5 39.5 25.5 41.0 70-71 33.659217941446535 36.0 34.0 39.0 26.0 41.0 72-73 33.42885634577853 35.5 34.0 39.0 26.0 41.0 74-75 33.09528842852175 35.0 34.0 38.5 26.0 40.5 76-77 32.73906716413817 35.0 33.5 37.0 25.0 39.5 78-79 32.41151377389767 35.0 33.0 37.0 25.0 39.0 80-81 32.09907760796803 35.0 33.0 36.0 24.0 39.0 82-83 31.81572893434539 35.0 33.0 36.0 23.5 37.0 84-85 31.556085121601505 35.0 33.0 36.0 22.5 37.0 86-87 31.349742140524363 35.0 33.0 35.0 21.0 37.0 88-89 31.16180202140766 35.0 33.0 35.0 20.0 36.0 90-91 31.01710396547393 35.0 33.0 35.0 19.5 36.0 92-93 30.873793117823297 35.0 33.0 35.0 18.5 36.0 94-95 30.76759845668279 35.0 33.0 35.0 18.0 36.0 96-97 30.638151464056 35.0 32.0 35.0 17.0 35.0 98-99 30.55808557577646 35.0 32.0 35.0 14.0 35.0 100-101 30.494648085718392 35.0 32.0 35.0 10.5 35.0 102-103 29.699458500586367 34.0 30.5 35.0 7.5 35.0 104-105 30.295728780759656 35.0 32.0 35.0 7.0 35.0 106-107 30.368078082986763 35.0 32.0 35.0 7.0 35.0 108-109 30.337256717456007 35.0 32.0 35.0 2.0 35.0 110-111 30.25016263632105 35.0 32.0 35.0 2.0 35.0 112-113 30.154588428631453 35.0 32.0 35.0 2.0 35.0 114-115 30.036313701054148 35.0 32.0 35.0 2.0 35.0 116-117 29.95951315516657 35.0 32.0 35.0 2.0 35.0 118-119 29.841408468936738 35.0 32.0 35.0 2.0 35.0 120-121 29.696656657996385 35.0 32.0 35.0 2.0 35.0 122-123 29.46403954613222 35.0 31.0 35.0 2.0 35.0 124-125 27.437584403588204 33.0 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5535293563407109 1101 2 0.5814089906015454 1101 3 0.49929877446906445 1101 4 0.5494812026521245 1101 5 0.5167067726843513 1101 6 0.6512759770130856 1101 7 0.726591043324909 1101 8 0.6533802465783083 1101 9 0.8772543415193397 1101 10-11 0.7021499713707016 1101 12-13 0.7507043680367858 1101 14-15 0.8592542677940784 1101 16-17 0.8816699344013728 1101 18-19 0.7777949523248466 1101 20-21 0.8008581294679686 1101 22-23 0.710139181807115 1101 24-25 0.71546590772369 1101 26-27 0.7331237808610993 1101 28-29 0.72022613328825 1101 30-31 0.6915794774083608 1101 32-33 0.6885476450796091 1101 34-35 0.8372897487554241 1101 36-37 0.8429075280541198 1101 38-39 0.8754686447458724 1101 40-41 0.9023892731217487 1101 42-43 0.8409052864087982 1101 44-45 0.76488759288182 1101 46-47 0.7091653091721497 1101 48-49 0.7099793029405816 1101 50-51 0.6793253230655694 1101 52-53 0.6538344307877821 1101 54-55 0.6303938232667647 1101 56-57 0.5789562435341509 1101 58-59 0.6377448239423416 1101 60-61 0.6148190566165042 1101 62-63 0.5788539312095367 1101 64-65 0.5545777693877483 1101 66-67 0.5779170672301106 1101 68-69 0.5430558580896445 1101 70-71 0.6513197457836242 1101 72-73 0.5516612817339066 1101 74-75 0.589225847672985 1101 76-77 0.5180168021338503 1101 78-79 0.5232532408524264 1101 80-81 0.6579950426729155 1101 82-83 0.5248787510801378 1101 84-85 0.4236724784532093 1101 86-87 0.5076378069442917 1101 88-89 0.4686193923300692 1101 90-91 0.4163602661165342 1101 92-93 0.42229519335694476 1101 94-95 0.4117614915070327 1101 96-97 0.3737510677072784 1101 98-99 0.4273468280185462 1101 100-101 0.5147857975904486 1101 102-103 0.5337726807431764 1101 104-105 0.48904344881875517 1101 106-107 0.451899907668075 1101 108-109 0.4631755436063685 1101 110-111 0.4569849240055426 1101 112-113 0.41124755657808976 1101 114-115 0.3859113976166988 1101 116-117 0.460854239697742 1101 118-119 0.491724998827511 1101 120-121 0.39060705091634773 1101 122-123 0.4252187407328769 1101 124-125 0.10378036872076635 1102 1 0.266055898685682 1102 2 0.4539094359832987 1102 3 0.4186726399493317 1102 4 0.5459470382605147 1102 5 0.22415114811468584 1102 6 0.5792613588617357 1102 7 0.54082111068589 1102 8 0.5703466431269604 1102 9 0.5423393010979396 1102 10-11 0.5848183617851248 1102 12-13 0.5918878556888814 1102 14-15 0.6698725298904051 1102 16-17 0.5265435904318423 1102 18-19 0.4801607720439449 1102 20-21 0.31051268175097135 1102 22-23 0.43712678815705175 1102 24-25 0.4492979482462047 1102 26-27 0.3659324063407894 1102 28-29 0.612050729916831 1102 30-31 0.635158637394305 1102 32-33 0.30821690699778514 1102 34-35 0.6388053708213164 1102 36-37 0.4444214648917608 1102 38-39 0.4051292638505828 1102 40-41 0.5940641251827898 1102 42-43 0.4835412000673287 1102 44-45 0.4344648894868115 1102 46-47 0.5267513882238006 1102 48-49 0.5550592790584901 1102 50-51 0.44176411316797726 1102 52-53 0.48402300782919383 1102 54-55 0.3284544866689103 1102 56-57 0.3306348512467139 1102 58-59 0.24703127422569793 1102 60-61 0.41791036709813767 1102 62-63 0.5437596547261734 1102 64-65 0.4401218814409802 1102 66-67 0.4556320903177422 1102 68-69 0.32508542662306894 1102 70-71 0.5244828469888034 1102 72-73 0.3561754063541329 1102 74-75 0.36957379915767063 1102 76-77 0.42904698216437254 1102 78-79 0.3924163324277288 1102 80-81 0.4413384873757522 1102 82-83 0.3676638074389871 1102 84-85 0.178686634370532 1102 86-87 0.3014717975166441 1102 88-89 0.5049240238187949 1102 90-91 0.47320301506085727 1102 92-93 0.473471964170713 1102 94-95 0.5862024772211107 1102 96-97 0.503640637107388 1102 98-99 0.4969786537948302 1102 100-101 0.41714969133439794 1102 102-103 0.36659021385791135 1102 104-105 0.5232981205122158 1102 106-107 0.4478348963770493 1102 108-109 0.45067114997556956 1102 110-111 0.47763378503199405 1102 112-113 0.5079218789959619 1102 114-115 0.42652660222748295 1102 116-117 0.4560471261085439 1102 118-119 0.5096323546080868 1102 120-121 0.57210492993622 1102 122-123 0.4946682007375962 1102 124-125 0.3177658372382979 1103 1 0.024873641227852517 1103 2 0.044457066742438656 1103 3 0.06763871924873399 1103 4 -0.02041629999165906 1103 5 -0.032154128545904825 1103 6 -0.03517398733773547 1103 7 -0.18123072037609944 1103 8 -0.07674593714174449 1103 9 -0.03219316806286798 1103 10-11 -0.05545897394010524 1103 12-13 -0.1950263443246243 1103 14-15 -0.03744327887284982 1103 16-17 -0.36399294882724575 1103 18-19 -0.18391747607773112 1103 20-21 0.03280804357498823 1103 22-23 -0.19962055711652482 1103 24-25 0.021772461908035723 1103 26-27 0.03096066555396959 1103 28-29 -0.16745145462643052 1103 30-31 -0.07358218800619909 1103 32-33 -0.01328589481157394 1103 34-35 -0.017718601285977797 1103 36-37 -0.1609092064815556 1103 38-39 -0.2252923095992614 1103 40-41 -0.0766183878617781 1103 42-43 -0.10071321000953759 1103 44-45 -0.0853852788146483 1103 46-47 -0.21486247325783125 1103 48-49 -0.18520913524280047 1103 50-51 -0.1410351403258403 1103 52-53 -0.1923788613296864 1103 54-55 -0.11437244735113694 1103 56-57 -0.18595779171307925 1103 58-59 -0.2741245565276529 1103 60-61 -0.37447363027848013 1103 62-63 -0.25614003953421616 1103 64-65 -0.1613605216530658 1103 66-67 -0.30296743901686085 1103 68-69 -0.24399894593560134 1103 70-71 -0.34316300623689244 1103 72-73 -0.5021318860660315 1103 74-75 -0.4336895130535865 1103 76-77 -0.6409982518662716 1103 78-79 -0.4466375626177417 1103 80-81 -0.5621577463366165 1103 82-83 -0.5202240333835206 1103 84-85 -0.45946402677931886 1103 86-87 -0.42250416768498056 1103 88-89 -0.371687860482389 1103 90-91 -0.28573167047100156 1103 92-93 -0.24400341752155796 1103 94-95 -0.3476065850166741 1103 96-97 -0.46405416339028704 1103 98-99 -0.5366768152791721 1103 100-101 -0.5699455894734484 1103 102-103 -0.41555584673061574 1103 104-105 -0.30499943067746926 1103 106-107 -0.4848987725947218 1103 108-109 -0.4996039674496586 1103 110-111 -0.2660006584158232 1103 112-113 -0.3847075780992206 1103 114-115 -0.29262576604889645 1103 116-117 -0.36742321136240363 1103 118-119 -0.29372499423405785 1103 120-121 -0.19050080099363598 1103 122-123 -0.27034269786680554 1103 124-125 0.11550751137967197 1104 1 -0.06063169280176339 1104 2 -0.06873253376485167 1104 3 -0.02179952910070071 1104 4 -0.20637064368221303 1104 5 -0.08933175546223993 1104 6 -0.15731813669057004 1104 7 -0.17070020823465626 1104 8 -0.1354062750879521 1104 9 -0.06543760090258388 1104 10-11 0.06444129276137289 1104 12-13 -0.07532773564843609 1104 14-15 -0.13617877814548507 1104 16-17 -0.1023364155734896 1104 18-19 -0.02111184177582004 1104 20-21 -0.023726142705733366 1104 22-23 -0.05043091930831878 1104 24-25 -0.13033014356027905 1104 26-27 -0.18004589940555604 1104 28-29 0.006333468347193616 1104 30-31 -0.012478640689998599 1104 32-33 -0.0056753442501999984 1104 34-35 -0.0456896184816884 1104 36-37 0.023502977859521934 1104 38-39 0.0212620191709334 1104 40-41 0.10075091908738187 1104 42-43 0.09256843048432728 1104 44-45 0.07693078109060991 1104 46-47 -0.13281796395126122 1104 48-49 -0.045352883610881634 1104 50-51 -0.06886954295183045 1104 52-53 -0.04704753602848655 1104 54-55 -0.04841162262809462 1104 56-57 -0.07912216748241008 1104 58-59 0.03254834249879224 1104 60-61 0.09774418836450849 1104 62-63 0.010561441311764952 1104 64-65 -0.053201880898093634 1104 66-67 0.002425572658317776 1104 68-69 0.11223636683250504 1104 70-71 -0.024644738117977738 1104 72-73 0.10513502989501688 1104 74-75 0.004747672220020149 1104 76-77 -0.3399601210146983 1104 78-79 -0.20213711500686316 1104 80-81 -0.14664663066650618 1104 82-83 -0.04052971431156749 1104 84-85 0.029252449031041294 1104 86-87 -0.009953811461326012 1104 88-89 -0.15185613419643929 1104 90-91 -0.09136014411791038 1104 92-93 0.013848423278172817 1104 94-95 -0.047756254903649875 1104 96-97 0.15278276637210908 1104 98-99 0.18737341039131294 1104 100-101 0.16240641918036403 1104 102-103 0.15617612760636135 1104 104-105 0.06197854313729323 1104 106-107 -0.04558035340713573 1104 108-109 -0.07034163950008931 1104 110-111 -0.025202325766340294 1104 112-113 -0.04480773103293956 1104 114-115 0.09591912559196913 1104 116-117 0.14480449202078916 1104 118-119 0.11380605877053185 1104 120-121 0.12739384259620223 1104 122-123 0.23945928094628144 1104 124-125 0.3371576568532042 1105 1 0.20419917260999654 1105 2 0.30306986858832374 1105 3 0.19787736441440273 1105 4 0.4424969354779691 1105 5 0.43572425087603506 1105 6 0.4286407783346107 1105 7 0.513161656457676 1105 8 0.44679213305710164 1105 9 0.5776244431921569 1105 10-11 0.5803026406472469 1105 12-13 0.5927823464031263 1105 14-15 0.5903440933860011 1105 16-17 0.4846544048510637 1105 18-19 0.5273948266211264 1105 20-21 0.6210775619086419 1105 22-23 0.7554445098459368 1105 24-25 0.6078718139295418 1105 26-27 0.671010252640265 1105 28-29 0.5864407455284777 1105 30-31 0.46144995350537243 1105 32-33 0.5745261305833793 1105 34-35 0.5884057197874881 1105 36-37 0.4181174334141673 1105 38-39 0.626531168329926 1105 40-41 0.7417539903292649 1105 42-43 0.7258492863340322 1105 44-45 0.7822813784915468 1105 46-47 0.6866683144958685 1105 48-49 0.6311510345561189 1105 50-51 0.7136283625021917 1105 52-53 0.6411434364783233 1105 54-55 0.5543933045636678 1105 56-57 0.534851584669056 1105 58-59 0.5715777594864733 1105 60-61 0.6274110417189718 1105 62-63 0.6102424783852314 1105 64-65 0.43091030480617576 1105 66-67 0.2836920785913506 1105 68-69 0.2829913823594623 1105 70-71 0.3798835223160495 1105 72-73 0.3494777636418007 1105 74-75 0.28105983697882664 1105 76-77 0.2379623967206399 1105 78-79 0.3142088812229531 1105 80-81 0.3286997662590707 1105 82-83 0.30640223115737797 1105 84-85 0.37694480575954614 1105 86-87 0.35800043181563623 1105 88-89 0.3441417462140919 1105 90-91 0.3619311958451128 1105 92-93 0.3581895064316498 1105 94-95 0.3212486763861335 1105 96-97 0.42491750714927434 1105 98-99 0.3950482139137925 1105 100-101 0.38637144026242254 1105 102-103 0.3340150342533583 1105 104-105 0.34569447261484143 1105 106-107 0.37025703241733865 1105 108-109 0.3168878339492629 1105 110-111 0.2934640631453149 1105 112-113 0.351665918667905 1105 114-115 0.25398790866075416 1105 116-117 0.36746782107396214 1105 118-119 0.4107500706456939 1105 120-121 0.6124320680604178 1105 122-123 0.5158445840635117 1105 124-125 0.5647053191577527 1106 1 -0.1217736919225949 1106 2 -0.06254022917792668 1106 3 -0.014277258334839615 1106 4 0.18576977203875344 1106 5 0.08643347971525372 1106 6 0.10502666320895315 1106 7 -0.08451088653983163 1106 8 -0.007145278757135998 1106 9 0.04304098955753943 1106 10-11 0.09537674342477942 1106 12-13 0.08012355574199859 1106 14-15 0.06257827538509986 1106 16-17 0.2671598120772458 1106 18-19 0.1359820027011196 1106 20-21 0.16151145698566438 1106 22-23 0.16064906705560134 1106 24-25 0.11321150254760681 1106 26-27 0.12870922533764428 1106 28-29 -0.029739341133470987 1106 30-31 0.06233700312128576 1106 32-33 0.05030406482317318 1106 34-35 0.056898320765995436 1106 36-37 0.17448974323824018 1106 38-39 0.07389548677178226 1106 40-41 0.23318488054751896 1106 42-43 0.23665501509530174 1106 44-45 0.26491550105759387 1106 46-47 0.25136592349727493 1106 48-49 0.2554333775917854 1106 50-51 0.31442592484639675 1106 52-53 0.34392046852883595 1106 54-55 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98-99 -0.254288692565968 2215 100-101 -0.1959168425184714 2215 102-103 -0.23887004454305227 2215 104-105 -0.30518116151202435 2215 106-107 -0.41596455478946837 2215 108-109 -0.4143324145123657 2215 110-111 -0.44088055592836994 2215 112-113 -0.4159987647603245 2215 114-115 -0.5187360468218252 2215 116-117 -0.40169293971241515 2215 118-119 -0.48328481255174793 2215 120-121 -0.658234721818193 2215 122-123 -0.7156028956598277 2215 124-125 -0.7039287105600209 2216 1 -0.7099790823599967 2216 2 -0.7725477948090287 2216 3 -0.7928838664500972 2216 4 -0.7097173638964023 2216 5 -0.6915740580057488 2216 6 -0.7902665971992704 2216 7 -0.8352751747674532 2216 8 -0.8470863286354771 2216 9 -0.8184496490953563 2216 10-11 -1.0193522146014118 2216 12-13 -1.04252987754937 2216 14-15 -1.2700475867130834 2216 16-17 -1.265154353993836 2216 18-19 -1.2039682674786292 2216 20-21 -1.2971531842050652 2216 22-23 -1.4535433691075141 2216 24-25 -1.4035480150462192 2216 26-27 -1.4432654442516153 2216 28-29 -1.467562113982126 2216 30-31 -1.569205548320518 2216 32-33 -1.6150712612515434 2216 34-35 -1.6549600334749925 2216 36-37 -1.3722048534657745 2216 38-39 -1.501161006264141 2216 40-41 -1.5197728426261463 2216 42-43 -1.4143760139601156 2216 44-45 -1.5441786566603923 2216 46-47 -1.5138002263341193 2216 48-49 -1.4976202865559998 2216 50-51 -1.4749103729384387 2216 52-53 -1.4728340152788135 2216 54-55 -1.5056154378891407 2216 56-57 -1.7367108555680772 2216 58-59 -1.605211028317811 2216 60-61 -1.5999633082887712 2216 62-63 -1.500984743427189 2216 64-65 -1.6494200870560576 2216 66-67 -1.4326062211837112 2216 68-69 -1.2805849183080582 2216 70-71 -1.3947501598047225 2216 72-73 -1.313414166892123 2216 74-75 -1.2644123010062174 2216 76-77 -1.4125421156599458 2216 78-79 -1.507274330535651 2216 80-81 -1.2926473000105503 2216 82-83 -1.2918684023972418 2216 84-85 -1.3867448735927859 2216 86-87 -1.526269072505503 2216 88-89 -1.359561957489614 2216 90-91 -1.3756221923106828 2216 92-93 -1.4249065968021455 2216 94-95 -1.3055373794016454 2216 96-97 -1.275555587041545 2216 98-99 -1.3525362281629043 2216 100-101 -1.531870689253374 2216 102-103 -1.524965103986677 2216 104-105 -1.5047048571304416 2216 106-107 -1.5819759357364553 2216 108-109 -1.6187435805978154 2216 110-111 -1.6241815761010123 2216 112-113 -1.4626457497745733 2216 114-115 -1.4962830163089684 2216 116-117 -1.4277356686217821 2216 118-119 -1.6323452129831502 2216 120-121 -1.7013377504426153 2216 122-123 -1.8863355651045879 2216 124-125 -2.0720207233342087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19149.0 3 3928.0 4 2680.0 5 2097.0 6 2153.0 7 2432.0 8 2422.0 9 2775.0 10 3296.0 11 3754.0 12 4160.0 13 4185.0 14 4273.0 15 4009.0 16 3787.0 17 3614.0 18 3296.0 19 3116.0 20 2956.0 21 2873.0 22 3125.0 23 3878.0 24 5898.0 25 8627.0 26 12206.0 27 10561.0 28 10423.0 29 13270.0 30 14612.0 31 16394.0 32 20637.0 33 27447.0 34 39842.0 35 64641.0 36 137814.0 37 290666.0 38 149651.0 39 896.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.24562714445757 19.08420483211373 13.245793927982435 22.42437409544626 2 15.017960944545994 21.258538854668856 40.01757692923805 23.7059232715471 3 18.586644781739544 28.011902123466975 28.693246027688236 24.708207067105242 4 12.022136771270922 17.18968794307562 37.36134339430493 33.42683189134853 5 12.73092999700415 41.08756986198246 32.6457513270073 13.535748814006086 6 30.40432591864323 36.999530363946 18.535371934198285 14.060771783212486 7 28.46969271413901 34.55770057841011 20.52006442382804 16.452542283622833 8 27.265616429339364 35.35544243807183 19.7053338306602 17.673607301928605 9 28.307358096209867 17.17766842760671 19.023089709198032 35.491883766985396 10-11 24.683558190407023 28.635104032507552 26.482433063972916 20.198904713112505 12-13 25.568054344958174 25.983628274166893 26.86195057544592 21.586366805429012 14-15 22.793646892433756 26.65651009193035 24.98698425986933 25.56285875576656 16-17 22.206662311354364 30.26925593822697 25.4505471841041 22.073534566314567 18-19 22.61155196871904 28.716177298265134 27.249553199682712 21.422717533333113 20-21 24.13268020822477 27.541236293423587 26.707185290423084 21.61889820792856 22-23 23.650386873189188 27.90983161444546 26.47527971924405 21.964501793121304 24-25 22.930545315709093 27.948307792230594 26.846628311127496 22.27451858093282 26-27 22.446043323315504 28.175513676557788 26.661101003081043 22.717341997045665 28-29 22.49068154328454 28.3606373028999 26.642969399257815 22.505711754557748 30-31 22.614356426954064 27.860281985571607 26.820114619718805 22.705246967755524 32-33 22.315276745480826 28.17233614819 26.650667968503594 22.861719137825585 34-35 22.92279295809027 28.181461643313394 26.511544991045398 22.384200407550935 36-37 23.082817879218076 27.76013884896664 26.364518033031487 22.7925252387838 38-39 22.70705709922592 27.853260869565215 26.49127406926331 22.948407961945552 40-41 22.92411712267709 27.698717684975655 26.635452238908318 22.741712953438935 42-43 22.535941387890517 27.717381764243804 26.662673179123274 23.084003668742405 44-45 22.47352548497757 27.793934859473612 26.917119107617133 22.81542054793168 46-47 22.969537717166506 27.95568623793824 26.309527074841878 22.765248970053374 48-49 22.55349283178501 27.86931556382498 26.44861791900158 23.128573685388425 50-51 22.83650127540525 27.85380838750377 26.544940892509395 22.764749444581582 52-53 23.009199071864664 27.75316646644835 26.557287124042105 22.680347337644886 54-55 22.41105232035756 27.987787418583704 26.79636957649813 22.804790684560608 56-57 22.604805118789468 28.08169919761088 26.504184744238813 22.80931093936084 58-59 22.65764166889311 27.59705045400208 26.58294514614692 23.162362730957888 60-61 22.41325592518968 27.87422712532308 26.923410258773146 22.789106690714096 62-63 22.42907499909489 27.957763708034417 26.875196792946944 22.737964499923752 64-65 22.13424404008002 28.01108909066275 26.70213098576947 23.152535883487754 66-67 22.157898172359562 28.12133000486544 26.552485078667143 23.16828674410785 68-69 22.495428081834866 27.740764835010527 26.853368409389354 22.910438673765256 70-71 22.23048090589288 27.826266307624202 26.77338470661224 23.169868079870675 72-73 22.263812309251797 28.016920778197825 26.551382163411013 23.16788474913937 74-75 22.198555338817368 28.258935330347757 26.660405883088643 22.88210344774623 76-77 22.380778136985068 27.78821630630428 26.721420025114647 23.109585531596007 78-79 22.37894920455038 27.814050794901092 26.777220898743153 23.029779101805374 80-81 22.430178246714956 28.137199779488597 26.643427032393785 22.78919494140266 82-83 22.47384351416789 27.99805382800184 26.649556296919584 22.878546360910683 84-85 22.222429441068606 27.947941018690287 26.81190026142486 23.017729278816248 86-87 22.27965319808805 28.121211722282013 26.562786263519868 23.036348816110074 88-89 22.315858416807778 28.01965756689612 26.644242251700895 23.020241764595205 90-91 22.231846105152407 28.086391140810452 26.64800157541562 23.03376117862152 92-93 22.337389609919388 27.803159221747876 26.85870618272226 23.000744985610478 94-95 22.045696143791353 27.707579346229416 27.07831665648247 23.168407853496763 96-97 22.227134549371996 27.672376058512032 26.93735539565504 23.16313399646093 98-99 21.955586370926866 27.797738288269507 26.790412080015624 23.456263260788003 100-101 21.795241694577218 27.82430450346281 27.029937150523892 23.35051665143608 102-103 21.87462460609352 27.636506251765553 27.02556381079324 23.46330533134769 104-105 21.56412065252332 27.869600659149928 26.99645739826677 23.56982129005998 106-107 21.77527554926098 27.93724486941373 26.757322474090632 23.530157107234658 108-109 21.90878543305143 27.931649960561376 26.55420534193121 23.605359264455984 110-111 21.756697457050706 28.19344186760866 26.769368321741226 23.280492353599403 112-113 21.68208560183217 28.30378913589051 26.92582180227376 23.088303460003566 114-115 21.546286118004314 28.425135432231226 26.65299728148182 23.37558116828264 116-117 21.41729147822672 28.420922141229994 26.75736650498085 23.40441987556244 118-119 21.259665429140664 28.502648393410396 26.631219445834102 23.606466731614837 120-121 21.38588400885767 28.49297305124974 26.793461298163024 23.327681641729562 122-123 21.333606862210562 28.456968020159223 26.8295077686955 23.37991734893472 124-125 21.3763559382975 28.43900974304897 26.52882540515527 23.65580891349826 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1081.0 1 978.5 2 906.5 3 799.5 4 526.0 5 366.0 6 337.5 7 321.5 8 347.5 9 365.5 10 299.5 11 267.5 12 297.0 13 315.0 14 338.0 15 359.5 16 407.0 17 474.0 18 554.0 19 662.0 20 807.5 21 1113.5 22 1580.0 23 2075.0 24 2634.5 25 3459.5 26 4540.5 27 6017.0 28 7488.0 29 9128.0 30 11244.0 31 13445.0 32 16439.0 33 18295.0 34 19349.0 35 20410.0 36 21895.5 37 24291.0 38 25449.0 39 25857.0 40 26123.0 41 26832.0 42 27521.5 43 28988.5 44 31010.0 45 32713.0 46 33841.5 47 34028.0 48 33776.5 49 32748.0 50 31896.0 51 30947.5 52 30248.5 53 30515.0 54 30624.5 55 29252.5 56 26264.0 57 23968.5 58 22952.0 59 20727.0 60 17091.5 61 14266.0 62 12317.5 63 9414.5 64 6774.0 65 5480.5 66 4194.5 67 3145.0 68 2390.0 69 1683.5 70 1279.0 71 953.5 72 688.5 73 577.0 74 442.0 75 307.5 76 207.5 77 120.0 78 72.0 79 47.5 80 33.0 81 22.5 82 14.5 83 7.5 84 8.0 85 10.5 86 9.0 87 4.0 88 0.5 89 0.5 90 2.0 91 4.0 92 3.0 93 1.5 94 2.0 95 1.5 96 1.5 97 1.0 98 1.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01996614531623851 2 0.013274195512444282 3 0.011957746370714272 4 0.03280152444810612 5 0.030936554830655275 6 0.02183111493368936 7 0.008666623516389242 8 3.2911228543250294E-4 9 0.010751001324128429 10-11 1.0970409514416763E-4 12-13 0.003565383092185448 14-15 0.016071649938620557 16-17 0.0013713011893020956 18-19 0.006801653898938394 20-21 0.0030717146640366942 22-23 4.388163805766705E-4 24-25 0.0077889907552359024 26-27 1.6455614271625147E-4 28-29 0.004991536329059627 30-31 7.679286660091736E-4 32-33 0.0011518929990137602 34-35 5.4852047572083814E-5 36-37 0.005375500662064214 38-39 7.679286660091736E-4 40-41 0.011573782037709686 42-43 0.006472541613505891 44-45 6.582245708650059E-4 46-47 0.009653960372686753 48-49 0.002084377807739185 50-51 0.007459878469803399 52-53 0.00416875561547837 54-55 0.0026328982834600235 56-57 0.008502067373672992 58-59 0.016291058128908895 60-61 0.001206745046585844 62-63 0.005704612947496718 64-65 0.0018101175698787661 66-67 0.0013713011893020956 68-69 0.0 70-71 0.00488183223391546 72-73 1.0970409514416763E-4 74-75 0.005375500662064214 76-77 0.013438751655160536 78-79 0.0015907093795904307 80-81 0.002797454426176275 82-83 0.0010970409514416764 84-85 0.0 86-87 0.0015358573320183471 88-89 0.0040041994727621185 90-91 0.004113903567906286 92-93 0.012725675036723446 94-95 0.0 96-97 4.388163805766705E-4 98-99 0.0023037859980275205 100-101 0.0 102-103 5.4852047572083814E-5 104-105 0.0072404702795150645 106-107 0.0 108-109 0.0 110-111 3.2911228543250294E-4 112-113 0.006088577280501304 114-115 0.0018649696174508497 116-117 1.6455614271625147E-4 118-119 0.005156092471775879 120-121 0.0018101175698787661 122-123 0.0 124-125 0.07553126950675941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 911543.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.20306173036627 #Duplication Level Percentage of deduplicated Percentage of total 1 74.55548394635598 40.41135498681665 2 13.083139408233304 14.18292425942915 3 4.9551117937985705 8.057466913203895 4 2.4393058353366914 5.288713790879894 5 1.3490263695391633 3.656067979201158 6 0.8392166726952477 2.7292867869151864 7 0.5596738041382712 2.123522362820294 8 0.39592542096594135 1.7168296026590535 9 0.2824271087589351 1.3777572609350486 >10 1.4350887835688955 13.767341289558729 >50 0.07479484826773204 2.7266325660777526 >100 0.027968612902516336 2.8101737558116384 >500 0.0026347243359650437 0.9871475312782744 >1k 2.0267110276654183E-4 0.1647809144132441 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1500 0.16455614271625146 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 966 0.10597415590926594 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 939 0.10301214534037342 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.1940819028833528E-4 0.0 0.0 0.0 0.0 3 2.1940819028833528E-4 0.0 0.0 0.0 0.0 4 2.1940819028833528E-4 0.0 0.0 0.0 0.0 5 2.1940819028833528E-4 0.0 0.0 0.0 0.0 6 2.1940819028833528E-4 0.0 0.0 0.0 0.0 7 5.485204757208382E-4 0.0 0.0 0.0 0.0 8 5.485204757208382E-4 0.0 0.0 0.0 0.0 9 5.485204757208382E-4 0.0 0.0 0.0 0.0 10-11 5.485204757208382E-4 0.0 0.0 0.0 0.0 12-13 7.130766184370896E-4 0.0 0.0 0.0 0.0 14-15 9.873368562975087E-4 0.0 0.0 0.0 0.0 16-17 0.0011518929990137602 0.0 0.0 5.485204757208382E-5 0.0 18-19 0.0017004134747345984 0.0 0.0 1.0970409514416764E-4 0.0 20-21 0.0019746737125950174 0.0 0.0 2.742602378604191E-4 0.0 22-23 0.0019746737125950174 0.0 0.0 6.03372523292922E-4 0.0 24-25 0.002413490093171688 0.0 0.0 9.873368562975087E-4 0.0 26-27 0.002413490093171688 0.0 0.0 0.0013164491417300118 0.0 28-29 0.0026328982834600235 0.0 0.0 0.0034556789970412805 0.0 30-31 0.0028523064737483585 0.0 0.0 0.008721475563961327 0.0 32-33 0.0028523064737483585 0.0 0.0 0.019088512555085167 0.0 34-35 0.0030168626164646098 0.0 0.0 0.030771998687939024 0.0 36-37 0.003291122854325029 0.0 0.0 0.050463883766317115 0.0 38-39 0.003400826949469197 0.0 0.0 0.09143836330266372 0.0 40-41 0.003400826949469197 0.0 0.0 0.1446997014951571 0.0 42-43 0.003675087187329616 0.0 0.0 0.19209187059743754 0.0 44-45 0.00416875561547837 0.0 0.0 0.24052622860358752 0.0 46-47 0.004552719948482957 0.0 0.0 0.30113774117074016 0.0 48-49 0.004826980186343376 0.0 0.0 0.3722259948241608 0.0 50-51 0.004826980186343376 0.0 0.0 0.46201879669966195 0.0 52-53 0.004826980186343376 0.0 0.0 0.5441323119150715 0.0 54-55 0.004826980186343376 0.0 0.0 0.6286593172236526 0.0 56-57 0.0049366842814875436 0.0 0.0 0.7228951349524926 0.0 58-59 0.0049366842814875436 0.0 0.0 0.8357806488558411 0.0 60-61 0.004991536329059628 0.0 0.0 0.969235680598721 0.0 62-63 0.005046388376631712 0.0 0.0 1.1134965657133016 0.0 64-65 0.005046388376631712 0.0 0.0 1.243934734839717 0.0 66-67 0.005430352709636298 0.0 0.0 1.3884150281445855 0.0 68-69 0.00559490885235255 0.0 0.0 1.5579078551423247 0.0 70-71 0.005704612947496718 0.0 0.0 1.7523035117377896 0.0 72-73 0.005978873185357137 0.0 0.0 1.9572307614670947 0.0 74-75 0.006143429328073388 0.0 0.0 2.156288842106187 0.0 76-77 0.006143429328073388 0.0 0.0 2.3690599346383 0.0 78-79 0.006143429328073388 5.485204757208382E-5 0.0 2.6337759162211767 0.0 80-81 0.0069662100416546455 1.0970409514416764E-4 0.0 2.9293187485395644 0.0 82-83 0.007130766184370896 1.0970409514416764E-4 0.0 3.242414236081019 0.0 84-85 0.00718561823194298 1.0970409514416764E-4 0.0 3.5459654673449306 0.0 86-87 0.007295322327087147 1.0970409514416764E-4 0.0 3.851985040749586 0.0 88-89 0.007350174374659232 1.0970409514416764E-4 0.0 4.193439036885808 0.0 90-91 0.008008398945524237 1.0970409514416764E-4 0.0 4.5970404029212 0.0 92-93 0.00833751123095674 1.0970409514416764E-4 0.0 5.033169033166839 0.0 94-95 0.00833751123095674 1.0970409514416764E-4 0.0 5.461563524704814 0.0 96-97 0.00833751123095674 1.0970409514416764E-4 0.0 5.879426423108948 0.0 98-99 0.008556919421245077 1.0970409514416764E-4 0.0 6.333217412672798 0.0 100-101 0.00888603170667758 1.0970409514416764E-4 0.0 6.848332991422237 0.0 102-103 0.009269996039682166 1.0970409514416764E-4 0.0 7.436676053680408 0.0 104-105 0.0111898177047051 1.0970409514416764E-4 0.0 8.023428406558988 0.0 106-107 0.011464077942565518 1.0970409514416764E-4 0.0 8.57732438294189 0.0 108-109 0.011573782037709686 1.0970409514416764E-4 0.0 9.13665071203443 0.0 110-111 0.01162863408528177 1.0970409514416764E-4 0.0 9.785440730717037 0.0 112-113 0.01162863408528177 1.0970409514416764E-4 0.0 10.477344458791302 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1360 0.0 75.70792 1 GTATCAA 2815 0.0 56.87324 1 ATCAACG 3495 0.0 43.934597 3 TCAACGC 3510 0.0 43.57728 4 CAACGCA 3645 0.0 42.12659 5 TATCAAC 3810 0.0 41.7081 2 GTACATG 8275 0.0 40.276726 1 AACGCAG 3780 0.0 40.138702 6 TACATGG 8565 0.0 38.704544 2 ACATGGG 8565 0.0 36.758892 3 CATGGGA 5805 0.0 33.423313 4 CATGGGG 2835 0.0 33.169437 4 ACGCAGA 4625 0.0 32.801655 7 CGCAGAG 4765 0.0 31.589937 8 ATGGGAG 2120 0.0 30.600235 5 GTGGTAT 715 0.0 29.133732 1 ATGGGAT 2395 0.0 28.577644 5 TGGTATC 660 0.0 27.95451 2 GCAGAGT 5170 0.0 27.619247 9 GAGTACA 5935 0.0 26.574144 1 >>END_MODULE