FastQCFastQC Report
Fri 27 May 2016
SRR940124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911543
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56990.6252036382266114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43040.4721664255004975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.31155963020943606No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10240.11233699342762765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.082.516021
GTATCAA60050.066.9608841
ATCAACG76450.052.2704473
TCAACGC77200.051.6854934
TATCAAC77350.051.662262
CAACGCA78850.050.5678375
AACGCAG80900.049.3573046
GTGGTAT8050.048.8403171
TATAACG505.308208E-447.6432232
ACGCAGA94450.042.2018177
TGGTATC9100.041.8841552
CGCAGAG95600.041.694168
GCAGAGT102400.038.402539
GTACATG75900.035.239931
TACATGG77150.034.5050622
TAAGGTG6200.033.595235
ACATGGG78400.033.347223
TTACGTA902.3798879E-433.085574
TACCGAA452.3954391E-433.05109100-101
GAGTACT59700.032.08779512-13
Produced by FastQC (version 0.11.5)