FastQCFastQC Report
Fri 27 May 2016
SRR940123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395211
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18220.13058956673936772No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG16540.11854837727053472No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA14720.1055037553459656No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA14260.10220676299140417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.064.21351
GTATCAA28850.058.5866361
ATCAACG34750.046.4164243
TCAACGC35150.045.8882184
CAACGCA35650.045.244625
AACGCAG36500.043.6908156
TATCAAC39250.042.4565242
GTACATG76700.038.5644451
TACATGG79550.036.3598562
ACATGGG79200.035.54623
ACGCAGA45900.034.480267
CGCAGAG46100.034.3331268
CATGGGA41500.031.1220994
TAAGGTG8700.030.7858035
GTGTAGG7650.028.7850071
CATGGGG41650.028.5806584
GCAGAGT53250.028.3822489
GAGTGCA11150.027.7498829
GTAAGGT8650.026.147174
GGTAAGG8800.025.701483
Produced by FastQC (version 0.11.5)