FastQCFastQC Report
Fri 27 May 2016
SRR940123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395211
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.38180604940758067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.3181597622151775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26360.18893199666573732No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16720.11983850471362395No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG15380.11023422263729285No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG15110.10829903147265897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.080.694611
GTATCAA60850.063.6487961
ATCAACG81050.047.6232643
TCAACGC81950.047.1729354
TATCAAC82500.046.7862472
CAACGCA83800.046.017525
AACGCAG84750.045.429846
GTGGTAT10200.044.3967131
ACGCAGA98250.039.2988477
CGCAGAG99200.038.6826068
TGGTATC11350.036.7366332
GCAGAGT111200.034.1337139
GTACATG73950.034.083141
TACATGG77050.032.4693682
ACATGGG75600.031.3587283
GAGTACT69650.028.87109412-13
AGAGTAC104750.028.6249110-11
AGTACTT71500.027.95766412-13
CATGGGG41000.027.4583914
CAGAGTA109500.027.27452910-11
Produced by FastQC (version 0.11.5)