FastQCFastQC Report
Fri 27 May 2016
SRR940122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112182
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31890.1509813074820257No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30580.1447791904296126No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28380.134363421333957No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27330.1293922588110305No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24320.11514159291197446No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24100.11410001600240889No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21960.10396831333663481No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.065.1630861
GTATCAA42950.053.7680741
ATCAACG49850.045.3705833
TCAACGC50300.044.9646874
CAACGCA51400.043.8866125
AACGCAG52650.042.944496
TATCAAC54300.042.0908132
GTACATG107900.039.9919431
TACATGG117700.036.3081322
ACGCAGA64250.034.8165327
ACATGGG119950.034.3369563
CGCAGAG67100.032.9869888
TAAGGTG13250.032.7916225
CATGGGG60400.031.4347254
CATGGGA67050.027.784434
GCAGAGT77100.027.5522239
ATACAGT18650.027.118296
GTAAGGT14100.026.5936154
ATGGGAG30750.025.5496355
CCAATAC14000.025.0830253
Produced by FastQC (version 0.11.5)