FastQCFastQC Report
Fri 27 May 2016
SRR940122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112182
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.25002580270071423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40490.19169749576504297No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG30870.14615217817403994No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30470.14425840197482984No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27750.1313807238202011No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26140.12375827461838043No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24280.11495221529205343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.1135792275476261No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23440.11097528527371221No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21410.10136437106272092No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38550.079.730411
GTATCAA77300.064.189531
ATCAACG105150.046.8939483
TCAACGC105900.046.449374
TATCAAC106150.046.397182
CAACGCA108850.045.2130935
AACGCAG111050.044.315286
ACGCAGA126350.038.797657
CGCAGAG128550.037.994838
GTACATG104150.036.9463841
GCAGAGT141950.033.863319
TACATGG113300.033.8502582
ACATGGG112600.032.737693
TAAGGTG11950.031.373275
CATGGGA64200.028.941124
GTGGTAT15050.028.8924961
CATGGGG56600.027.6716524
GAGTACT85200.027.47632212-13
AGAGTAC139650.027.24288210-11
GTAAGGT13050.026.9238554
Produced by FastQC (version 0.11.5)