FastQCFastQC Report
Fri 27 May 2016
SRR940121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814072
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18610.22860385813539835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.066.5007861
GTATCAA24900.056.4147221
TCAACGC31450.042.772794
ATCAACG31950.042.2897153
CAACGCA32050.042.1577645
AACGCAG33400.040.260736
TATCAAC34550.039.968672
GTACATG79800.039.830721
TACATGG81050.038.7023542
ACATGGG83050.036.408593
CATGGGA57650.034.5879864
ACGCAGA42650.031.5250457
ATGGGAG20250.030.8634195
CGCAGAG43400.030.5708928
ATGGGAT25800.029.9918635
CATGGGG25350.027.9414394
CATGGGT6850.027.8060444
GGGACCG900.00950996126.454365
CGCACCT1358.2222876E-526.454365
GCAGAGT48100.026.3467779
Produced by FastQC (version 0.11.5)