FastQCFastQC Report
Fri 27 May 2016
SRR940121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814072
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67450.8285507915761751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.6420807987499877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33430.4106516376929804No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.15747992806533084No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.14802130524081408No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.11645161607327116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.080.1769261
TATTACG250.002047138871.483322
TACCGTG551.7283674E-764.901027
GTATCAA55500.061.5354771
ATCAACG71850.047.1717833
TCAACGC72550.046.7166444
TATCAAC73350.046.3723872
CAACGCA74850.045.23945
AACGCAG77400.043.900036
TGGTATC8350.040.664162
ACGCAGA88700.038.0967257
CGCAGAG89150.037.8376928
GTACATG72850.037.5532071
TACATGG73800.036.3228262
ACATGGG73250.035.454853
GCAGAGT96400.034.9920169
GTGGTAT10200.034.475911
GTACCGT700.00278685334.0041246
CATGGGT6950.033.4253964
CATGGGA52450.031.6851274
Produced by FastQC (version 0.11.5)