FastQCFastQC Report
Fri 27 May 2016
SRR940120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054269
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG12150.11524572950546777No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA12070.11448690988732477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.064.7256851
GTATCAA27750.061.550261
TCAACGC33850.048.8761634
ATCAACG34100.048.5178343
TATCAAC35350.047.475622
AACGCAG35900.046.076446
CAACGCA36300.045.5773545
GTACATG77850.039.063691
TACATGG79800.037.2887732
ACGCAGA45150.036.501397
CGCAGAG45300.036.2526258
ACATGGG82500.035.05853
CATGGGG32150.032.024044
GCAGAGT49450.031.1646359
CCCTATA1951.4169927E-930.5194282
GCGCGTC800.005361304629.7507939
CATGGGA54350.029.2363194
TATAGGG2252.055458E-1029.0951842
ATGGGAT21050.027.1412685
AGGTAAG4800.026.0368862
Produced by FastQC (version 0.11.5)