FastQCFastQC Report
Fri 27 May 2016
SRR940120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054269
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53810.5104010456534338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41750.3960089882183769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24880.2359929012424723No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA11520.10927002501259166No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.10784723822857355No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG10780.10225094354476894No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.083.746741
GTATCAA54000.061.9982151
TGGTATC8200.052.2940332
TCAACGC71300.046.858264
ATCAACG71650.046.7124863
TATCAAC72650.046.0716932
CAACGCA73450.045.452125
AACGCAG74400.044.8696256
ACGCAGA87650.038.145557
GTGGTAT11900.038.0454941
CGCAGAG89250.037.128428
GTACATG76900.036.2540171
TACATGG78300.035.140972
GCAGAGT97100.034.0042539
ACATGGG79000.033.4710043
TGTACCG1501.4761281E-731.7381675
GGACCGA2450.031.574746
CATGGGA51600.030.585044
TAAGGTG5100.030.3379545
TATATCG850.00720474528.0042635
Produced by FastQC (version 0.11.5)