FastQCFastQC Report
Fri 27 May 2016
SRR940119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723207
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25760.14948871493674296No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25590.14850218226829393No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22450.1302803435687065No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22190.1287715288993139No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19570.11356731953851162No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA18540.10759009219437944No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA17780.10317971085307802No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17280.10027814418116918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.065.862511
GTATCAA26650.051.3695871
TCAACGC31650.041.37254
ATCAACG31750.041.2421953
CAACGCA32950.040.1014825
AACGCAG35050.037.8587466
TATCAAC36750.036.278852
TACATGG67750.032.3296782
GTACATG70700.031.7393041
ACGCAGA42500.031.355977
CGCAGAG42450.031.2563848
ACATGGG68800.030.5385993
CATGGGG41800.026.4849724
TAAGGTG11050.025.3161935
CATGGGA29600.024.5318874
GAGTACA52750.023.2445011
AACCGAT1552.1086934E-423.0292997
GTAAGGT11150.022.9538974
GGTAAGG12200.022.4419633
GGACCGA4000.022.3141656
Produced by FastQC (version 0.11.5)