FastQCFastQC Report
Fri 27 May 2016
SRR940119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723207
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.16979968164010478No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25950.15059131027206832No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24680.1432213309254199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.14177054758946547No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22890.13283372223998627No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19660.11408960153945522No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA18940.10991134553190648No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18100.10503671352309968No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18050.10474655685590878No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA17380.10085845751555095No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.074.485081
GTATCAA45800.055.8150181
ATCAACG58950.042.847723
TCAACGC59200.042.6655354
CAACGCA61450.041.1620035
TATCAAC62300.040.7361372
AACGCAG63200.040.11646
TACATGG65450.033.132182
ACGCAGA76550.032.9513857
CGCAGAG76650.032.9083948
GTACATG68400.032.0590741
TGGTATC11800.031.3017022
TAAGGTG11700.031.0294045
ACATGGG66400.030.862853
GTAAGGT11600.027.2176694
GCAGAGT93750.026.6520759
CATGGGG41500.026.412094
GAGTACT54350.025.77771412-13
GGACCGT2109.792711E-825.5066056
AGAGTAC81000.025.19196710-11
Produced by FastQC (version 0.11.5)