FastQCFastQC Report
Fri 27 May 2016
SRR940118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089764
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15880.14571962369834204No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG15830.14526080876226413No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA13740.12608234443420777No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA13320.12222829897115339No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA12560.11525431194276926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11920.10938148076097211No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC11220.10295807165588147No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA11100.1018569158092945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.063.04511
GGTATCA15350.055.833031
ATCAACG31800.047.725463
TCAACGC32050.047.3531844
CAACGCA33450.045.5492135
AACGCAG34250.044.4791686
TATCAAC35400.044.0489042
GTACATG79700.041.3702851
TACATGG82150.039.4119952
ACATGGG81500.037.8276373
CGCAGAG42850.035.2712068
CATGGGA53950.035.0810624
ACGCAGA43200.034.982237
CATGGGG29000.030.5791444
ATGGGAT23450.029.948585
CCGTTGC1802.1971573E-829.75149
GCAGAGT49050.029.478459
GAGTACA55550.029.2493111
TATTCCG2103.1286618E-928.3411675
CCCTATA2353.4015102E-1027.8587682
Produced by FastQC (version 0.11.5)