FastQCFastQC Report
Fri 27 May 2016
SRR940118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089764
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56560.519011455691324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43650.4005454391960094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.2499623771752416No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15940.1462702016216355No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14460.13268927951372958No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA13820.12681644833193242No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA12920.11855777948253017No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA11680.10717916906779817No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC11170.10249925671980355No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA11020.10112281191156985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.076.104111
GTATCAA53450.063.531
ATCAACG71750.047.0601433
TCAACGC72650.046.477154
TATCAAC74150.045.621462
CAACGCA74600.045.1471945
AACGCAG76300.044.1412936
ACGCAGA88700.037.7622767
CGCAGAG89600.037.449378
GTACATG77300.036.9924771
TACATGG81000.034.9253352
ACATGGG79050.034.5782433
GCAGAGT97450.033.8221639
CATGGGA48900.032.8811454
TAAGGTA4250.032.227824
CATGGGG30000.030.1727184
TGGTATC9400.029.7784422
GAGTACT60450.028.68901612-13
GAGTACA58300.027.8962441
AGAGTAC94000.027.7849810-11
Produced by FastQC (version 0.11.5)