FastQCFastQC Report
Fri 27 May 2016
SRR940117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903374
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG20380.1070730187551159No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19930.10470879606425222No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG19760.10381564526992593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.070.582681
GTATCAA42550.055.6803051
TCAACGC50300.045.7995384
ATCAACG51100.045.0825163
CAACGCA52850.043.7023545
GTACATG124300.042.239191
AACGCAG54800.042.130646
TATCAAC58200.040.298732
TACATGG128100.040.0567972
ACATGGG132250.037.4944843
ACGCAGA69350.033.113717
CGCAGAG68950.033.052178
CATGGGG62950.032.8134424
CATGGGA71000.031.9436654
ATGGGAG28750.028.7802815
GCAGAGT76950.028.6887849
GAGTACA94150.028.0092531
ATGGGGG35350.027.1115635
AGTACAT92550.026.3709352
ATGGGGA31500.025.7007885
Produced by FastQC (version 0.11.5)