FastQCFastQC Report
Fri 27 May 2016
SRR940117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903374
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60570.31822437419025373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.25675458422779757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27790.14600388573133813No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG23340.12262435023279712No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG19720.10360549214184916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36750.085.424771
GTATCAA78350.061.357141
ATCAACG100900.047.223793
TCAACGC102150.046.585164
CAACGCA105450.045.142235
TATCAAC105700.045.135632
AACGCAG107200.044.348636
GTACATG115050.038.885161
TACATGG119000.036.9878852
CGCAGAG128500.036.8517658
ACGCAGA129250.036.7760057
ACATGGG120000.035.041723
GCAGAGT142750.032.714629
GTGGTAT13350.031.2354181
CATGGGG57500.031.0736734
CATGGGA66000.030.7715644
GAGTACT81350.028.77633712-13
TGGTATC14250.028.4220942
CAGAGTA139500.027.37846810-11
CGTACTA1107.760278E-427.041188
Produced by FastQC (version 0.11.5)