FastQCFastQC Report
Fri 27 May 2016
SRR940116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495789
Sequences flagged as poor quality0
Sequence length125
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52461.0581114143315No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22870.4612849417796684No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18810.3793952669381531No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT16240.32755869936606097No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT14570.29387501537952637No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG13960.28157139428264844No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13940.28116799686963606No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC13810.27854591368505555No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA13660.2755204330874626No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA12800.25817434432792985No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT12100.24405543487249617No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA11210.2261042499934448No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT11010.2220702758633209No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10080.20331229615824475No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA10010.20190040521270136No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA9800.1976647323760713No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG9750.1966562388435403No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG9540.1924205660069102No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC9480.19121037376787303No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT9450.19060527764835444No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC9380.18919338670281108No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA9090.1833441242141314No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG9080.18314242550762522No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG8480.17104050311725352No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC8430.17003200958472253No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT8390.16922521475869776No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT8380.16902351605219157No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG8290.1672082276936358No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT8260.1666031315741172No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG8190.16519124062857385No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG7730.15591310012928888No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA7670.1547029078902517No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG7540.15208082470567116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.15187912599916498No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC7460.1504672350536216No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA7300.14724005574952248No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC7140.14401287644542335No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT6910.13937380619578088No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT6730.13574322947866935No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6670.13453303723963217No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC6600.13312114629408883No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC6480.13070076181601448No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT6310.12727188380540916No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA6290.12686848639239676No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC6250.12606169156637198No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG6230.12565829415335958No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC6180.12464980062082863No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC6110.12323790967528525No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC6100.12303621096877906No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG6060.12222941614275429No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA6030.12162432002323569No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA6000.12101922390371711No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG5920.11940563425166753No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA5850.11799374330612418No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA5830.11759034589311178No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA5790.116783551067087No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG5710.11516996141503744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.11395976917600029No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA5650.11395976917600029No Hit
TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA5590.1127495769369631No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT5560.11214448081744452No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC5540.11174108340443213No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT5480.11053089116539495No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC5420.10932069892635779No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG5410.10911900021985158No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT5400.10891730151334539No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG5400.10891730151334539No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC5350.10790880798081443No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA5330.10750541056780204No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT5260.10609351962225866No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA5260.10609351962225866No Hit
CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT5130.10347143643767813No Hit
GGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAA5120.10326973773117194No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG5030.10145444937261618No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAG304.258698E-579.345038
GTATCAA8800.073.754381
GTTAGAG250.002050022671.453781
GGTATCA3100.069.148831
AGTGCCT359.1406124E-568.010039
GAACAAA8050.065.832161
TATGGTG1150.062.1337282
GTATGGT1200.059.5448231
CGCAGAA603.1331547E-759.5448232
CAGTGCC401.7697028E-459.5087748
CGCGTGC150.00423390759.50877458-59
CGAGTCC150.00423729159.49677336-37
GGGACCG401.7723531E-459.4907727
AGATCGC150.004238983659.4907732-33
GAGAACA4300.056.775291
GATCCTC752.2953827E-855.575175
TACAGGG752.2953827E-855.575172
ATCAACG11350.054.5608943
TCAACGC11250.053.9873054
ATGGGAC1550.053.7824175
Produced by FastQC (version 0.11.5)