FastQCFastQC Report
Fri 27 May 2016
SRR940116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495789
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85591.7263392289865245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64401.298939669899897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49040.9891304567063811No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.4612849417796684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.4261893668475904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17550.35398122991837255No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.32412982135545565No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT15160.3057752390633919No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT14310.28863084901036534No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA14160.28560536841277234No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA14000.28237818910867324No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG13420.27067966413131395No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT13410.27047796542480773No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC13100.2642253055231157No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT11820.2384078710903227No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT10910.22005328879825892No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG10870.21924649397223414No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA10680.21541421854861645No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT10570.2131955327770483No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA10560.2129938340705421No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG9420.19000018152883585No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.18677300222473672No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC9160.18475601515967477No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA9140.1843526177466624No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC8990.18132713714906945No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG8980.18112543844256326No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT8920.1799152462035261No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA8840.17830165655147653No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG8840.17830165655147653No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT8350.16841841993267295No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.1678133238131544No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT8320.1678133238131544No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT8020.16176236261796853No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.15631649754230126No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC7700.15530800400977027No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG7620.15369441435772072No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG7560.15248422211868357No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC7470.1506689337601278No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG7420.14966044022759684No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA7370.14865194669506585No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT7330.14784515186904107No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC7020.14159249196734902No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6940.13997890231529944No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA6920.13957550490228707No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.13655002430469412No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA6730.13574322947866935No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG6580.13271774888107643No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC6240.1258599928598658No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT6200.12505319803384102No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC6190.12485149932733482No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC6190.12485149932733482No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT6190.12485149932733482No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC6170.12444810191432243No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC6150.12404470450131003No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC6130.12364130708829764No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT6080.12263281355576668No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC6050.12202771743624809No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA6010.1212209226102233No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG6000.12101922390371711No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA6000.12101922390371711No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA5940.11980903166467992No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC5900.11900223683865516No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA5850.11799374330612418No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA5760.11617845494756843No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA5730.11557335882804982No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA5630.1135563717629879No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG5570.1123461795239507No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT5460.11012749375238257No Hit
CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT5420.10932069892635779No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT5410.10911900021985158No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC5380.108513904100333No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT5350.10790880798081443No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT5310.10710201315478962RNA PCR Primer, Index 27 (95% over 23bp)
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG5250.10589182091575247No Hit
TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA5210.1050850260897277No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG5150.10387483385069052No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT5120.10326973773117194No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG5090.10266464161165334No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT5010.10105105195960379No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA4960.1000425584270728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.098.79831
GTATCAA34000.062.3844571
GTCTATC300.00423780659.4905177
TGGGTAT1600.055.7723666
TGTATCG350.007798564650.991879
GTGGTAT3850.047.9740331
GGTACTC1750.047.6356543
ACTGATC255.331131E-447.6020294-95
CGAAAAC255.3337833E-447.59721890-91
GAGTCCT505.3338386E-447.5924156
GGTATGC1251.2732926E-1147.5924158
GTGGTAC1800.046.340511
CATGGGT2850.045.959594
ATCAACG46950.045.149873
ATGTATT802.2699696E-644.6854971
GGGTATG2000.044.617897
TCAACGC47650.044.4821134
GTATGGT951.4694888E-743.9015351
TGGTACT1900.043.8793752
CAACGCA48150.043.865565
Produced by FastQC (version 0.11.5)