FastQCFastQC Report
Fri 27 May 2016
SRR940115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522029
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36900.063.2359621
GGTATCA20500.058.3642161
TCAACGC44300.050.118184
ATCAACG44600.049.781063
CAACGCA46350.047.899945
TATCAAC48500.047.2507932
AACGCAG47600.046.628276
GTACATG107000.041.000411
TACATGG107350.039.368282
ACGCAGA59150.037.515947
CGCAGAG59700.036.5772138
ACATGGG111300.036.4736633
CATGGGA77050.032.9099434
GCAGAGT65600.031.6548679
CGTATAG1203.6859186E-529.7628461
ATGGGAG31250.029.1418635
GAGTACA78500.028.8908141
CATGGGG42250.028.599574
GTGTAAG6250.027.619921
AGTACAT78250.027.460792
Produced by FastQC (version 0.11.5)