FastQCFastQC Report
Fri 27 May 2016
SRR940115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522029
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68930.452882303819441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53410.3509131560568163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34480.22653970456541894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39000.071.9751361
GTATCAA72950.064.294771
ATCAACG97050.048.1884653
TCAACGC97200.048.1753964
TATCAAC97900.047.893362
CAACGCA101200.046.2195365
AACGCAG102600.045.587366
CGCAGAG120150.038.820558
ACGCAGA120650.038.80767
GTACATG106100.038.252331
TGGTATC11650.037.843312
TACATGG108950.036.637922
ACATGGG108300.035.096293
GCAGAGT131050.035.09239
CATGGGA71150.033.74424
ATGGGAG29150.031.2346295
GAGTACT77800.029.13498112-13
CAGAGTA130050.028.27141410-11
TAACGTG850.007205728.004295
AGAGTAC128650.027.46920210-11
Produced by FastQC (version 0.11.5)