FastQCFastQC Report
Fri 27 May 2016
SRR940114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537501
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG28550.11251227093112476No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG27830.10967483362568133No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27690.10912310970517844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.066.336341
GTATCAA49450.060.7900921
ATCAACG60750.048.5018273
TCAACGC61000.048.1078874
AACGCAG64050.046.0803956
CAACGCA64850.045.52635
TATCAAC66800.044.4655082
GTACATG141450.038.6320231
ACGCAGA80200.036.054187
TACATGG148450.035.92752
ACATGGG144900.035.534233
CGCAGAG82350.034.755068
CATGGGG70700.029.8045834
GCAGAGT94550.029.7670739
CATGGGA85600.029.0670994
TAAGGTG10700.026.1459675
ATAGGCG1150.001002805925.879915
ATGGGGA41300.025.6543245
GTATAGG8750.025.1710531
ATGGGAG40350.024.4881065
Produced by FastQC (version 0.11.5)