FastQCFastQC Report
Fri 27 May 2016
SRR940114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537501
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50780.20011814773669054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.15554673673035005No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG32280.1272117725273803No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG27900.10995069558593278No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26320.10372409705454302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.082.694051
GTATCAA80050.060.5097271
ATCAACG104150.046.3752483
TCAACGC105200.045.968994
TATCAAC108850.044.536972
CAACGCA109000.044.2196165
AACGCAG109400.044.0026746
GTACATG137450.037.8412551
ACGCAGA131450.036.5237437
TACATGG142850.035.8127442
CGCAGAG134850.035.5146268
ACATGGG140700.034.7515143
GCGTATC700.00277144334.045361
GCAGAGT148000.031.876739
CATGGGG71050.030.259924
CATGGGA77350.030.1051714
GAGTACT79950.026.93719912-13
ATGGGGA40050.026.5954275
GTATAGA5650.026.3625581
CAGAGTA145700.026.33676710-11
Produced by FastQC (version 0.11.5)