FastQCFastQC Report
Fri 27 May 2016
SRR940113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437556
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31920.7295066231522365No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10310.23562698260336962No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8940.20431670460466775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.16569307700042968No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6510.14878095603762717No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG5840.13346863030103576No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT5380.12295569024307745No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA4890.11175712365960014No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT4820.1101573284333891No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC4770.10901461755752408No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC4710.10764336450648604No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4710.10764336450648604No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG4680.106957737980967No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4550.10398668970371794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.1030725210030259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7500.093.656341
TCCGTTG851.4551915E-1162.992928
GTATCAA17200.061.949961
CCGTTGC954.1836756E-1156.368539
GTACTAG350.00777890451.023431
GTATGGT1450.049.2640041
ATCAACG21300.047.230613
TCAACGC21400.047.0099074
CAACGCA22250.045.4815645
AACGCAG22600.044.2251176
GTACATG38050.043.4917221
GCCTATC558.527326E-443.2678647
CGTCACT1404.0017767E-1142.5195241
GTCACTA1950.039.6848952
CATATAG1202.062552E-839.684891
ATAAGGG758.158211E-539.684893
CCGCTCA300.001308328439.6667448-49
ACAGTCC758.1857484E-539.662218
TATCAAC26550.039.4606822
TACATGG42100.039.1664432
Produced by FastQC (version 0.11.5)