##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940113_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 437556 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.503453272266864 33.0 31.0 34.0 25.0 34.0 2 30.525992101582425 34.0 31.0 34.0 25.0 34.0 3 30.596897311429853 34.0 31.0 34.0 25.0 34.0 4 33.381553446873085 37.0 35.0 37.0 23.0 37.0 5 33.843894267248075 37.0 35.0 37.0 28.0 37.0 6 33.396376692354806 37.0 35.0 37.0 22.0 37.0 7 33.51298119554983 37.0 35.0 37.0 26.0 37.0 8 33.250351954949764 37.0 35.0 37.0 20.0 37.0 9 34.507852709138945 39.0 37.0 39.0 17.0 39.0 10-11 34.74254381153498 39.0 37.0 39.0 20.0 39.0 12-13 34.66162616899322 39.0 37.0 39.0 18.5 39.0 14-15 35.987118220296374 41.0 38.0 41.0 17.5 41.0 16-17 36.15887337849327 41.0 38.0 41.0 23.5 41.0 18-19 36.26867075300076 41.0 38.0 41.0 24.5 41.0 20-21 36.40424882757864 41.0 38.0 41.0 25.5 41.0 22-23 36.51660701715895 41.0 38.0 41.0 27.0 41.0 24-25 36.66425097587509 41.0 37.5 41.0 28.0 41.0 26-27 36.70770255692986 41.0 37.0 41.0 29.0 41.0 28-29 36.65531497682582 40.5 37.0 41.0 29.0 41.0 30-31 36.5477870261178 40.0 37.0 41.0 29.0 41.0 32-33 36.457759692473644 40.0 37.0 41.0 29.0 41.0 34-35 36.33104219802723 40.0 36.5 41.0 28.5 41.0 36-37 36.146144722047005 40.0 36.0 41.0 27.5 41.0 38-39 35.95734374571484 40.0 36.0 41.0 27.0 41.0 40-41 35.81765648282734 40.0 35.5 41.0 27.0 41.0 42-43 35.72786797575625 40.0 35.5 41.0 26.5 41.0 44-45 35.75419717704705 40.0 36.0 41.0 26.5 41.0 46-47 35.65111208622439 40.0 35.0 41.0 26.0 41.0 48-49 35.50982159997805 40.0 35.0 41.0 25.5 41.0 50-51 35.37857439961971 40.0 35.0 41.0 25.0 41.0 52-53 35.21795724432987 40.0 35.0 41.0 25.0 41.0 54-55 35.048024709979984 40.0 35.0 41.0 25.0 41.0 56-57 34.851368739087114 40.0 35.0 41.0 24.0 41.0 58-59 34.58997248352211 39.0 35.0 41.0 22.5 41.0 60-61 34.35199837277971 39.0 35.0 41.0 19.5 41.0 62-63 34.05811256159211 39.0 34.0 41.0 13.5 41.0 64-65 33.74284548720621 38.5 34.0 41.0 5.0 41.0 66-67 33.40086754609696 37.5 34.0 40.0 2.0 41.0 68-69 33.05154540218852 37.0 33.5 40.0 2.0 41.0 70-71 32.89314167786523 37.0 34.0 39.5 2.0 41.0 72-73 32.626748347640074 36.0 34.0 39.0 2.0 41.0 74-75 32.26104772874787 36.0 34.0 39.0 2.0 41.0 76-77 31.897575396063587 35.0 33.5 37.5 2.0 40.0 78-79 31.54812641124793 35.0 33.0 37.0 2.0 39.0 80-81 31.212868752799643 35.0 33.0 37.0 2.0 39.0 82-83 30.895871614147676 35.0 33.0 36.0 2.0 38.0 84-85 30.601961806031685 35.0 33.0 36.0 2.0 37.0 86-87 30.386451105687044 35.0 33.0 36.0 2.0 37.0 88-89 30.18587449377908 35.0 33.0 35.0 2.0 36.5 90-91 30.021071588550953 35.0 32.5 35.0 2.0 36.0 92-93 29.86632796716306 35.0 32.0 35.0 2.0 36.0 94-95 29.739651610308165 35.0 32.0 35.0 2.0 36.0 96-97 29.61120976515006 35.0 32.0 35.0 2.0 36.0 98-99 29.51688469590178 35.0 32.0 35.0 2.0 35.5 100-101 29.440011107149715 35.0 32.0 35.0 2.0 35.0 102-103 28.7530396109298 34.0 29.5 35.0 2.0 35.0 104-105 29.242608946054904 35.0 31.0 35.0 2.0 35.0 106-107 29.28012528682043 35.0 31.0 35.0 2.0 35.0 108-109 29.23167777381638 35.0 31.0 35.0 2.0 35.0 110-111 29.130191335509053 35.0 31.0 35.0 2.0 35.0 112-113 29.024470010695772 35.0 31.0 35.0 2.0 35.0 114-115 28.88890907678103 35.0 31.0 35.0 2.0 35.0 116-117 28.795471893883295 35.0 31.0 35.0 2.0 35.0 118-119 28.64687148616406 35.0 30.0 35.0 2.0 35.0 120-121 28.468760570075602 35.0 30.0 35.0 2.0 35.0 122-123 28.241260547221387 35.0 30.0 35.0 2.0 35.0 124-125 26.370518287944858 33.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14193289010646382 1101 2 0.3326973708268248 1101 3 0.36335479641427426 1101 4 0.45083036551012867 1101 5 0.248985932676419 1101 6 0.35165529328268974 1101 7 0.29869710472626565 1101 8 0.39577418730880964 1101 9 0.4017884430747074 1101 10-11 0.44804122548524106 1101 12-13 0.4141236275808282 1101 14-15 0.44642319723451607 1101 16-17 0.3687598823832019 1101 18-19 0.2897957679645131 1101 20-21 0.14887575722645607 1101 22-23 0.16762583806016806 1101 24-25 0.06503223687850124 1101 26-27 0.11728364289756854 1101 28-29 0.326551232129205 1101 30-31 0.21696759828249412 1101 32-33 0.3193738998775828 1101 34-35 0.31991086949775394 1101 36-37 0.28888367238311474 1101 38-39 0.13131232002089632 1101 40-41 0.17294934926883343 1101 42-43 0.1117613549202261 1101 44-45 0.1876117599532492 1101 46-47 0.16023794550613957 1101 48-49 0.10480722918627805 1101 50-51 0.14623307901935334 1101 52-53 0.2663469364378699 1101 54-55 0.18817573134260357 1101 56-57 0.29012042589687326 1101 58-59 0.20047804758372934 1101 60-61 0.28792602693884817 1101 62-63 0.29159058453628717 1101 64-65 0.15549853575591754 1101 66-67 0.05988191110326824 1101 68-69 0.11827934077464874 1101 70-71 0.16662165261221418 1101 72-73 0.05768642291153725 1101 74-75 0.16904350434968762 1101 76-77 0.3591947476867965 1101 78-79 0.26861416634572066 1101 80-81 0.2752489299739942 1101 82-83 0.32699477499226504 1101 84-85 0.2795175120230553 1101 86-87 0.3372254910791632 1101 88-89 0.3651486133477171 1101 90-91 0.3135901389233702 1101 92-93 0.4102826255388088 1101 94-95 0.29312635865719017 1101 96-97 0.36835270388828434 1101 98-99 0.5209253360965853 1101 100-101 0.5171413945531 1101 102-103 0.3080339265610377 1101 104-105 0.2678700938145937 1101 106-107 0.4225169358330412 1101 108-109 0.2849536738576326 1101 110-111 0.16087114156002613 1101 112-113 0.3140797936418238 1101 114-115 0.2516597584172402 1101 116-117 0.18480673907068734 1101 118-119 0.10165937665922442 1101 120-121 0.20029242963463645 1101 122-123 0.10601902223836746 1101 124-125 0.1526281750344225 1102 1 -0.2109669558796199 1102 2 -0.2131639267123262 1102 3 -0.041946317657203025 1102 4 -0.13852581134731423 1102 5 -0.5467769983096105 1102 6 -0.1990512596160272 1102 7 -0.14156067185237475 1102 8 -0.38095903135722864 1102 9 -0.16596643871680072 1102 10-11 0.0339363049092114 1102 12-13 -0.3180340849190344 1102 14-15 -0.11716656365044997 1102 16-17 -0.5475273704357022 1102 18-19 -0.42305540063014035 1102 20-21 -0.5187239864584186 1102 22-23 -0.3687430871025583 1102 24-25 -0.30054284209578697 1102 26-27 -0.24845584874408644 1102 28-29 -0.3133400587772286 1102 30-31 -0.08504160882687728 1102 32-33 -0.16857900783772806 1102 34-35 -0.2700144281313257 1102 36-37 -0.2311521892516808 1102 38-39 -0.18967741613190725 1102 40-41 -0.21234822360673888 1102 42-43 -0.23978103263274875 1102 44-45 -0.3031516207084408 1102 46-47 -0.1971291472282246 1102 48-49 -0.15758555127081308 1102 50-51 -0.3169848562948019 1102 52-53 -0.31989804686792667 1102 54-55 -0.30302467786226117 1102 56-57 -0.2352736024102029 1102 58-59 -0.3980262187142074 1102 60-61 -0.32794696216775776 1102 62-63 -0.28610494006658627 1102 64-65 -0.5462540751734579 1102 66-67 -0.49290860748519805 1102 68-69 -0.4775788652442543 1102 70-71 -0.49104166397069804 1102 72-73 -0.4946361519313598 1102 74-75 -0.4732957084930476 1102 76-77 -0.49892274132425385 1102 78-79 -0.5309738183203407 1102 80-81 -0.5664835017596204 1102 82-83 -0.6223309925478944 1102 84-85 -0.6508178898183665 1102 86-87 -0.629693837105382 1102 88-89 -0.525224673669026 1102 90-91 -0.640940624513501 1102 92-93 -0.5466398503754384 1102 94-95 -0.5670736908071312 1102 96-97 -0.5981338114625174 1102 98-99 -0.5947340570660984 1102 100-101 -0.534741341273687 1102 102-103 -0.4927836400190664 1102 104-105 -0.4700462777524166 1102 106-107 -0.4882575743015245 1102 108-109 -0.38261515462482265 1102 110-111 -0.4954543899004058 1102 112-113 -0.560906153993443 1102 114-115 -0.39185282688061207 1102 116-117 -0.6627672606819921 1102 118-119 -0.5495524992752614 1102 120-121 -0.6760636235619977 1102 122-123 -0.7295823627627023 1102 124-125 -0.7335181606709504 1103 1 -0.3576113265729255 1103 2 -0.20524089332239726 1103 3 -0.4231420529835219 1103 4 -0.3846204611668682 1103 5 -0.4309915060492102 1103 6 -0.22830619302671806 1103 7 -0.3569272614500534 1103 8 -0.2273699199825785 1103 9 -0.592018601056516 1103 10-11 -0.21572029728683617 1103 12-13 -0.4590731796570182 1103 14-15 -0.08118018011989392 1103 16-17 -0.39242105220049694 1103 18-19 -0.2450004399182646 1103 20-21 -0.45910500871741533 1103 22-23 -0.3553185791638853 1103 24-25 -0.42777208394738153 1103 26-27 -0.41170560061606665 1103 28-29 -0.1410112386721778 1103 30-31 -0.09526249253232777 1103 32-33 0.015732476010491325 1103 34-35 0.09036557033298465 1103 36-37 0.0795965174965545 1103 38-39 0.12522761269075744 1103 40-41 0.11124910656420894 1103 42-43 0.06353663095399753 1103 44-45 -0.10033362581035732 1103 46-47 -0.09623560196939707 1103 48-49 -0.015669121356680193 1103 50-51 -0.13850758027122367 1103 52-53 0.06162331204889426 1103 54-55 0.024723286634809938 1103 56-57 -0.0271603138394525 1103 58-59 -0.19824974722261857 1103 60-61 -0.271100761725819 1103 62-63 -0.31250177995362804 1103 64-65 -0.34513956089293885 1103 66-67 -0.3304210138861947 1103 68-69 -0.3781698222932235 1103 70-71 -0.467716208168774 1103 72-73 -0.47006223537631797 1103 74-75 -0.4322543386557456 1103 76-77 -0.4029903345251178 1103 78-79 -0.4614737998351579 1103 80-81 -0.4665170594746222 1103 82-83 -0.5087736756862782 1103 84-85 -0.32256798153330735 1103 86-87 -0.21327387652148033 1103 88-89 -0.2734422755437045 1103 90-91 -0.24416188076646606 1103 92-93 -0.28754585180306336 1103 94-95 -0.33713509005009357 1103 96-97 -0.35245010145805367 1103 98-99 -0.3596508879664988 1103 100-101 -0.4025225222208668 1103 102-103 -0.28769338967282465 1103 104-105 -0.19519863831015627 1103 106-107 -0.19781128513071522 1103 108-109 -0.22986782999943145 1103 110-111 -0.37345076680473355 1103 112-113 -0.42944096159114054 1103 114-115 -0.522976754384576 1103 116-117 -0.47221916081650406 1103 118-119 -0.1726794526613169 1103 120-121 -0.2236601233216895 1103 122-123 -0.39741359589497094 1103 124-125 -0.5448157351306833 1104 1 0.2710142254827126 1104 2 0.29594646985787776 1104 3 0.26675689831548155 1104 4 0.167893746247735 1104 5 0.35214957453317197 1104 6 0.5123190969901543 1104 7 0.2525858377589145 1104 8 0.32591402632130695 1104 9 0.5228635633837371 1104 10-11 0.404252516190688 1104 12-13 0.5370303584123022 1104 14-15 0.6224034730281218 1104 16-17 0.5156879559281577 1104 18-19 0.4756867021668043 1104 20-21 0.29038183202306556 1104 22-23 0.2720977731472374 1104 24-25 0.29902520176768377 1104 26-27 0.32305793668897564 1104 28-29 0.37575924266029403 1104 30-31 0.4158632369453912 1104 32-33 0.4696873507504975 1104 34-35 0.5300065772169589 1104 36-37 0.536477491844046 1104 38-39 0.539828904323528 1104 40-41 0.6985298538114364 1104 42-43 0.7522100050853169 1104 44-45 0.6488188425402726 1104 46-47 0.4463279560803812 1104 48-49 0.6460279072328419 1104 50-51 0.618791291324321 1104 52-53 0.5940083015946414 1104 54-55 0.4302774167479768 1104 56-57 0.4281170378220338 1104 58-59 0.5558104889398194 1104 60-61 0.6567676074649711 1104 62-63 0.5673302422759932 1104 64-65 0.41980074339958406 1104 66-67 0.4651641787395988 1104 68-69 0.3828917622770547 1104 70-71 0.3615573439305422 1104 72-73 0.38059477225010596 1104 74-75 0.3581282925410605 1104 76-77 0.005979853105561261 1104 78-79 0.13966770656022476 1104 80-81 0.2336559952969388 1104 82-83 0.3359424216829616 1104 84-85 0.31462023327297217 1104 86-87 0.4232032851619003 1104 88-89 0.4610100907209862 1104 90-91 0.4418433381723865 1104 92-93 0.4716159516787748 1104 94-95 0.5953366998384837 1104 96-97 0.6267068979796555 1104 98-99 0.6627820874291537 1104 100-101 0.7360741509785704 1104 102-103 0.7561203116794033 1104 104-105 0.6449795480540033 1104 106-107 0.7142501411244773 1104 108-109 0.6780350955542609 1104 110-111 0.5660401137001259 1104 112-113 0.6516491196954703 1104 114-115 0.85197752530906 1104 116-117 0.6951867645351975 1104 118-119 0.6665938810723482 1104 120-121 0.8131563239784931 1104 122-123 0.6965259386586631 1104 124-125 0.5838766914029243 1105 1 -0.09082999173538298 1105 2 -0.106235394251879 1105 3 -0.11368526334033646 1105 4 -0.34734404325973856 1105 5 -0.22082920149684782 1105 6 -0.20575416859628604 1105 7 -0.02783053727331719 1105 8 0.04774477134422739 1105 9 -0.1370740224620377 1105 10-11 0.06946583471864898 1105 12-13 -0.27241044023626415 1105 14-15 -0.3341763260467232 1105 16-17 -0.28566294739304965 1105 18-19 -0.42228641659375654 1105 20-21 -0.3435231047997078 1105 22-23 -0.48406601927570136 1105 24-25 -0.47052395332570285 1105 26-27 -0.4755103794445006 1105 28-29 -0.530611632137763 1105 30-31 -0.6439038414301592 1105 32-33 -0.46723541041800587 1105 34-35 -0.4489615368336004 1105 36-37 -0.4294815337373379 1105 38-39 -0.07239158709583648 1105 40-41 -0.2564119684082087 1105 42-43 -0.3384220829470621 1105 44-45 -0.28175988296900556 1105 46-47 -0.3941357295122643 1105 48-49 -0.37837992137556853 1105 50-51 -0.2467564639237878 1105 52-53 -0.29228452919774384 1105 54-55 -0.24259295021146698 1105 56-57 -0.23099827048599764 1105 58-59 -0.21786189463883687 1105 60-61 -0.3162353764157544 1105 62-63 -0.17447066393963695 1105 64-65 -0.03794603195682811 1105 66-67 -0.31039483842166504 1105 68-69 -0.399577017489392 1105 70-71 -0.33808374836978317 1105 72-73 -0.41947995633252333 1105 74-75 -0.45967605539942724 1105 76-77 -0.6603504472296642 1105 78-79 -0.606689275036544 1105 80-81 -0.5688000490350049 1105 82-83 -0.5424197067133747 1105 84-85 -0.4441380092370615 1105 86-87 -0.2916821748661995 1105 88-89 -0.4512061906373894 1105 90-91 -0.42834600932078715 1105 92-93 -0.4403569355284276 1105 94-95 -0.35595947268981476 1105 96-97 -0.2828530980207873 1105 98-99 -0.3264230530553185 1105 100-101 -0.2612708793075562 1105 102-103 -0.1581220615319765 1105 104-105 -0.3099385925725606 1105 106-107 -0.39006201384786365 1105 108-109 -0.24790861593026037 1105 110-111 -0.18265608303602576 1105 112-113 -0.14991228667540213 1105 114-115 -0.07183484279762453 1105 116-117 0.17003559703265836 1105 118-119 0.004928854575279473 1105 120-121 0.04764356486239407 1105 122-123 -0.009055286574341181 1105 124-125 0.020974433231458534 1106 1 -0.15379129261706836 1106 2 -0.16423919552952881 1106 3 0.07762195380765036 1106 4 -0.15125883128571616 1106 5 -0.005354833723238528 1106 6 -0.2685438840284178 1106 7 -0.3093890611104939 1106 8 0.015074901749166258 1106 9 0.13922078773805424 1106 10-11 0.09514557707649374 1106 12-13 -0.03725654128690081 1106 14-15 0.3583359489123623 1106 16-17 0.2960764011101986 1106 18-19 0.20716424599005023 1106 20-21 0.26279651929616676 1106 22-23 0.37473235462219634 1106 24-25 0.20864557309845821 1106 26-27 0.3070974554110961 1106 28-29 0.19935378679471683 1106 30-31 0.3206967604388069 1106 32-33 0.07035896157925237 1106 34-35 0.11757270106303253 1106 36-37 0.11779685705512577 1106 38-39 -0.1334311309425189 1106 40-41 -0.28599198002322623 1106 42-43 -0.024366594852452295 1106 44-45 -0.026352301531915145 1106 46-47 0.12095455083196782 1106 48-49 0.10228503823585555 1106 50-51 0.04302892227546806 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-0.04742400283673476 2216 16-17 -0.17523668500210476 2216 18-19 -0.11709253442941758 2216 20-21 -0.20853310442460327 2216 22-23 -0.4885308073685479 2216 24-25 -0.46676397591122765 2216 26-27 -0.5193014317051308 2216 28-29 -0.6312514896751935 2216 30-31 -0.389525427194954 2216 32-33 -0.4758461436777637 2216 34-35 -0.39564863050629384 2216 36-37 -0.29918434843924757 2216 38-39 -0.2866530461921428 2216 40-41 -0.3376389312664685 2216 42-43 -0.3730367765715883 2216 44-45 -0.26254145286076636 2216 46-47 -0.201788552012502 2216 48-49 -0.4351181681145846 2216 50-51 -0.3526547619145788 2216 52-53 -0.2864436821614049 2216 54-55 -0.2955011333200943 2216 56-57 -0.2838659812727542 2216 58-59 -0.26640005411557866 2216 60-61 -0.12226535752208889 2216 62-63 -0.22170877346761841 2216 64-65 -0.34448932056002235 2216 66-67 -0.00913368138707682 2216 68-69 0.1858640024920959 2216 70-71 0.005433474083865519 2216 72-73 -0.069723086402675 2216 74-75 0.015211362446692078 2216 76-77 -0.11849022086105876 2216 78-79 -0.09325345130633877 2216 80-81 0.15423205834892428 2216 82-83 0.0951930975447084 2216 84-85 0.12802808917188102 2216 86-87 0.01943843462722583 2216 88-89 0.043297470627042145 2216 90-91 0.18429734660465513 2216 92-93 0.031062695324511225 2216 94-95 -0.06958423306405592 2216 96-97 0.1123324725106265 2216 98-99 0.11762346839797289 2216 100-101 -0.052750567137628934 2216 102-103 -0.04422702501804565 2216 104-105 0.036983240566968334 2216 106-107 -0.06499227312738043 2216 108-109 -0.012382406646896271 2216 110-111 -0.07379442138068626 2216 112-113 0.046986546339745416 2216 114-115 0.13134114862619484 2216 116-117 0.22353901941168175 2216 118-119 0.08993060179502876 2216 120-121 -0.1109299536038435 2216 122-123 -0.10687392713866117 2216 124-125 -0.39241578510191033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11639.0 3 2194.0 4 2129.0 5 1821.0 6 3142.0 7 3155.0 8 2798.0 9 2641.0 10 3042.0 11 3383.0 12 3134.0 13 2811.0 14 2572.0 15 2421.0 16 1976.0 17 1656.0 18 1522.0 19 1266.0 20 1191.0 21 1165.0 22 1259.0 23 2146.0 24 3755.0 25 3747.0 26 5194.0 27 3999.0 28 3500.0 29 4690.0 30 5072.0 31 5626.0 32 7371.0 33 10163.0 34 15247.0 35 25403.0 36 52757.0 37 141649.0 38 90046.0 39 274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38360182054676 22.293011102957816 13.385270578876263 20.93811649761916 2 14.106807345405006 24.542283621867984 40.180983684496844 21.169925348230166 3 16.14073342658877 34.760735803688156 28.677647037311317 20.420883732411752 4 10.72433747390707 21.012668646642844 38.55100496365852 29.711988915791565 5 10.84616105660964 44.43928769841723 32.66834172003137 12.046209524941757 6 25.271522331011496 42.51087575238718 18.985646064269236 13.231955852332087 7 25.57690373661739 37.550627645665905 22.53855929491575 14.333909322800956 8 25.22454371346165 37.95508668644327 20.632881884293138 16.187487715801936 9 27.762768116737067 19.86166730667252 19.837895660760328 32.53766891583009 10-11 22.00500508507502 33.00442230119642 26.590714310201 18.399858303527555 12-13 23.400051424163642 31.057337942462077 26.48218724109362 19.06042339228066 14-15 22.146307120145746 31.203811857537655 24.652496016933462 21.99738500538314 16-17 19.895417786061266 34.320495577081964 25.757483993276203 20.026602643580567 18-19 20.37452258997936 32.70501489084995 27.866117202303 19.05434531686769 20-21 22.321628552230415 31.65570390956754 27.13316664457355 18.889500893628497 22-23 22.109195290654558 31.599762772951088 26.82699680383403 19.464045132560326 24-25 21.207733553969856 32.02273257116214 27.251429642395124 19.518104232472883 26-27 20.263738272902835 32.100650203974354 27.415182091394225 20.220429431728583 28-29 20.690427540306175 30.86410864725753 28.00279297687452 20.44267083556177 30-31 20.781325169722876 31.908701032906983 27.301129124228808 20.008844673141333 32-33 20.367593955406036 32.12813222777844 27.020235494162925 20.4840383226526 34-35 20.611556705377946 31.743630236621414 27.643007572753625 20.001805485247015 36-37 20.698048886963896 31.744527958182434 26.775949414826083 20.781473740027586 38-39 20.42401914291134 32.08974496687244 27.165976080502613 20.320259809713612 40-41 20.155338208033427 31.853361280191635 27.610360756759473 20.38093975501546 42-43 20.060225129992574 31.674990000571395 27.46848751499914 20.79629735443689 44-45 20.078550753454163 31.799456749662614 27.415515659299533 20.70647683758369 46-47 20.352592673862063 31.955396775329024 27.119990308615733 20.57202024219318 48-49 19.632033642638387 32.23225305114961 26.97456232573022 21.161150980481786 50-51 20.1738677284171 31.839827755067525 26.954412005032953 21.031892511482425 52-53 20.119373034447452 31.873948657939007 27.26701345717838 20.73966485043516 54-55 20.121931012415935 32.03185976219453 27.140563488118296 20.705645737271237 56-57 20.15453202122444 32.313443325878346 26.544148822496165 20.987875830401048 58-59 19.820629135250478 31.590484909371654 27.359714359841224 21.229171595536645 60-61 19.991429501939788 32.62262954307818 26.55083162399511 20.83510933098692 62-63 20.111581175190672 32.17958123918643 27.153514395617208 20.55532319000569 64-65 20.122227148688367 32.03491747153443 27.038071425632964 20.80478395414425 66-67 19.85558205414473 32.43278760460245 26.345933431531748 21.36569690972107 68-69 20.27902746051644 32.46753840369965 26.606339081556513 20.647095054227407 70-71 20.545058521567615 32.151093293960884 26.761075215241892 20.54277296922961 72-73 20.33289567356752 32.629535292175035 26.062010560970116 20.97555847328733 74-75 20.19781613727137 33.090377176291774 26.095502568409984 20.616304118026868 76-77 20.060504378395798 32.640706394903646 27.16056860858216 20.1382206181184 78-79 20.402491603807114 32.416903687468505 26.683495238824406 20.497109469899975 80-81 20.21685072771155 33.058308344913335 26.197387186425804 20.527453740949316 82-83 20.968256163016985 32.3655952069423 26.211091545070904 20.455057084969816 84-85 20.56845295230782 32.10503341286601 26.565970984285443 20.76054265054073 86-87 20.182083504267545 32.51323002240905 26.727216421568905 20.5774700517545 88-89 20.887243306249786 31.62444147325357 26.87602134686368 20.612293873632968 90-91 20.825624435045842 31.916724471500025 26.740915879968597 20.516735213485536 92-93 20.867223848255826 31.986893699307085 26.537744614565273 20.608137837871816 94-95 20.56902430774575 31.474942635914033 26.923868030606368 21.03216502573385 96-97 20.949005034818637 31.686675671290104 26.494961753204755 20.8693575406865 98-99 20.307259473586175 32.08717721928348 26.531983530855648 21.073579776274695 100-101 19.89653895729918 32.21987585589044 26.79474170163362 21.088843485176756 102-103 20.384408817177267 32.35147581446904 26.38925392236405 20.874861445989648 104-105 19.486328531234214 33.25322216051033 26.494170512231523 20.766278796023926 106-107 19.647656528535776 33.233574673870315 26.4215323295761 20.697236468017806 108-109 19.701021126438672 33.76824909268756 25.32418707548291 21.206542705390852 110-111 19.415384439581537 34.2930276938196 25.473171790813677 20.818416075785194 112-113 19.240460907118052 34.39158820511121 26.146982425144877 20.220968462625862 114-115 19.443025534662347 34.95534719484393 25.467468874452198 20.134158396041528 116-117 19.320885374410075 34.822479459725066 25.601238701420392 20.25539646444447 118-119 18.99828926602999 34.64390563879401 25.512905927916048 20.844899167259957 120-121 19.03781875110702 34.644581445443066 25.46329255680812 20.854307246641792 122-123 19.180995325164467 34.73159404921204 25.5981241236826 20.489286501940896 124-125 19.405557220063443 34.97037048896126 25.12516209892666 20.498910192048633 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2449.0 1 2250.0 2 2450.0 3 2349.0 4 1440.0 5 863.5 6 637.5 7 593.5 8 700.5 9 706.0 10 525.5 11 404.5 12 372.5 13 370.0 14 361.5 15 349.5 16 366.5 17 417.0 18 480.5 19 553.5 20 673.0 21 827.0 22 972.5 23 1170.0 24 1389.0 25 1778.5 26 2299.0 27 2725.5 28 3115.0 29 3916.0 30 5327.0 31 6541.5 32 8485.5 33 11337.5 34 13986.0 35 15801.5 36 19500.0 37 22685.0 38 22877.5 39 23381.0 40 21560.5 41 18770.5 42 17006.5 43 15878.0 44 15443.5 45 15544.0 46 14549.5 47 12569.0 48 11151.0 49 10077.0 50 9614.5 51 8628.5 52 8038.0 53 8051.0 54 7810.5 55 7304.5 56 6791.0 57 6254.5 58 5894.5 59 5643.5 60 5085.5 61 4888.5 62 4318.0 63 2634.0 64 1486.5 65 1293.5 66 1099.0 67 825.5 68 594.5 69 461.5 70 382.0 71 289.0 72 251.5 73 309.0 74 269.0 75 157.5 76 103.0 77 69.0 78 53.0 79 32.5 80 36.0 81 34.0 82 18.0 83 5.0 84 5.0 85 6.0 86 3.5 87 3.0 88 1.5 89 2.5 90 2.5 91 0.5 92 1.0 93 1.5 94 2.0 95 5.5 96 6.0 97 3.5 98 3.5 99 6.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023996928393165674 2 0.013255446160034373 3 0.010970024408304309 4 0.040451965005622134 5 0.03588112150216201 6 0.026282350144895737 7 0.010970024408304309 8 4.570843503460129E-4 9 0.013712530510380386 10-11 2.2854217517300645E-4 12-13 0.004228030240700619 14-15 0.01805483183866751 16-17 0.0012569819634515355 18-19 0.00891314483174725 20-21 0.00319959045242209 22-23 1.1427108758650323E-4 24-25 0.007770433955882218 26-27 2.2854217517300645E-4 28-29 0.006856265255190193 30-31 0.001028439788278529 32-33 0.0018283374013840516 34-35 1.1427108758650323E-4 36-37 0.008341789393814735 38-39 0.001142710875865032 40-41 0.01348398833520738 42-43 0.007084807430363199 44-45 0.001028439788278529 46-47 0.012341277459342347 48-49 0.0036566748027681032 50-51 0.009027415919333755 52-53 0.004570843503460128 54-55 0.0030853193648355867 56-57 0.009484500269679766 58-59 0.01885472945177303 60-61 0.0019426084889705545 62-63 0.006170638729671173 64-65 0.002285421751730064 66-67 0.0014855241386245418 68-69 1.1427108758650323E-4 70-71 0.005713554379325161 72-73 3.4281326275950965E-4 74-75 0.006513451992430683 76-77 0.01531232573659143 78-79 0.0014855241386245418 80-81 0.004570843503460128 82-83 0.0014855241386245418 84-85 0.0 86-87 0.002171150664143561 88-89 0.004799385678633135 90-91 0.0046851145910466315 92-93 0.012912632897274864 94-95 0.0 96-97 6.856265255190193E-4 98-99 0.0023996928393165673 100-101 0.0 102-103 2.2854217517300645E-4 104-105 0.00891314483174725 106-107 0.0 108-109 0.0 110-111 7.998976131055224E-4 112-113 0.0062849098172576764 114-115 0.0030853193648355867 116-117 2.2854217517300645E-4 118-119 0.005599283291738658 120-121 0.0019426084889705545 122-123 1.1427108758650323E-4 124-125 0.07381912258088108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 437556.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.515243896086254 #Duplication Level Percentage of deduplicated Percentage of total 1 71.93332271280084 24.82796177688202 2 10.955860325159666 7.562883824286814 3 4.897531242131988 5.071184559326635 4 2.7886431873823048 3.8500279900663847 5 1.8645434946583792 3.2177586736497483 6 1.249047702788642 2.586671165975774 7 0.9408840870540485 2.273239061983186 8 0.7106413365282606 1.96223672423309 9 0.5259790094162029 1.6338864414799883 >10 3.5343618019728082 23.45652573499375 >50 0.38069771553749626 9.11724474825441 >100 0.21385280368454138 12.700202875629424 >500 0.0033104149177173585 0.7751383358668311 >1k 0.0013241659670869435 0.9650380873719422 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3192 0.7295066231522365 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1031 0.23562698260336962 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 894 0.20431670460466775 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 725 0.16569307700042968 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 651 0.14878095603762717 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 584 0.13346863030103576 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 538 0.12295569024307745 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 489 0.11175712365960014 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 482 0.1101573284333891 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 477 0.10901461755752408 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 471 0.10764336450648604 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 471 0.10764336450648604 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 468 0.106957737980967 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 455 0.10398668970371794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 451 0.1030725210030259 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2854217517300643E-4 0.0 16-17 0.0 0.0 0.0 2.2854217517300643E-4 0.0 18-19 0.0 0.0 0.0 5.71355437932516E-4 0.0 20-21 0.0 0.0 0.0 0.0013712530510380386 0.0 22-23 0.0 0.0 0.0 0.004342301328287122 0.0 24-25 0.0 0.0 0.0 0.009141687006920257 0.0 26-27 0.0 0.0 0.0 0.014169614860726397 0.0 28-29 0.0 0.0 0.0 0.025025368181444206 0.0 30-31 0.0 0.0 0.0 0.05873533901946265 0.0 32-33 0.0 0.0 0.0 0.10787190668165902 0.0 34-35 0.0 0.0 0.0 0.159865251533518 0.0 36-37 0.0 0.0 0.0 0.2454542961358089 0.0 38-39 0.0 0.0 0.0 0.38909305323204346 0.0 40-41 0.0 0.0 0.0 0.5646134437649124 0.0 42-43 0.0 0.0 0.0 0.7197935807073838 0.0 44-45 0.0 0.0 0.0 0.8846867600947079 0.0 46-47 0.0 0.0 0.0 1.0790618800793497 0.0 48-49 0.0 0.0 0.0 1.2917203740778322 0.0 50-51 0.0 0.0 0.0 1.5472305259212535 0.0 52-53 0.0 0.0 0.0 1.7795436469846146 0.0 54-55 0.0 0.0 0.0 1.996315900136211 0.0 56-57 0.0 0.0 0.0 2.2375421660313193 0.0 58-59 0.0 0.0 0.0 2.50287963140718 0.0 60-61 0.0 0.0 0.0 2.8359798517218366 0.0 62-63 0.0 0.0 0.0 3.170337053999945 0.0 64-65 0.0 0.0 0.0 3.4708700143524487 0.0 66-67 0.0 0.0 0.0 3.7798590351863535 0.0 68-69 0.0 0.0 0.0 4.151468612017662 0.0 70-71 0.0 0.0 0.0 4.579870919379463 0.0 72-73 0.0 0.0 0.0 5.0198146065875 0.0 74-75 0.0 0.0 0.0 5.435875636489958 0.0 76-77 0.0 0.0 0.0 5.862563877537961 0.0 78-79 0.0 0.0 0.0 6.345702035853696 0.0 80-81 0.0 0.0 0.0 6.908601413304812 0.0 82-83 0.0 0.0 0.0 7.49846876742634 0.0 84-85 0.0 0.0 0.0 8.079080163453362 0.0 86-87 0.0 0.0 0.0 8.674661071954219 0.0 88-89 0.0 0.0 0.0 9.283497426615108 0.0 90-91 0.0 0.0 0.0 9.984779091133479 0.0 92-93 0.0 0.0 0.0 10.767421770013438 0.0 94-95 0.0 0.0 0.0 11.544579436689247 0.0 96-97 0.0 0.0 0.0 12.295340482132573 0.0 98-99 0.0 0.0 0.0 13.077183263399426 0.0 100-101 0.0 0.0 0.0 13.940615601203046 0.0 102-103 0.0 0.0 0.0 14.885408953368255 0.0 104-105 0.0 0.0 0.0 15.831002203146568 0.0 106-107 0.0 0.0 0.0 16.765396886341406 0.0 108-109 0.0 0.0 0.0 17.676480267668595 0.0 110-111 0.0 0.0 0.0 18.691093254349155 0.0 112-113 0.0 0.0 0.0 19.751528947151908 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 750 0.0 93.65634 1 TCCGTTG 85 1.4551915E-11 62.99292 8 GTATCAA 1720 0.0 61.94996 1 CCGTTGC 95 4.1836756E-11 56.36853 9 GTACTAG 35 0.007778904 51.02343 1 GTATGGT 145 0.0 49.264004 1 ATCAACG 2130 0.0 47.23061 3 TCAACGC 2140 0.0 47.009907 4 CAACGCA 2225 0.0 45.481564 5 AACGCAG 2260 0.0 44.225117 6 GTACATG 3805 0.0 43.491722 1 GCCTATC 55 8.527326E-4 43.267864 7 CGTCACT 140 4.0017767E-11 42.519524 1 GTCACTA 195 0.0 39.684895 2 CATATAG 120 2.062552E-8 39.68489 1 ATAAGGG 75 8.158211E-5 39.68489 3 CCGCTCA 30 0.0013083284 39.66674 48-49 ACAGTCC 75 8.1857484E-5 39.66221 8 TATCAAC 2655 0.0 39.460682 2 TACATGG 4210 0.0 39.166443 2 >>END_MODULE