FastQCFastQC Report
Fri 27 May 2016
SRR940113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437556
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68731.5707703699640732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53921.2322994085328507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36260.8286939271773214No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13130.30007587600215746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.28202104416348994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10670.24385450090959787No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT10150.23197030780060152No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.20888754810812785No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.20820192158260883No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6610.15106637778935725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5570.12729799157136457No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT5560.12706944939619158No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG5310.12135589501686642No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.12021318414100139No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.11929901544030935No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5080.11609942498788726No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT4850.11084295495890813TruSeq Adapter, Index 11 (95% over 21bp)
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT4820.1101573284333891No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC4590.10490085840440995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4530.10352960535337191No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4510.1030725210030259No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA4480.10238689447750687No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.10238689447750687No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG4450.10170126795198786No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4430.10124418360164184No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4420.10101564142646885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.096.914381
GTATCAA46000.061.892651
TGGGTAT803.832247E-852.075016
AGGCTCG350.007785646751.012255
ATCAACG61550.045.8585053
TCAACGC62250.045.342834
CAACGCA63350.044.436095
TATCAAC64150.043.9998632
AACGCAG64600.043.5762566
ACGCAGA73000.038.3770077
CGCAGAG73250.038.1648258
GTTATAT801.1889491E-437.226231
TGGTATC7100.036.9031942
GTACATG35350.035.720331
GCAGAGT77800.035.397659
GTATGGT1703.2196112E-1035.036451
CATATAG1407.9890015E-834.027623
GATAGTC1051.48495765E-533.988747
ACATGGG36250.033.5113373
ATGGGAT11450.032.7458535
Produced by FastQC (version 0.11.5)