##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940113_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 437556 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66722430957409 34.0 31.0 34.0 31.0 34.0 2 32.76117342694421 34.0 31.0 34.0 31.0 34.0 3 32.83873607035442 34.0 31.0 34.0 31.0 34.0 4 36.27465055901416 37.0 37.0 37.0 35.0 37.0 5 36.26862847269835 37.0 37.0 37.0 35.0 37.0 6 36.3470321513132 37.0 37.0 37.0 35.0 37.0 7 36.308904460229094 37.0 37.0 37.0 35.0 37.0 8 36.31791816361791 37.0 37.0 37.0 35.0 37.0 9 38.117038733327846 39.0 39.0 39.0 37.0 39.0 10-11 38.09865708617868 39.0 39.0 39.0 36.0 39.0 12-13 38.10638523983216 39.0 39.0 39.0 36.0 39.0 14-15 39.48737647295432 41.0 39.0 41.0 36.5 41.0 16-17 39.495593706862664 41.0 39.0 41.0 36.5 41.0 18-19 39.62513141175073 41.0 40.0 41.0 37.0 41.0 20-21 39.61157428991946 41.0 40.0 41.0 37.0 41.0 22-23 39.53541946630831 41.0 40.0 41.0 36.0 41.0 24-25 39.487363903134685 41.0 39.5 41.0 36.0 41.0 26-27 39.30795601020212 41.0 39.0 41.0 35.0 41.0 28-29 39.15543610417866 41.0 39.0 41.0 35.0 41.0 30-31 38.80649676841364 40.5 38.0 41.0 35.0 41.0 32-33 38.37977995959375 40.0 37.0 41.0 35.0 41.0 34-35 38.123437914232696 40.0 37.0 41.0 35.0 41.0 36-37 37.90091896808637 40.0 36.0 41.0 34.0 41.0 38-39 37.66469320498405 40.0 36.0 41.0 33.5 41.0 40-41 37.46128381281481 40.0 36.0 41.0 33.0 41.0 42-43 37.14072827249541 40.0 36.0 41.0 33.0 41.0 44-45 36.90874539487517 40.0 36.0 41.0 32.5 41.0 46-47 36.20303801113457 40.0 36.0 41.0 28.0 41.0 48-49 35.553709696587404 40.0 35.5 41.0 22.0 41.0 50-51 35.276394335810735 40.0 35.0 41.0 20.0 41.0 52-53 35.17353892987412 40.0 35.0 41.0 19.5 41.0 54-55 35.017629743392845 40.0 35.0 41.0 18.0 41.0 56-57 34.87702716909378 40.0 35.0 41.0 17.5 41.0 58-59 34.674424530802916 39.5 35.0 41.0 15.0 41.0 60-61 34.43580364570478 39.0 35.0 41.0 11.0 41.0 62-63 34.19797351653274 39.0 35.0 41.0 7.5 41.0 64-65 33.89773309016446 38.5 35.0 41.0 6.0 41.0 66-67 33.584588029875036 37.5 35.0 40.5 2.0 41.0 68-69 33.215229593469175 37.0 35.0 40.0 2.0 41.0 70-71 32.90108580387425 37.0 34.5 39.5 2.0 41.0 72-73 32.662252374553205 36.0 35.0 39.0 2.0 41.0 74-75 32.33398924937608 36.0 34.0 39.0 2.0 41.0 76-77 31.995248608178155 35.0 34.0 37.5 2.0 40.0 78-79 31.657559260986023 35.0 34.0 37.0 2.0 39.0 80-81 31.34363944272276 35.0 34.0 37.0 2.0 39.0 82-83 31.039948029509368 35.0 34.0 36.0 2.0 38.0 84-85 30.769928877675085 35.0 34.0 36.0 2.0 37.0 86-87 30.571147464553107 35.0 34.0 36.0 2.0 37.0 88-89 30.374421788296814 35.0 33.5 35.0 2.0 36.5 90-91 30.232900474453555 35.0 33.0 35.0 2.0 36.0 92-93 30.086992293557856 35.0 33.0 35.0 2.0 36.0 94-95 29.9638366289115 35.0 33.0 35.0 2.0 36.0 96-97 29.87716657982064 35.0 33.0 35.0 2.0 36.0 98-99 29.80349829507537 35.0 33.0 35.0 2.0 35.5 100-101 29.74641874411504 35.0 33.0 35.0 2.0 35.0 102-103 29.029725338013876 34.5 31.5 35.0 2.0 35.0 104-105 29.561008190951558 35.0 33.0 35.0 2.0 35.0 106-107 29.584535465174746 35.0 33.0 35.0 2.0 35.0 108-109 29.55836052985218 35.0 33.0 35.0 2.0 35.0 110-111 29.48552413862454 35.0 33.0 35.0 2.0 35.0 112-113 29.392638885079855 35.0 33.0 35.0 2.0 35.0 114-115 29.27908199179077 35.0 33.0 35.0 2.0 35.0 116-117 29.15795006810557 35.0 33.0 35.0 2.0 35.0 118-119 29.049375394235252 35.0 33.0 35.0 2.0 35.0 120-121 28.896172147108025 35.0 32.0 35.0 2.0 35.0 122-123 28.703756776275494 35.0 32.0 35.0 2.0 35.0 124-125 27.107532521551526 33.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2553895224758591 1101 2 0.24927097317578983 1101 3 0.17855404846708467 1101 4 0.022814453354975228 1101 5 0.04805430108203268 1101 6 0.24505628015985792 1101 7 0.14101753827126373 1101 8 0.07977927664568796 1101 9 0.1547929873449192 1101 10-11 0.18356218042126926 1101 12-13 0.2353967559579928 1101 14-15 0.32615254113083125 1101 16-17 0.19272753866690806 1101 18-19 0.13755265515896298 1101 20-21 0.10706239887966973 1101 22-23 0.19796322760282692 1101 24-25 0.20543770275516238 1101 26-27 0.1960013089316206 1101 28-29 0.1458087316716643 1101 30-31 0.10888036372320187 1101 32-33 0.26371438395636915 1101 34-35 0.24779664212456964 1101 36-37 0.2634370838506115 1101 38-39 0.14885556435717717 1101 40-41 0.21316633423860054 1101 42-43 0.0419585965133038 1101 44-45 0.23159577740151605 1101 46-47 0.11501699286615974 1101 48-49 0.19225175997208765 1101 50-51 0.5180524864789007 1101 52-53 0.40331927235222054 1101 54-55 0.4717854144858862 1101 56-57 0.43362400495617237 1101 58-59 0.40584981498304273 1101 60-61 0.5226788088036898 1101 62-63 0.6050995735988636 1101 64-65 0.5000993345480609 1101 66-67 0.6848901245696339 1101 68-69 0.48670126162662086 1101 70-71 0.623019750629858 1101 72-73 0.522274879300376 1101 74-75 0.6456592606505609 1101 76-77 0.6204631778573528 1101 78-79 0.7931745019521124 1101 80-81 0.7192276615442132 1101 82-83 0.6415345296856323 1101 84-85 0.5166959212157032 1101 86-87 0.5820582293930379 1101 88-89 0.43946038576195434 1101 90-91 0.48353886060660045 1101 92-93 0.44531265447752943 1101 94-95 0.3666499564856309 1101 96-97 0.4633764240734841 1101 98-99 0.48969318819198904 1101 100-101 0.4556095005864158 1101 102-103 0.3242785576924305 1101 104-105 0.3330120941414414 1101 106-107 0.4130433555007684 1101 108-109 0.46106026533568567 1101 110-111 0.3986776481337948 1101 112-113 0.3906379881892832 1101 114-115 0.47491580792614485 1101 116-117 0.44595588559640476 1101 118-119 0.6050294745348843 1101 120-121 0.598670743411958 1101 122-123 0.739780466896395 1101 124-125 0.6227332116258353 1102 1 0.11482090758934049 1102 2 0.1386985360460713 1102 3 0.09644327086542859 1102 4 -0.09997792014564055 1102 5 -0.1384093370189916 1102 6 -0.008869950546248617 1102 7 -0.018898372378181705 1102 8 -0.036473126308685266 1102 9 -0.011497075946934388 1102 10-11 -0.007046905275664983 1102 12-13 0.06730658474398155 1102 14-15 -0.02950578405212667 1102 16-17 0.08184875998603758 1102 18-19 0.06422320027829898 1102 20-21 0.1586078779528961 1102 22-23 0.20173628753335038 1102 24-25 0.1188231422569217 1102 26-27 0.12226152500078058 1102 28-29 0.06320331083144026 1102 30-31 -0.04844148039672547 1102 32-33 -0.25663625064842677 1102 34-35 -0.143092366677827 1102 36-37 -0.12377863681086154 1102 38-39 -0.015808640335187363 1102 40-41 -0.1187701940403798 1102 42-43 -0.07804016687859416 1102 44-45 -0.2694216138050791 1102 46-47 -0.756540177774518 1102 48-49 -0.6005025597248306 1102 50-51 -0.4874700928607538 1102 52-53 -0.4326811998072344 1102 54-55 -0.3849732108555841 1102 56-57 -0.4948761580512624 1102 58-59 -0.4133932122335864 1102 60-61 -0.31645674592746786 1102 62-63 -0.18435384562252466 1102 64-65 -0.40590720823295356 1102 66-67 -0.4721829926945418 1102 68-69 -0.4416710248617548 1102 70-71 -0.18859565301026038 1102 72-73 -0.17735863502742433 1102 74-75 -0.23203176719560759 1102 76-77 -0.17703240970012146 1102 78-79 -0.03588691250271481 1102 80-81 -0.19963820020300105 1102 82-83 -0.323593121995593 1102 84-85 -0.3640360383584529 1102 86-87 -0.33026344609850966 1102 88-89 -0.26209970777058444 1102 90-91 -0.2178708726233083 1102 92-93 -0.41491690495273303 1102 94-95 -0.4053096738809643 1102 96-97 -0.4777610305373763 1102 98-99 -0.40530192158508527 1102 100-101 -0.6820994580771504 1102 102-103 -0.6587518066572855 1102 104-105 -0.5253147751041176 1102 106-107 -0.5886752487059468 1102 108-109 -0.6545963173540486 1102 110-111 -0.5785476843451569 1102 112-113 -0.44154024076308573 1102 114-115 -0.5121658531757838 1102 116-117 -0.49563264992235645 1102 118-119 -0.49573019630252446 1102 120-121 -0.7145550612264451 1102 122-123 -0.6512546864992217 1102 124-125 -0.34667338511626866 1103 1 0.008252411458435915 1103 2 -0.034120852350355335 1103 3 -0.13529834610453406 1103 4 -0.061821660636432796 1103 5 0.04505182845751676 1103 6 -0.04160294204590542 1103 7 -0.01468943863191896 1103 8 0.03872459404179551 1103 9 0.03242656034431235 1103 10-11 -0.08204839830960253 1103 12-13 0.008654524214961157 1103 14-15 -0.07572158291059594 1103 16-17 -0.029068019724626026 1103 18-19 -0.08665143321741908 1103 20-21 -0.023512400860532523 1103 22-23 0.1185615001840361 1103 24-25 0.11708837902367009 1103 26-27 0.003571015293218238 1103 28-29 0.029014255765503094 1103 30-31 -0.0932452957382921 1103 32-33 -0.2132884988731405 1103 34-35 -0.1129416129089833 1103 36-37 -0.09704764171085145 1103 38-39 -0.0453181004825538 1103 40-41 0.045361158818998604 1103 42-43 -0.02344915863989172 1103 44-45 0.2710723102578001 1103 46-47 0.3021772918600121 1103 48-49 -0.07613482404587302 1103 50-51 -0.3570258512793316 1103 52-53 -0.15755343876091388 1103 54-55 -0.27796960197928655 1103 56-57 0.032439253187163786 1103 58-59 0.013857643061570002 1103 60-61 0.03158794839872314 1103 62-63 -0.15190239766399571 1103 64-65 -0.1506623933265061 1103 66-67 -0.04871164614856838 1103 68-69 0.17459223825633075 1103 70-71 0.019014269835430753 1103 72-73 -0.16572434586797868 1103 74-75 -0.15591831936789902 1103 76-77 -0.10070140549971995 1103 78-79 -0.07924035202630719 1103 80-81 -0.05506701376714318 1103 82-83 -0.10928303164570963 1103 84-85 -0.10965576119447817 1103 86-87 -0.10284451784092496 1103 88-89 -0.12536197268883953 1103 90-91 -0.1468664447112502 1103 92-93 -0.2076596225019962 1103 94-95 -0.13925955913064314 1103 96-97 -0.04424085467219996 1103 98-99 -0.06602062546100385 1103 100-101 -0.23456751426072842 1103 102-103 -0.24688631052922005 1103 104-105 -0.4972969269499323 1103 106-107 -0.25727574979656964 1103 108-109 -0.20391620485038686 1103 110-111 -0.29516842244425234 1103 112-113 -0.18037590180741248 1103 114-115 -0.1379632043913972 1103 116-117 -0.055065353906336156 1103 118-119 0.07968934623187707 1103 120-121 0.123238888715683 1103 122-123 -0.0010753802338783203 1103 124-125 0.04064521126120724 1104 1 0.05296823587062249 1104 2 0.08961603888717917 1104 3 0.11501146015054786 1104 4 0.01480284326412118 1104 5 -0.05851899570900798 1104 6 0.06106439427539101 1104 7 0.042601517209092776 1104 8 0.14172493796768038 1104 9 0.15776025034153207 1104 10-11 0.3143753183828224 1104 12-13 0.29874782093565955 1104 14-15 0.3445954294106883 1104 16-17 0.23603878837018755 1104 18-19 0.26340506889253845 1104 20-21 0.15865231234349864 1104 22-23 0.15305235339319267 1104 24-25 0.2634261142444103 1104 26-27 0.3003599485224697 1104 28-29 0.3842310109612441 1104 30-31 0.2078358871483985 1104 32-33 0.18432121621429332 1104 34-35 0.4626210880556485 1104 36-37 0.32073223480337276 1104 38-39 0.587090636326792 1104 40-41 0.5245509131030488 1104 42-43 0.38099267148255933 1104 44-45 0.4827341273874879 1104 46-47 0.4995661846916164 1104 48-49 0.6003987851560524 1104 50-51 0.7910083120254114 1104 52-53 0.686439322418039 1104 54-55 0.6106802978454837 1104 56-57 0.6431424451469354 1104 58-59 0.5557046888473849 1104 60-61 0.4791706407379053 1104 62-63 0.4356526714214297 1104 64-65 0.5707849341509856 1104 66-67 0.5874802754144923 1104 68-69 0.41243336633323935 1104 70-71 0.39569773245941775 1104 72-73 0.40216007319752833 1104 74-75 0.40366547571778 1104 76-77 0.48263920668836136 1104 78-79 0.5115165032900784 1104 80-81 0.5252792595502136 1104 82-83 0.459522617473457 1104 84-85 0.6811308464406771 1104 86-87 0.7467575107758933 1104 88-89 0.7551906561605399 1104 90-91 0.7483589905187671 1104 92-93 0.5956611730040393 1104 94-95 0.681991785182845 1104 96-97 0.8872742207458337 1104 98-99 0.7523714937229649 1104 100-101 0.8687631076992126 1104 102-103 0.8779806229074723 1104 104-105 0.7510994503640624 1104 106-107 0.77369259264443 1104 108-109 0.8811222002069634 1104 110-111 0.6953149299050878 1104 112-113 0.7377915737219887 1104 114-115 0.7370033583696163 1104 116-117 0.6640632034064566 1104 118-119 0.6741747948913854 1104 120-121 0.808653155634925 1104 122-123 0.6969385842489473 1104 124-125 0.8709177861742674 1105 1 -0.04541562696559254 1105 2 -0.10944617002657253 1105 3 0.009483650066400173 1105 4 -0.11729308297561403 1105 5 -0.23145777615959418 1105 6 -0.14432079999260594 1105 7 -0.09964854988875516 1105 8 -0.025032946687169044 1105 9 -0.013991819166577102 1105 10-11 0.07324407499393004 1105 12-13 0.13679203710124455 1105 14-15 -0.01741587460308125 1105 16-17 0.043923180095312375 1105 18-19 0.12418887699795533 1105 20-21 0.1252125872604779 1105 22-23 0.08304956422778531 1105 24-25 0.11029303947898939 1105 26-27 0.14798205344771276 1105 28-29 -0.09024944119930467 1105 30-31 -0.26725239256806077 1105 32-33 -0.0836504816506789 1105 34-35 -0.23569120374368424 1105 36-37 -0.04264602338913903 1105 38-39 -0.13767136366268318 1105 40-41 -0.1755269730253204 1105 42-43 -0.18977450688193898 1105 44-45 -0.4027916829176874 1105 46-47 -0.31672517257941735 1105 48-49 0.06113075688549685 1105 50-51 0.2562608367463639 1105 52-53 0.24802593652911753 1105 54-55 0.32894827875285415 1105 56-57 0.312152712433182 1105 58-59 0.2040146124873985 1105 60-61 0.31898176687660396 1105 62-63 0.5190842395112725 1105 64-65 0.3727857656380351 1105 66-67 0.19128985435576595 1105 68-69 0.16988648993537225 1105 70-71 0.15900020955405836 1105 72-73 0.1970406390297157 1105 74-75 0.17966391799521375 1105 76-77 -0.039230744527557704 1105 78-79 0.05791269107984931 1105 80-81 0.1407599169512359 1105 82-83 0.21400896371939737 1105 84-85 0.06014158735878894 1105 86-87 0.06267917206595897 1105 88-89 -0.031849986715773326 1105 90-91 -0.010117704162347252 1105 92-93 -0.1354659129496163 1105 94-95 0.0059322970210082815 1105 96-97 0.0535795238439114 1105 98-99 0.05731805226123399 1105 100-101 -0.04141787229773186 1105 102-103 -0.07512719149198688 1105 104-105 0.008434166000753862 1105 106-107 -0.05977186654692446 1105 108-109 -0.1276544613199242 1105 110-111 -0.02366744572708157 1105 112-113 0.01886050820817431 1105 114-115 0.015314702905222077 1105 116-117 0.05818937893442211 1105 118-119 0.1299020566150908 1105 120-121 0.06931381945629056 1105 122-123 -0.0044417894992179185 1105 124-125 0.04411443286068817 1106 1 0.12624901587269477 1106 2 0.09720342608767396 1106 3 0.1148714331823868 1106 4 0.14963844123114 1106 5 0.15345590316039903 1106 6 0.0898062260355843 1106 7 0.15992855797249916 1106 8 -0.021292383886894584 1106 9 0.08285100856163297 1106 10-11 0.02461543870228411 1106 12-13 0.05653228312837655 1106 14-15 0.1102291810961944 1106 16-17 0.12390311779659413 1106 18-19 -0.03368029569619324 1106 20-21 0.12648759913947316 1106 22-23 0.15693161902953534 1106 24-25 0.15944831111195157 1106 26-27 0.17093094738150683 1106 28-29 0.2448886190931816 1106 30-31 0.24338458541620867 1106 32-33 0.3684177829604778 1106 34-35 0.714477741929727 1106 36-37 0.6246841070158027 1106 38-39 0.41932536301489876 1106 40-41 0.39625250007535584 1106 42-43 0.17769685154686954 1106 44-45 0.033084194807877054 1106 46-47 -0.01913000864932002 1106 48-49 0.046148707363293795 1106 50-51 0.2033306852232144 1106 52-53 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2216 98-99 -0.17100013521050883 2216 100-101 -0.17280287463723099 2216 102-103 -0.08525909177608071 2216 104-105 -0.060987711559615576 2216 106-107 0.00525136061730791 2216 108-109 -0.006305607673759539 2216 110-111 0.04461133552298335 2216 112-113 0.050078488185235415 2216 114-115 -0.1704957010480399 2216 116-117 -0.1480210494996541 2216 118-119 -0.07081005882381675 2216 120-121 -0.2903794990569324 2216 122-123 -0.34692746131014474 2216 124-125 -0.654621448715325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 10.0 5 45.0 6 143.0 7 298.0 8 624.0 9 1184.0 10 2408.0 11 3162.0 12 3449.0 13 5539.0 14 10162.0 15 10536.0 16 3309.0 17 2432.0 18 2223.0 19 2392.0 20 2231.0 21 2206.0 22 2175.0 23 1634.0 24 1802.0 25 2159.0 26 2454.0 27 2888.0 28 3399.0 29 4172.0 30 5228.0 31 6463.0 32 8055.0 33 10674.0 34 15454.0 35 28044.0 36 49514.0 37 142153.0 38 98624.0 39 307.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17796005706134 21.124355316580708 15.18869563627053 19.50898899008742 2 14.967740166064436 23.172456981174335 41.49713969662272 20.36266315613851 3 15.923738652673535 32.90701820143161 32.20355252452302 18.965690621371827 4 10.23371347798043 19.65567403625784 40.43988819813267 29.670724287629056 5 9.73568432961181 40.872367413637456 34.83138877144065 14.560559485310081 6 24.376444447492403 42.34165655124802 18.37645816331429 14.905440837945278 7 22.96437484573403 37.214208009946155 24.175191289800622 15.646225854519194 8 25.582096920165647 33.92297214527969 21.629231458373326 18.865699476181337 9 28.285750852462314 14.581447860388156 22.02552359012332 35.107277697026205 10-11 22.465467277331356 29.182664618928776 27.915855341944802 20.436012761795062 12-13 25.635690060243714 27.89654352814268 28.923612063370175 17.544154348243424 14-15 22.931693314684292 26.644818034720124 30.165053158909945 20.258435491685635 16-17 17.875197688981526 30.52832094634744 30.59448390606002 21.00199745861101 18-19 18.204641234493412 25.89805647734233 37.421610033915655 18.4756922542486 20-21 19.50047536772436 25.120670268491345 38.043587563649 17.335266800135297 22-23 19.146806351644134 25.288534496155922 37.823729991132566 17.740929161067385 24-25 18.585591721250406 25.83692527093799 38.168774596964035 17.408708410847577 26-27 17.9270767627458 25.819552240170403 38.258988563749554 17.99438243333425 28-29 17.983515158717733 24.384789517167615 39.002544831660586 18.629150492454066 30-31 18.44228151794928 25.180075630050432 38.34230997349863 18.035332878501663 32-33 18.12499428644562 25.556157383283512 37.843841702547785 18.475006627723083 34-35 18.42278474069605 25.212887036173655 38.283556847580655 18.080771375549645 36-37 18.766390852775718 24.88107168609096 37.05626854773188 19.296268913401445 38-39 18.469864428781687 25.244654398522705 37.230320233295856 19.055160939399755 40-41 18.376162136960755 25.101815539039574 37.26345884869594 19.258563475303735 42-43 18.67829489253947 24.84173454369269 36.81814442037133 19.661826143396503 44-45 18.677798715761348 25.21738236097414 36.03021926220844 20.074599661056077 46-47 19.703526620781705 25.456897428484254 34.37052544489862 20.46905050583542 48-49 19.358664948029507 26.38233734653393 32.89441808591357 21.364579619522985 50-51 19.74229113148729 26.325568851874536 32.293371241555214 21.638768775082962 52-53 19.649027781586813 26.752804212489373 32.23050306703599 21.367664938887824 54-55 19.585210274553365 26.967388646248292 31.90640091235913 21.54100016683922 56-57 19.80162493857915 27.075339100227403 31.25127126875479 21.871764692438664 58-59 19.454846513456285 26.2984619920397 32.313594097998724 21.933097396505286 60-61 20.050576383365787 27.36861110349304 31.496425600380295 21.08438691276088 62-63 20.33926389972928 26.990038522588456 32.28069493789647 20.390002639785802 64-65 20.32309893405006 26.48659334832611 32.40416963779643 20.786138079827403 66-67 20.059832341460293 27.00191518342795 31.670231924599367 21.268020550512393 68-69 20.473273248565587 26.9003321917456 31.822072295985475 20.804322263703337 70-71 20.51617842838124 26.60724386773856 31.99174937291806 20.884828330962137 72-73 20.18670164989498 27.075396018219923 31.127340627092654 21.610561704792445 74-75 19.724903783744253 27.46722705208019 31.230516779566504 21.577352384609057 76-77 19.27861464025579 27.04555167787689 32.45628469627263 21.21954898559469 78-79 19.33139069820592 26.827676836932923 31.974974288652724 21.865958176208434 80-81 19.163876541234817 27.502142587788963 31.32097679148907 22.01300407948715 82-83 19.947046172903274 26.623296484295544 31.168138114185545 22.261519228615636 84-85 19.314327766045945 26.547116254833668 31.200463483531248 22.938092495589135 86-87 18.830949186708505 27.11093538235099 31.20070484500952 22.85741058593098 88-89 19.36821631900242 26.34850836154034 31.46576648024519 22.817508839212046 90-91 19.346474392305062 26.564076661383275 31.408494644422326 22.680954301889336 92-93 19.374811442363075 26.65862154082444 31.180690783759818 22.78587623305267 94-95 19.009233441513917 26.042418978836217 31.648992092151573 23.299355487498286 96-97 19.37274306349134 26.156237144032545 31.35107647300818 23.119943319467932 98-99 18.99525292949332 26.38600974097954 31.38094726569196 23.237790063835185 100-101 19.048326474826556 26.165593798889002 31.417293950534187 23.368785775750254 102-103 19.67646793304925 25.800631020088193 31.11413555847335 23.408765488389207 104-105 18.957218701362812 26.32058891420657 31.182764980539414 23.539427403891203 106-107 19.255728701844678 25.912941329728454 31.17490238381188 23.65642758461498 108-109 19.312791198644184 26.174479770800886 30.230490476511545 24.282238554043385 110-111 19.10540816338191 26.477226109383693 30.342256854336004 24.07510887289839 112-113 18.715896170086335 26.45057337287235 30.92522440677191 23.908306050269402 114-115 18.66263975354012 26.78754262311567 30.366284544149778 24.183533079194437 116-117 18.37894664670727 26.670475711624825 30.53353292728914 24.417044714378765 118-119 17.913601323725352 26.599063427744753 30.45322509501706 25.034110153512835 120-121 17.968639299377905 26.731443433267664 30.320029070697558 24.97988819665687 122-123 17.962694421304153 27.043630591808682 30.221449736429218 24.77222525045795 124-125 17.73056455807626 27.01464981623426 29.902429823207644 25.352355802481835 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1201.0 1 804.0 2 732.0 3 931.5 4 496.5 5 320.5 6 776.5 7 1872.5 8 3103.0 9 3302.0 10 2131.0 11 1055.0 12 861.0 13 843.0 14 871.0 15 905.0 16 983.0 17 1097.5 18 1196.0 19 1256.0 20 1366.5 21 1469.5 22 1565.0 23 1789.0 24 1948.5 25 2255.5 26 2652.0 27 2980.5 28 3346.0 29 4039.5 30 5310.5 31 6262.5 32 8100.0 33 11000.0 34 13459.0 35 15248.0 36 18937.0 37 21883.5 38 22092.5 39 22654.5 40 21054.5 41 18495.5 42 16593.5 43 15675.0 44 15503.0 45 15592.0 46 15005.0 47 13366.5 48 11895.0 49 10654.0 50 9870.0 51 8669.5 52 7868.0 53 7647.0 54 7457.5 55 7025.5 56 6368.0 57 5586.5 58 5101.5 59 4898.0 60 4274.5 61 3921.5 62 3538.5 63 2353.0 64 1415.0 65 1175.5 66 946.5 67 664.5 68 502.5 69 365.0 70 273.0 71 245.0 72 222.0 73 253.5 74 206.0 75 118.0 76 81.0 77 59.0 78 43.0 79 18.5 80 17.0 81 23.5 82 14.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030167567122836848 2 0.0034281326275950965 3 0.0018283374013840516 4 0.08090393001124427 5 0.002513963926903071 6 0.023996928393165674 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.570843503460129E-4 26-27 0.0 28-29 5.71355437932516E-4 30-31 0.007199078517949703 32-33 0.0 34-35 0.0 36-37 5.71355437932516E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0046851145910466315 46-47 0.0028567771896625804 48-49 0.0 50-51 0.0018283374013840516 52-53 0.0 54-55 0.002056879576557058 56-57 2.2854217517300645E-4 58-59 0.002171150664143561 60-61 0.0 62-63 0.0046851145910466315 64-65 0.002742506102076077 66-67 0.0 68-69 0.0017140663137975483 70-71 0.0030853193648355867 72-73 0.0029710482772490835 74-75 0.0 76-77 0.002056879576557058 78-79 0.0013712530510380386 80-81 2.2854217517300645E-4 82-83 0.001142710875865032 84-85 0.0 86-87 0.0029710482772490835 88-89 0.003885216977941109 90-91 0.004913656766219638 92-93 0.005485012204152154 94-95 0.0036566748027681032 96-97 0.0036566748027681032 98-99 0.005256470028979148 100-101 0.004913656766219638 102-103 0.0023996928393165673 104-105 0.001142710875865032 106-107 1.1427108758650323E-4 108-109 0.007427620693122708 110-111 0.001028439788278529 112-113 0.0030853193648355867 114-115 0.0 116-117 2.2854217517300645E-4 118-119 6.856265255190193E-4 120-121 4.570843503460129E-4 122-123 0.0017140663137975483 124-125 0.04079477826838165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 437556.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.534165625654513 #Duplication Level Percentage of deduplicated Percentage of total 1 66.7078040344199 19.034515288418415 2 12.591605444686916 7.185819105031791 3 5.862988452410074 5.018864506871067 4 3.3730422819300645 3.849877885397124 5 2.1869843817177097 3.120188728432638 6 1.5093263462048494 2.5840420767463885 7 1.1033504056454975 2.20382282524752 8 0.8412513422901333 1.9203524106968677 9 0.6962162548722778 1.787935493501862 >10 4.3719364735979145 24.078922258466065 >50 0.4582639459517721 9.149439256762891 >100 0.2844120643581802 14.075409751346674 >500 0.0072104467020383724 1.2998453792557727 >1k 0.004005803723354651 1.8871304266191355 >5k 0.0016023214893418603 2.803834607205778 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6873 1.5707703699640732 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5392 1.2322994085328507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3626 0.8286939271773214 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1313 0.30007587600215746 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1234 0.28202104416348994 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1067 0.24385450090959787 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1015 0.23197030780060152 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 914 0.20888754810812785 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 911 0.20820192158260883 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 661 0.15106637778935725 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 557 0.12729799157136457 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 556 0.12706944939619158 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 531 0.12135589501686642 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 526 0.12021318414100139 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 522 0.11929901544030935 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 508 0.11609942498788726 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 485 0.11084295495890813 TruSeq Adapter, Index 11 (95% over 21bp) GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 482 0.1101573284333891 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 459 0.10490085840440995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 453 0.10352960535337191 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 451 0.1030725210030259 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 448 0.10238689447750687 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 448 0.10238689447750687 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 445 0.10170126795198786 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 443 0.10124418360164184 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 442 0.10101564142646885 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2854217517300643E-4 0.0 16-17 0.0 0.0 0.0 2.2854217517300643E-4 0.0 18-19 0.0 0.0 0.0 5.71355437932516E-4 0.0 20-21 0.0 0.0 0.0 0.0013712530510380386 0.0 22-23 0.0 0.0 0.0 0.004342301328287122 0.0 24-25 0.0 0.0 0.0 0.009141687006920257 0.0 26-27 0.0 0.0 0.0 0.014512428123485908 0.0 28-29 0.0 0.0 0.0 0.02548245253179022 0.0 30-31 0.0 0.0 0.0 0.05987804989532768 0.0 32-33 0.0 0.0 0.0 0.10924315973269708 0.0 34-35 0.0 0.0 0.0 0.16146504675972903 0.0 36-37 0.0 0.0 0.0 0.24625419374891444 0.0 38-39 0.0 0.0 0.0 0.38920732431962995 0.0 40-41 0.0 0.0 0.0 0.5647277148524988 0.0 42-43 0.0 0.0 0.0 0.7201363939701433 0.0 44-45 0.0 0.0 0.0 0.8868579107588515 0.0 46-47 0.0 0.0 0.0 1.0821471994441854 0.0 48-49 0.0 0.0 0.0 1.296634030844052 0.0 50-51 0.0 0.0 0.0 1.5532868935633384 0.0 52-53 0.0 0.0 0.0 1.7885710629039484 0.0 54-55 0.0 0.0 0.0 2.007857279982448 0.0 56-57 0.0 0.0 0.0 2.2525116785051513 0.0 58-59 0.0 0.0 0.0 2.522191445209299 0.0 60-61 0.0 0.0 0.0 2.8595196957646563 0.0 62-63 0.0 0.0 0.0 3.1971907595827735 0.0 64-65 0.0 0.0 0.0 3.500923310387699 0.0 66-67 0.0 0.0 0.0 3.8143689036374773 0.0 68-69 0.0 0.0 0.0 4.1931775589867355 0.0 70-71 0.0 0.0 0.0 4.625007998976131 0.0 72-73 0.0 0.0 0.0 5.069522529687628 0.0 74-75 0.0 0.0 0.0 5.493239722458382 0.0 76-77 0.0 0.0 0.0 5.925870060060884 0.0 78-79 0.0 0.0 0.0 6.41552167036905 0.0 80-81 0.0 0.0 0.0 6.984248873287076 0.0 82-83 0.0 0.0 0.0 7.5821152035396615 0.0 84-85 0.0 0.0 0.0 8.174610792675681 0.0 86-87 0.0 0.0 0.0 8.777162237519313 0.0 88-89 0.0 0.0 0.0 9.394226110486429 0.0 90-91 0.0 0.0 0.0 10.10624925723793 0.0 92-93 0.0 0.0 0.0 10.900661858139301 0.0 94-95 0.0 0.0 0.0 11.688675278135827 0.0 96-97 0.0 0.0 0.0 12.450520619075045 0.0 98-99 0.0 0.0 0.0 13.244590406713655 0.0 100-101 0.0 0.0 0.0 14.120935377414547 0.0 102-103 0.0 0.0 0.0 15.078412820301859 0.0 104-105 0.0 0.0 0.0 16.040004022342284 0.0 106-107 0.0 0.0 0.0 16.99165363976268 0.0 108-109 0.0 0.0 0.0 17.921820292716816 0.0 110-111 0.0 0.0 0.0 18.95734488842571 0.0 112-113 0.0 0.0 0.0 20.03960635895748 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2065 0.0 96.91438 1 GTATCAA 4600 0.0 61.89265 1 TGGGTAT 80 3.832247E-8 52.07501 6 AGGCTCG 35 0.0077856467 51.01225 5 ATCAACG 6155 0.0 45.858505 3 TCAACGC 6225 0.0 45.34283 4 CAACGCA 6335 0.0 44.43609 5 TATCAAC 6415 0.0 43.999863 2 AACGCAG 6460 0.0 43.576256 6 ACGCAGA 7300 0.0 38.377007 7 CGCAGAG 7325 0.0 38.164825 8 GTTATAT 80 1.1889491E-4 37.22623 1 TGGTATC 710 0.0 36.903194 2 GTACATG 3535 0.0 35.72033 1 GCAGAGT 7780 0.0 35.39765 9 GTATGGT 170 3.2196112E-10 35.03645 1 CATATAG 140 7.9890015E-8 34.02762 3 GATAGTC 105 1.48495765E-5 33.98874 7 ACATGGG 3625 0.0 33.511337 3 ATGGGAT 1145 0.0 32.745853 5 >>END_MODULE