FastQCFastQC Report
Fri 27 May 2016
SRR940112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791359
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.053.8947751
ATCAACG38650.045.272393
TCAACGC39850.043.7597544
AACGCAG41900.041.894666
TATCAAC43000.041.5229642
CAACGCA42500.041.171245
GTACATG78850.037.966611
TACATGG84250.035.2505762
ACATGGG84000.034.3635523
ACGCAGA51600.033.7818767
CGCAGAG52850.032.9856348
CATGGGG50300.029.6989634
GGTATCA37500.029.2026421
GTGTACG3100.026.8783051
GCAGAGT63150.026.19239
CATGGGA42050.026.0427844
TAAGGTG10200.023.9231995
GAGTACA61300.023.4958131
GTAAGGT9200.023.2889674
AGAGTAC54200.021.90008410-11
Produced by FastQC (version 0.11.5)