FastQCFastQC Report
Fri 27 May 2016
SRR940112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791359
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38540.21514392145851277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31150.17389032572477098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58200.057.944441
GGTATCA47350.049.578641
ATCAACG76300.043.4057463
TCAACGC77700.042.700324
TATCAAC79850.041.5517542
CAACGCA80150.041.427715
AACGCAG80700.041.1453676
GTGGTAT13150.037.6071781
TGGTATC13450.035.4304662
ACGCAGA94850.034.8111957
TATACCG1408.0628524E-834.003395
CGCAGAG97850.033.6223148
GTACATG77750.032.722451
TCCGTTG2900.030.7720578
TACATGG81600.030.7327232
ACATGGG80950.030.3899633
GCAGAGT109600.029.5291969
TATTCCG3450.029.3217645
GAGTACT65550.025.82098812-13
AGAGTAC103050.025.71952810-11
Produced by FastQC (version 0.11.5)