FastQCFastQC Report
Fri 27 May 2016
SRR940111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041619
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20960.20122520806552108No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG12940.12422968475037419No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA12180.11693335087013582No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA10860.10426077097287971No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA10510.10090061721224364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.076.219121
GTATCAA26350.061.904341
ATCAACG36000.042.8300323
TCAACGC36300.042.4760674
CAACGCA37000.041.833365
GTACATG73750.041.5715641
AACGCAG37400.040.8887756
TATCAAC40400.039.933652
TACATGG76600.038.703632
ACATGGG77150.036.498613
ACGCAGA46250.033.0567137
CGCAGAG45600.032.748318
CATGGGG29100.031.7095344
CATGGGA51900.031.6586844
TAGCCCG800.0053478629.7660455
TATAGGG1602.5902955E-729.7660452
TTACCGG400.00536731429.74532324-25
ATGGGAG19450.028.7712945
GAGTACA53750.028.1323741
GCAGAGT51100.027.5935429
Produced by FastQC (version 0.11.5)