FastQCFastQC Report
Fri 27 May 2016
SRR940111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041619
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70980.6814391826569984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56070.538296632453901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.3426396791917198No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG11960.11482125422059314No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA11550.11088507410099085No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA10890.1045487841523628No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.10368474461391354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28150.087.2063751
GTATCAA61800.064.404681
ATCAACG82800.047.7057653
TATCAAC83700.047.335162
TCAACGC84000.047.024254
CAACGCA85250.046.418735
AACGCAG87200.045.5149966
GTGGTAT7000.045.1135751
ACGCAGA101150.039.053867
CGCAGAG102300.038.6148388
GCAGAGT109500.035.2064869
GTACATG71300.034.5135731
TGGTATC9500.033.8655242
ATGGGAG18650.031.907095
ACATGGG73850.031.6244683
TACATGG77000.031.4913522
GAGTACT67600.029.52620312-13
TAAGGTA4050.029.4213524
AGGTAAG4700.029.155312
AGAGTAC104850.029.05114710-11
Produced by FastQC (version 0.11.5)