FastQCFastQC Report
Fri 27 May 2016
SRR940110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452329
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26970.10997708708741771No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25870.10549155517061537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.064.3940351
GTATCAA44200.053.8706321
TCAACGC51900.045.3038564
ATCAACG54300.043.4111023
CAACGCA55550.042.433395
TATCAAC56650.041.9255182
AACGCAG56250.041.6817786
GTACATG112400.037.86641
TACATGG119350.034.9125062
ACGCAGA68050.034.445657
ACATGGG117750.034.42643
CGCAGAG71650.032.1378
CATGGGG72500.029.8039654
GTATAGG8550.025.760241
GCAGAGT86500.025.5880629
CATGGGA55350.025.4880244
ATGGGGG41050.024.7958815
GAGTACA87000.023.1265981
GTGTAAG14000.022.5352381
CAGAGTA77900.022.30216810-11
Produced by FastQC (version 0.11.5)