FastQCFastQC Report
Fri 27 May 2016
SRR940110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452329
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41490.1691861083892088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32640.13309796524038983No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26280.10716343524869625No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25720.10487989172741503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35250.072.333451
GTATCAA69850.059.4457631
TCAACGC88150.046.687854
ATCAACG89600.045.933233
TATCAAC91650.045.1657642
CAACGCA92100.044.648135
AACGCAG93750.043.9257976
ACGCAGA111300.036.882787
GTACATG103150.036.789571
CGCAGAG115000.035.5926488
ACATGGG107400.033.273923
TACATGG112750.033.0685232
GCAGAGT129800.030.8926339
CCGTACG800.005366228529.7467659
CATGGGG65950.029.350584
GTGGTAT20600.027.4733071
GAGTACT71800.026.80523112-13
TGGTATC20750.025.833392
CAGAGTA124550.025.60299510-11
AGAGTAC119450.025.3264610-11
Produced by FastQC (version 0.11.5)