FastQCFastQC Report
Fri 27 May 2016
SRR940109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139441
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.057.3852651
GTATCAA35850.053.6265871
TCAACGC43250.044.3125154
ATCAACG43550.044.0072633
CAACGCA44700.043.0082365
AACGCAG45300.042.4266856
TATCAAC49350.039.0763972
GTACATG104750.037.2749061
TACATGG107100.035.7337152
ACATGGG110000.033.9259073
ACGCAGA57600.033.1555637
CGCAGAG60000.031.534828
CATGGGG59500.030.8099084
TAAGGTG8650.026.1471355
GCAGAGT71500.025.6306239
CATGGGA63300.025.105234
GTAAGGT7600.024.2775444
ATGGGAG30800.023.962255
GAGTACA79700.023.6737861
AGTACAT78250.022.4385242
Produced by FastQC (version 0.11.5)