FastQCFastQC Report
Fri 27 May 2016
SRR940109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139441
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34340.16050921712727764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.11956394216994067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.071.66341
GTATCAA59950.060.5350271
ATCAACG76300.046.5337643
TCAACGC76400.046.4706844
TATCAAC79850.044.8379822
CAACGCA79150.044.8110125
AACGCAG81150.043.7778976
ACGCAGA97400.036.4053347
GTACATG103500.036.2726141
TACATGG104150.034.8912932
CGCAGAG101650.034.7076388
ACATGGG107600.032.7759973
GCAGAGT111150.031.0453289
CATGGGG58300.029.5294574
CCTATCG900.00953495926.4420558
CATGGGA58950.025.3639034
GAGTACT60450.025.34303712-13
CAGAGTA110150.024.81868210-11
AGAGTAC109100.024.4576910-11
GAGTACA79350.024.4070381
Produced by FastQC (version 0.11.5)