FastQCFastQC Report
Fri 27 May 2016
SRR940108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370936
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29010.12235674012288818No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27860.11750633505079851No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25500.10755246029416231No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46850.058.1918031
GGTATCA33250.047.6207121
TCAACGC57600.046.7112924
ATCAACG57450.046.6260263
CAACGCA58700.045.937365
AACGCAG61550.043.599386
TATCAAC65500.041.4409262
GTACATG136750.038.1313931
TACATGG147250.035.1293752
ACATGGG143100.034.9418263
ACGCAGA77200.034.6787077
CGCAGAG78150.033.880098
CATGGGA79850.031.6079884
CATGGGG65150.029.6944224
GTAAGGT10650.029.0642854
CCGTTGC3800.028.184589
GCAGAGT92150.027.8940169
TAAGGTG12300.027.1012215
ATGGGAG36850.026.4918485
TATTCCG5100.024.5106625
Produced by FastQC (version 0.11.5)