FastQCFastQC Report
Fri 27 May 2016
SRR940108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370936
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42790.18047724611714527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32930.13889029480340254No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28620.12071182014191863No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27210.11476480174918259No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26980.11379472073476467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.064.441081
GTATCAA74150.061.6224481
ATCAACG96200.047.1134723
TCAACGC96650.046.893124
CAACGCA99750.045.3993725
TATCAAC102250.044.619892
AACGCAG103150.043.9020046
GTACATG129200.040.023211
ACGCAGA120350.037.5711067
ACATGGG135550.037.0393643
CGCAGAG122100.036.935168
TACATGG140800.036.548882
CATGGGA77250.032.225834
GCAGAGT137100.032.02629
CATGGGG63500.029.6373714
GAGTACT68800.027.88866612-13
ATGGGAG35250.027.0108955
GTGGTAT17450.026.9703371
AGAGTAC128150.026.34715510-11
CAGAGTA133850.026.11415510-11
Produced by FastQC (version 0.11.5)