FastQCFastQC Report
Fri 27 May 2016
SRR940107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366233
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34250.055.0859031
TCAACGC39650.046.2317284
ATCAACG40050.046.0671963
AACGCAG42100.043.3898286
CAACGCA42750.042.878355
TATCAAC49300.038.268812
GTACATG90350.037.4163971
TACATGG94100.035.2288252
ACGCAGA52300.034.6948937
ACATGGG94550.034.6205333
GTACCGT2550.032.6677676
CGCAGAG56700.031.3762748
CATGGGG64750.028.310244
GTATAGG6850.026.0658841
GGTATCA41300.025.9396551
TACCGTA1150.001005798525.866617
CATGGGA40550.024.8043844
GCAGAGT68950.024.0759379
ATGGGGA38750.023.3450785
GAGTACA71100.022.9362681
Produced by FastQC (version 0.11.5)